PMID- 26806731
OWN - NLM
STAT- MEDLINE
DCOM- 20160426
LR  - 20181202
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 36
IP  - 1
DP  - 2016 Jan
TI  - [Role of nuclear factor of activated T cells-2 in high mobility protein box-1 
      release in human monocytic THP-1 cells in vitro].
PG  - 8-12
AB  - OBJECTIVE: To investigate the role of nuclear factor of activated T cells -2 
      (NFAT2) in release of high mobility protein box-1 (HMGB1) from human monocytic 
      THP-1 cells in vitro. METHODS: The level of HMGB1 release from THP-1 cells in 
      response to lipopolysaccharide (LPS) stimulation was examined by Western blotting 
      and enzyme-linked immunosorbent assay (ELISA). The effect of LPS stimulation on 
      NFAT2 and HMGB1 interaction in the cytoplasm was observed by immunoprecipitation 
      assay. HMGB1 production and release was detected in cells with specific small 
      interfering RNA (siRNA)-mediated suppression of NFAT2 expression. RESULTS: LPS 
      stimulated HMGB1 release from THP-1 cells. As LPS stimulation prolonged, HMGB1 
      concentration increased in the cell culture supernatant and decreased in the 
      cytoplasm, and the binding between NFAT2 and HMGB1 was not detected in the cell 
      nuclei. NFAT2 suppression by the siRNA plasmid resulted in increased HMGB1 level 
      in the cell culture supernatant. CONCLUSION: NFAT2 can inhibit HMGB1 release from 
      THP-1 cells in vitro.
FAU - Zhao, Qing
AU  - Zhao Q
AD  - Dapartment of Gastroenterology in Nanlou, the Chinese PLA General Hospital, 
      Beijing 100853, China.E-mail: zq0405@sina.com.
FAU - Wang, Li
AU  - Wang L
FAU - Hu, Jie
AU  - Hu J
FAU - Liu, Hui
AU  - Liu H
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (NFATC1 protein, human)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Nucleus
MH  - Cytoplasm/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HMGB1 Protein/*metabolism
MH  - Humans
MH  - Lipopolysaccharides
MH  - Monocytes/*metabolism
MH  - NFATC Transcription Factors/*metabolism
EDAT- 2016/01/26 06:00
MHDA- 2016/04/27 06:00
CRDT- 2016/01/26 06:00
PHST- 2016/01/26 06:00 [entrez]
PHST- 2016/01/26 06:00 [pubmed]
PHST- 2016/04/27 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2016 Jan;36(1):8-12.