PMID- 26795825
OWN - NLM
STAT- MEDLINE
DCOM- 20160505
LR  - 20160122
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 57
IP  - 1
DP  - 2016 Jan 1
TI  - Intervisit Repeatability of Retinal Blood Oximetry and Total Retinal Blood Flow 
      Under Varying Systemic Blood Gas Oxygen Saturations.
PG  - 188-97
LID - 10.1167/iovs.15-17908 [doi]
AB  - PURPOSE: The aim of the study is to validate and calibrate the Doppler 
      spectral-domain optical coherence tomography (SD-OCT) derived total retinal blood 
      flow (TRBF) and metabolic hyperspectral retinal camera (MHRC) derived oxygen 
      saturation (SO2) of major retinal vessels in human volunteers using a novel and 
      exact provocation technique (RespirAct) that allows the precise control of the 
      end-tidal partial pressure of oxygen (PETO2). Between visit repeatability of the 
      TRBF and retinal blood SO2 also were studied. METHODS: One eye of 11 young 
      healthy subjects was chosen randomly for the study. Total retinal blood flow and 
      retinal SO2 measurements were obtained under conditions of normoxia, hyperoxia, 
      and hypoxia. The order of hyperoxia and hypoxia was randomized between subjects. 
      The measurements were repeated after a week interval. RESULTS: When the arterial 
      PETO2 was increased from baseline (PETO2 = 100 mm Hg) to 200 and 300 mm Hg, the 
      TRBF significantly reduced (P = 0.02) from 44.60 (+/-8.9) to 40.28 (+/-8.9) and 36.23 
      (+/-4.6) muL/min. Lowering the arterial PETO2, from baseline to 80, 60, and 50 mm 
      Hg, TRBF significantly increased (P = 0.04) from 43.17 (+/-12.7) to 45.19 (+/-5.5), 
      49.71 (+/-13.4), and 52.89 (+/-10.9) muL/min with simultaneous reduction in the 
      arterial blood SO2 content from 99.3% (+/-5.8) to 95.6% (+/-5.1), 89.6% (+/-2.8), and 
      83.3% (+/-3.9), respectively (P = 0.00). The coefficient of repeatability (COR) of 
      TRBF, retinal arterial, and venous blood SO2 values are 21.8 muL/min, 18.4%, and 
      15.2%, respectively. CONCLUSIONS: Total retinal blood flow and retinal blood SO2 
      measurements performed under safe levels of hypoxia and hyperoxia were repeatable 
      in healthy adults over the range of SO2 investigated.
FAU - Rose, Kalpana
AU  - Rose K
AD  - School of Optometry and Vision Science, University of Waterloo, Waterloo, 
      Ontario, Canada.
FAU - Kulasekara, Susith I
AU  - Kulasekara SI
AD  - Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, 
      Ontario, Canada.
FAU - Hudson, Christopher
AU  - Hudson C
AD  - School of Optometry and Vision Science, University of Waterloo, Waterloo, 
      Ontario, Canada 2Department of Ophthalmology and Vision Sciences, University of 
      Toronto, Toronto, Ontario, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adult
MH  - Female
MH  - Humans
MH  - Laser-Doppler Flowmetry/*methods
MH  - Male
MH  - Oximetry/*methods
MH  - Oxygen/*blood
MH  - *Oxygen Consumption
MH  - Reference Values
MH  - Regional Blood Flow/*physiology
MH  - Retinal Vessels/cytology/*physiology
MH  - Tomography, Optical Coherence/methods
EDAT- 2016/01/23 06:00
MHDA- 2016/05/06 06:00
CRDT- 2016/01/23 06:00
PHST- 2016/01/23 06:00 [entrez]
PHST- 2016/01/23 06:00 [pubmed]
PHST- 2016/05/06 06:00 [medline]
AID - 2484502 [pii]
AID - 10.1167/iovs.15-17908 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2016 Jan 1;57(1):188-97. doi: 10.1167/iovs.15-17908.