PMID- 26801580
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20191210
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 82
IP  - 7
DP  - 2016 Jan 22
TI  - Evaluation of the Efficacy of Methyl Bromide in the Decontamination of Building 
      and Interior Materials Contaminated with Bacillus anthracis Spores.
PG  - 2003-2011
LID - 10.1128/AEM.03445-15 [doi]
AB  - The primary goal of this study was to determine the conditions required for the 
      effective inactivation of Bacillus anthracis spores on materials by using methyl 
      bromide (MeBr) gas. Another objective was to obtain comparative decontamination 
      efficacy data with three avirulent microorganisms to assess their potential for 
      use as surrogates for B. anthracis Ames. Decontamination tests were conducted 
      with spores of B. anthracis Ames and Geobacillus stearothermophilus, B. anthracis 
      NNR1Delta1, and B. anthracis Sterne inoculated onto six different materials. 
      Experimental variables included temperature, relative humidity (RH), MeBr 
      concentration, and contact time. MeBr was found to be an effective decontaminant 
      under a number of conditions. This study highlights the important role that RH 
      has when fumigation is performed with MeBr. There were no tests in which a 
      >/=6-log10 reduction (LR) of B. anthracis Ames was achieved on all materials when 
      fumigation was done at 45% RH. At 75% RH, an increase in the temperature, the 
      MeBr concentration, or contact time generally improved the efficacy of fumigation 
      with MeBr. This study provides new information for the effective use of MeBr at 
      temperatures and RH levels lower than those that have been recommended 
      previously. The study also provides data to assist with the selection of an 
      avirulent surrogate for B. anthracis Ames spores when additional tests with MeBr 
      are conducted.
CI  - Copyright (c) 2016 Wood et al.
FAU - Wood, Joseph P
AU  - Wood JP
AUID- ORCID: 0000-0001-6316-9418
AD  - U.S. Environmental Protection Agency, Office of Research and Development, 
      National Homeland Security Research Center, Research Triangle Park, North 
      Carolina, USA wood.joe@epa.gov.
FAU - Wendling, Morgan
AU  - Wendling M
AD  - Battelle Memorial Institute, West Jefferson, Ohio, USA.
FAU - Richter, William
AU  - Richter W
AD  - Battelle Memorial Institute, West Jefferson, Ohio, USA.
FAU - Lastivka, Andrew
AU  - Lastivka A
AD  - Battelle Memorial Institute, West Jefferson, Ohio, USA.
FAU - Mickelsen, Leroy
AU  - Mickelsen L
AD  - U.S. Environmental Protection Agency, Office of Emergency Management, Research 
      Triangle Park, North Carolina, USA.
LA  - eng
GR  - HIR 10-001/HX/HSRD VA/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160122
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Disinfectants)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 9V42E1Z7B6 (methyl bromide)
SB  - IM
MH  - Bacillus anthracis/*drug effects/growth & development
MH  - Construction Materials/*microbiology
MH  - Decontamination/instrumentation/*methods
MH  - Disinfectants/chemistry/*pharmacology
MH  - Fumigation
MH  - Hydrocarbons, Brominated/chemistry/*pharmacology
MH  - Spores, Bacterial/*drug effects/growth & development
PMC - PMC4807506
EDAT- 2016/01/24 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/03/21
CRDT- 2016/01/24 06:00
PHST- 2015/10/28 00:00 [received]
PHST- 2016/01/08 00:00 [accepted]
PHST- 2016/01/24 06:00 [entrez]
PHST- 2016/01/24 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/03/21 00:00 [pmc-release]
AID - AEM.03445-15 [pii]
AID - 03445-15 [pii]
AID - 10.1128/AEM.03445-15 [doi]
PST - epublish
SO  - Appl Environ Microbiol. 2016 Jan 22;82(7):2003-2011. doi: 10.1128/AEM.03445-15.