PMID- 26804033
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20231213
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 32
DP  - 2016 Apr
TI  - Organ- and species-specific biological activity of rosmarinic acid.
PG  - 261-8
LID - S0887-2333(16)30009-1 [pii]
LID - 10.1016/j.tiv.2016.01.009 [doi]
AB  - Rosmarinic acid (RA), a compound found in several plant species, has beneficial 
      properties, including anti-inflammatory and antibacterial effects. We 
      investigated the toxicity, anti-inflammatory, and antifibrotic effects of RA 
      using precision-cut liver slices (PCLS) and precision-cut intestinal slices 
      (PCIS) prepared from human, mouse, and rat tissue. PCLS and PCIS were cultured up 
      to 48 h in the absence or presence of RA. Gene expression of the inflammatory 
      markers: IL-6, IL-8/CXCL1/KC, and IL-1beta, as well as the fibrosis markers: 
      pro-collagen 1a1, heat shock protein 47, alpha-smooth muscle actin, fibronectin (Fn2) 
      and plasminogen activator inhibitor-1 (PAI-1) were evaluated by qPCR. RA was only 
      toxic in murine PCIS. RA failed to mitigate the inflammatory response in most 
      models, while it clearly reduced IL-6 and CXCL1/KC gene expression in murine PCIS 
      at non-toxic concentrations. With regard to fibrosis, RA decreased the gene 
      levels of Fn2 and PAI-1 in murine PCLS, and Fn2 in murine PCIS. Yet, no effect 
      was observed on the gene expression of fibrosis markers in human and rat PCIS. In 
      conclusion, we observed clear organ- and species-specific effects of RA. RA had 
      little influence on inflammation. However, our study further establishes RA as a 
      potential candidate for the treatment of liver fibrosis.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Iswandana, R
AU  - Iswandana R
AD  - Pharmaceutical Technology and Biopharmacy, Department of Pharmacy, University of 
      Groningen, The Netherlands; Faculty of Pharmacy, Universitas Indonesia, 
      Indonesia. Electronic address: raditya.iswandana@rug.nl.
FAU - Pham, B T
AU  - Pham BT
AD  - Pharmaceutical Technology and Biopharmacy, Department of Pharmacy, University of 
      Groningen, The Netherlands; Department of Pharmaceutics, Hanoi University of 
      Pharmacy, Vietnam. Electronic address: b.t.pham@rug.nl.
FAU - van Haaften, W T
AU  - van Haaften WT
AD  - Pharmaceutical Technology and Biopharmacy, Department of Pharmacy, University of 
      Groningen, The Netherlands. Electronic address: w.t.van.haaften@umcg.nl.
FAU - Luangmonkong, T
AU  - Luangmonkong T
AD  - Pharmaceutical Technology and Biopharmacy, Department of Pharmacy, University of 
      Groningen, The Netherlands; Department of Pharmacology, Faculty of Pharmacy, 
      Mahidol University, Thailand. Electronic address: t.luangmonkong@rug.nl.
FAU - Oosterhuis, D
AU  - Oosterhuis D
AD  - Pharmaceutical Technology and Biopharmacy, Department of Pharmacy, University of 
      Groningen, The Netherlands. Electronic address: d.oosterhuis@rug.nl.
FAU - Mutsaers, H A M
AU  - Mutsaers HA
AD  - Pharmaceutical Technology and Biopharmacy, Department of Pharmacy, University of 
      Groningen, The Netherlands. Electronic address: h.a.m.mutsaers@rug.nl.
FAU - Olinga, P
AU  - Olinga P
AD  - Pharmaceutical Technology and Biopharmacy, Department of Pharmacy, University of 
      Groningen, The Netherlands. Electronic address: p.olinga@rug.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160119
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Cinnamates)
RN  - 0 (Cytokines)
RN  - 0 (Depsides)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology/toxicity
MH  - Cinnamates/*pharmacology/toxicity
MH  - Cytokines/genetics
MH  - Depsides/*pharmacology/toxicity
MH  - Gene Expression/drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Jejunum/*drug effects/metabolism
MH  - Liver/*drug effects/metabolism/pathology
MH  - Liver Cirrhosis/genetics
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Rats, Wistar
MH  - Species Specificity
MH  - Rosmarinic Acid
OTO - NOTNLM
OT  - Anti-inflammatory
OT  - Antifibrotic
OT  - Precision-cut intestinal slices
OT  - Precision-cut liver slices
OT  - Rosmarinic acid
OT  - Toxicity
EDAT- 2016/01/26 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/01/26 06:00
PHST- 2015/11/06 00:00 [received]
PHST- 2016/01/11 00:00 [revised]
PHST- 2016/01/18 00:00 [accepted]
PHST- 2016/01/26 06:00 [entrez]
PHST- 2016/01/26 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0887-2333(16)30009-1 [pii]
AID - 10.1016/j.tiv.2016.01.009 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2016 Apr;32:261-8. doi: 10.1016/j.tiv.2016.01.009. Epub 2016 
      Jan 19.