PMID- 26802177
OWN - NLM
STAT- MEDLINE
DCOM- 20160809
LR  - 20220408
IS  - 1554-6578 (Electronic)
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 75
IP  - 2
DP  - 2016 Feb
TI  - A Compact Blast-Induced Traumatic Brain Injury Model in Mice.
PG  - 183-96
AB  - Blast-induced traumatic brain injury (bTBI) is a common injury on the battlefield 
      and often results in permanent cognitive and neurological abnormalities. We 
      report a novel compact device that creates graded bTBI in mice. The injury 
      severity can be controlled by precise pressures that mimic Friedlander shockwave 
      curves. The mouse head was stabilized with a head fixator, and the body was 
      protected with a metal shield; shockwave durations were 3 to 4 milliseconds. 
      Reflective shockwave peak readings at the position of the mouse head were 12 6 
      2.6 psi, 50 6 20.3 psi, and 100 6 33.1 psi at 100, 200, and 250 psi predetermined 
      driver chamber pressures, respectively. The bTBIs of 250 psi caused 80% 
      mortality, which decreased to 27% with the metal shield. Brain and lung damage 
      depended on the shockwave duration and amplitude. Cognitive deficits were 
      assessed using the Morris water maze, Y-maze, and open-field tests. Pathological 
      changes in the brain included disruption of the blood-brain barrier, multifocal 
      neuronal and axonal degeneration, and reactive gliosis assessed by Evans Blue dye 
      extravasation, silver and Fluoro-Jade B staining, and glial fibrillary acidic 
      protein immunohistochemistry, respectively. Behavioral and pathological changes 
      were injury severity-dependent. This mouse bTBI model may be useful for 
      investigating injury mechanisms and therapeutic strategies associated with bTBI.
FAU - Wang, Hongxing
AU  - Wang H
FAU - Zhang, Yi Ping
AU  - Zhang YP
FAU - Cai, Jun
AU  - Cai J
FAU - Shields, Lisa B E
AU  - Shields LB
FAU - Tuchek, Chad A
AU  - Tuchek CA
FAU - Shi, Riyi
AU  - Shi R
FAU - Li, Jianan
AU  - Li J
FAU - Shields, Christopher B
AU  - Shields CB
FAU - Xu, Xiao-Ming
AU  - Xu XM
LA  - eng
GR  - 1R01 NS059622/NS/NINDS NIH HHS/United States
GR  - 1R01 NS073636/NS/NINDS NIH HHS/United States
GR  - R01 NS050243/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Acute Lung Injury/pathology
MH  - Animals
MH  - Axons/pathology
MH  - Behavior, Animal
MH  - Blast Injuries/mortality/*pathology/psychology
MH  - Blood-Brain Barrier/pathology
MH  - Brain Edema/pathology
MH  - Brain Injuries/mortality/*pathology/psychology
MH  - Disease Models, Animal
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Gliosis/pathology
MH  - Male
MH  - Maze Learning
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Motor Activity
MH  - Neurons/pathology
MH  - Pressure
PMC - PMC6366653
EDAT- 2016/01/24 06:00
MHDA- 2016/08/10 06:00
PMCR- 2017/02/01
CRDT- 2016/01/24 06:00
PHST- 2016/01/24 06:00 [entrez]
PHST- 2016/01/24 06:00 [pubmed]
PHST- 2016/08/10 06:00 [medline]
PHST- 2017/02/01 00:00 [pmc-release]
AID - nlv019 [pii]
AID - 10.1093/jnen/nlv019 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 2016 Feb;75(2):183-96. doi: 10.1093/jnen/nlv019.