PMID- 26797582
OWN - NLM
STAT- MEDLINE
DCOM- 20170113
LR  - 20170113
IS  - 1532-3129 (Electronic)
IS  - 0021-9975 (Linking)
VI  - 154
IP  - 2-3
DP  - 2016 Feb-Apr
TI  - Differential Expression of Porcine Reproductive and Respiratory Syndrome Virus 
      (PRRSV) Open Reading Frame 5, but not Apoptogenic Cytokines, Contribute to Severe 
      Respiratory Disease in Pigs Infected with Type 2 PRRSV Compared with Pigs 
      Infected with Type 1 PRRSV.
PG  - 243-52
LID - S0021-9975(15)00341-2 [pii]
LID - 10.1016/j.jcpa.2015.12.002 [doi]
AB  - The aim of this study was to compare the expression of open reading frame 5 
      (ORF5) of porcine reproductive and respiratory syndrome virus (PRRSV) and 
      apoptogenic cytokines in the lungs from pigs infected with type 1 and type 2 
      PRRSV. Microscopical lung lesion scores and the mean number of apoptotic cells 
      were significantly (P <0.05) higher in pigs with type 2 PRRSV infection than in 
      those with type 1 PRRSV infection. The score for the mean number of PRRSV 
      ORF5-positive cells per unit area of lung was significantly (P <0.05) higher in 
      pigs with type 2 PRRSV infection. There were no significant differences in the 
      expression of tumour necrosis factor-alpha and interleukin-1 in lung tissues between 
      type 1 and type 2 PRRSV-infected pigs. The severity of microscopical lung lesions 
      and the number of apoptotic cells correlated well with the number of PRRSV 
      ORF5-positive cells. Therefore, differential expression of PRRSV ORF5, but not 
      apoptogenic cytokines, may attribute to the severity of lung lesions and 
      apoptosis in lungs in PRRSV infection. These results suggest that expression of 
      PRRSV ORF5 may be a critical determinant for different virulence between PRRSV 
      genotypes in terms of respiratory disease.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Han, K
AU  - Han K
AD  - College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, 
      Gwanak-gu, Seoul, Republic of Korea.
FAU - Lee, J
AU  - Lee J
AD  - College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, 
      Gwanak-gu, Seoul, Republic of Korea; General Animal Health Division, Ministry of 
      Agriculture, Food and Rural Affairs, 94 Dasom 2-ro, Government Complex-Sejong, 
      Sejong-si, Republic of Korea.
FAU - Park, C
AU  - Park C
AD  - College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, 
      Gwanak-gu, Seoul, Republic of Korea.
FAU - Choi, K
AU  - Choi K
AD  - College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, 
      Gwanak-gu, Seoul, Republic of Korea.
FAU - Jeong, J
AU  - Jeong J
AD  - College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, 
      Gwanak-gu, Seoul, Republic of Korea.
FAU - Park, S-J
AU  - Park SJ
AD  - College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, 
      Gwanak-gu, Seoul, Republic of Korea.
FAU - Kang, I
AU  - Kang I
AD  - College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, 
      Gwanak-gu, Seoul, Republic of Korea.
FAU - Chae, C
AU  - Chae C
AD  - College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, 
      Gwanak-gu, Seoul, Republic of Korea. Electronic address: swine@snu.ac.kr.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160112
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Cytokines/metabolism
MH  - Genotype
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/metabolism/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*pathogenicity
MH  - Real-Time Polymerase Chain Reaction
MH  - Swine
MH  - Virulence
OTO - NOTNLM
OT  - apoptosis
OT  - cytokine
OT  - open reading frame 5
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2016/01/23 06:00
MHDA- 2017/01/14 06:00
CRDT- 2016/01/23 06:00
PHST- 2015/09/08 00:00 [received]
PHST- 2015/11/30 00:00 [revised]
PHST- 2015/12/04 00:00 [accepted]
PHST- 2016/01/23 06:00 [entrez]
PHST- 2016/01/23 06:00 [pubmed]
PHST- 2017/01/14 06:00 [medline]
AID - S0021-9975(15)00341-2 [pii]
AID - 10.1016/j.jcpa.2015.12.002 [doi]
PST - ppublish
SO  - J Comp Pathol. 2016 Feb-Apr;154(2-3):243-52. doi: 10.1016/j.jcpa.2015.12.002. 
      Epub 2016 Jan 12.