PMID- 26801213
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20161230
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 11
IP  - 2
DP  - 2016 Jun 22
TI  - Imaging of amyloid-beta in Alzheimer's disease transgenic mouse brains with ToF-SIMS 
      using immunoliposomes.
PG  - 02A312
LID - 10.1116/1.4940215 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been proven to 
      successfully image different kinds of molecules, especially a variety of lipids, 
      in biological samples. Proteins, however, are difficult to detect as specific 
      entities with this method due to extensive fragmentation. To circumvent this 
      issue, the authors present in this work a method developed for detection of 
      proteins using antibody-conjugated liposomes, so called immunoliposomes, which 
      are able to bind to the specific protein of interest. In combination with the 
      capability of ToF-SIMS to detect native lipids in tissue samples, this method 
      opens up the opportunity to analyze many different biomolecules, both lipids and 
      proteins, at the same time, with high spatial resolution. The method has been 
      applied to detect and image the distribution of amyloid-beta (Abeta), a biologically 
      relevant peptide in Alzheimer's disease (AD), in transgenic mouse brain tissue. 
      To ensure specific binding, the immunoliposome binding was verified on a model 
      surface using quartz crystal microbalance with dissipation monitoring. The 
      immunoliposome binding was also investigated on tissue sections with fluorescence 
      microscopy, and compared with conventional immunohistochemistry using primary and 
      secondary antibodies, demonstrating specific binding to Abeta. Using ToF-SIMS 
      imaging, several endogenous lipids, such as cholesterol and sulfatides, were also 
      detected in parallel with the immunoliposome-labeled Abeta deposits, which is an 
      advantage compared to fluorescence microscopy. This method can thus potentially 
      provide further information about lipid-protein interactions, which is important 
      to understand the mechanisms of neurodegeneration in AD.
FAU - Carlred, Louise
AU  - Carlred L
AD  - SP Technical Research Institute of Sweden, Boras SE-501 15, Sweden and Department 
      of Applied Physics, Chalmers University of Technology, SE-412 96 Goteborg, 
      Sweden.
FAU - Vukojevic, Vladana
AU  - Vukojevic V
AD  - Department of Clinical Neuroscience, Karolinska Institutet and Center for 
      Molecular Medicine (CMM), Karolinska University Hospital, SE-171 76 Stockholm, 
      Sweden.
FAU - Johansson, Bjorn
AU  - Johansson B
AD  - Department of Molecular Medicine and Surgery, Karolinska Institutet and Center 
      for Molecular Medicine (CMM), Karolinska University Hospital, SE-171 76 
      Stockholm, Sweden.
FAU - Schalling, Martin
AU  - Schalling M
AD  - Department of Molecular Medicine and Surgery, Karolinska Institutet and Center 
      for Molecular Medicine (CMM), Karolinska University Hospital, SE-171 76 
      Stockholm, Sweden.
FAU - Hook, Fredrik
AU  - Hook F
AD  - Department of Applied Physics, Chalmers University of Technology, SE-412 96 
      Goteborg, Sweden.
FAU - Sjovall, Peter
AU  - Sjovall P
AD  - SP Technical Research Institute of Sweden, Boras SE-501 15, Sweden and Department 
      of Applied Physics, Chalmers University of Technology, SE-412 96 Goteborg, 
      Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160622
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Liposomes)
SB  - IM
MH  - Alzheimer Disease/*pathology
MH  - Amyloid beta-Peptides/*analysis
MH  - Animals
MH  - Brain/*pathology
MH  - Disease Models, Animal
MH  - *Immunohistochemistry
MH  - *Liposomes
MH  - Mice, Transgenic
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2016/01/24 06:00
MHDA- 2016/10/14 06:00
CRDT- 2016/01/24 06:00
PHST- 2016/01/24 06:00 [entrez]
PHST- 2016/01/24 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
AID - 10.1116/1.4940215 [doi]
PST - epublish
SO  - Biointerphases. 2016 Jun 22;11(2):02A312. doi: 10.1116/1.4940215.