PMID- 26800180
OWN - NLM
STAT- MEDLINE
DCOM- 20160719
LR  - 20181202
IS  - 1942-4434 (Electronic)
IS  - 0882-2786 (Linking)
VI  - 31
IP  - 1
DP  - 2016 Jan-Feb
TI  - Multiple Inoculations of Bone Marrow Stromal Cells into Beta-Tricalcium 
      Phosphate/Chitosan Scaffolds Enhances the Formation and Reconstruction of New 
      Bone.
PG  - 204-15
LID - 10.11607/jomi.4024 [doi]
AB  - PURPOSE: To compare the effects of different cell inoculation strategies on 
      cellular attachment and viability as well as on osteogenic differentiation 
      capacity and subsequent bone graft functionality after implantation of 
      cellularized scaffolds in a mandibular defect rabbit model. MATERIALS AND 
      METHODS: Bone marrow stromal cells (BMSCs) were inoculated into the scaffolds of 
      beta-tricalcium phosphate/chitosan (beta-TCP/CS) either once or multiple times and 
      their efficiency assessed based on cell viability, initial seeding efficiency, 
      alkaline phosphatase activity in cell lysates, mineralization activity in 
      cryosections of cell-seeded scaffolds by von Kossa staining, and osteogenic 
      differentiation on reverse transcriptase polymerase chain reaction. RESULTS: 
      Compared with one-time inoculation of BMSCs, multiple inoculations led to higher 
      cell viability, increased proliferation, more homogeneous distribution in the 
      scaffold, better in vitro osteogenic differentiation, and improved in vivo bone 
      repair in a rabbit model of mandibular defect. CONCLUSION: Multiple inoculations 
      have many advantages over the one-time inoculation method and should be further 
      researched for bone tissue engineering.
FAU - Cheng, Gu
AU  - Cheng G
FAU - Li, Zhi
AU  - Li Z
FAU - Xing, Xin
AU  - Xing X
FAU - Li, Dian-Qi
AU  - Li DQ
FAU - Li, Zu-Bing
AU  - Li ZB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Oral Maxillofac Implants
JT  - The International journal of oral & maxillofacial implants
JID - 8611905
RN  - 0 (Calcium Phosphates)
RN  - 0 (Osteonectin)
RN  - 0 (Osteoprotegerin)
RN  - 0 (beta-tricalcium phosphate)
RN  - 104982-03-8 (Osteocalcin)
RN  - 9007-49-2 (DNA)
RN  - 9012-76-4 (Chitosan)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/analysis
MH  - Animals
MH  - Bone Regeneration/*physiology
MH  - Calcification, Physiologic/physiology
MH  - Calcium Phosphates/*chemistry
MH  - Cell Adhesion/physiology
MH  - Cell Count
MH  - Cell Culture Techniques
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation
MH  - Cell Survival/physiology
MH  - Chitosan/*chemistry
MH  - DNA/analysis
MH  - Disease Models, Animal
MH  - Male
MH  - Mandibular Diseases/surgery
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/*physiology
MH  - Microscopy, Confocal
MH  - Osteocalcin/analysis
MH  - Osteogenesis/*physiology
MH  - Osteonectin/analysis
MH  - Osteoprotegerin/analysis
MH  - Rabbits
MH  - Tissue Scaffolds/*chemistry
EDAT- 2016/01/23 06:00
MHDA- 2016/07/20 06:00
CRDT- 2016/01/23 06:00
PHST- 2016/01/23 06:00 [entrez]
PHST- 2016/01/23 06:00 [pubmed]
PHST- 2016/07/20 06:00 [medline]
AID - 10.11607/jomi.4024 [doi]
PST - ppublish
SO  - Int J Oral Maxillofac Implants. 2016 Jan-Feb;31(1):204-15. doi: 
      10.11607/jomi.4024.