PMID- 26807018
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160125
LR  - 20201001
IS  - 1226-4512 (Print)
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Linking)
VI  - 20
IP  - 1
DP  - 2016 Jan
TI  - cAMP induction by ouabain promotes endothelin-1 secretion via MAPK/ERK signaling 
      in beating rabbit atria.
PG  - 9-14
LID - 10.4196/kjpp.2016.20.1.9 [doi]
AB  - Adenosine 3',5'-cyclic monophosphate (cAMP) participates in the regulation of 
      numerous cellular functions, including the Na(+)-K(+)-ATPase (sodium pump). 
      Ouabain, used in the treatment of several heart diseases, is known to increase 
      cAMP levels but its effects on the atrium are not understood. The aim of the 
      present study was to examine the effect of ouabain on the regulation of atrial 
      cAMP production and its roles in atrial endothelin-1 (ET-1) secretion in isolated 
      perfused beating rabbit atria. Our results showed that ouabain (3.0 micromol/L) 
      significantly increased atrial dynamics and cAMP levels during recovery period. 
      The ouabain-increased atrial dynamics was blocked by KB-R7943 (3.0 micromol/L), an 
      inhibitor for reverse mode of Na(+)-Ca(2+) exchangers (NCX), but did not by 
      L-type Ca(2+) channel blocker nifedipine (1.0 micromol/L) or protein kinase A (PKA) 
      selective inhibitor H-89 (3.0 micromol/L). Ouabain also enhanced atrial intracellular 
      cAMP production in response to forskolin and theophyline (100.0 micromol/L), an 
      inhibitor of phosphodiesterase, potentiated the ouabain-induced increase in cAMP. 
      Ouabain and 8-Bromo-cAMP (0.5 micromol/L) markedly increased atrial ET-1 secretion, 
      which was blocked by H-89 and by PD98059 (30 micromol/L), an inhibitor of 
      extracellular-signal-regulated kinase (ERK) without changing ouabain-induced 
      atrial dynamics. Our results demonstrated that ouabain increases atrial cAMP 
      levels and promotes atrial ET-1 secretion via the mitogen-activated protein 
      kinase (MAPK)/ERK signaling pathway. These findings may explain the development 
      of cardiac hypertrophy in response to digitalis-like compounds.
FAU - Peng, Li-Qun
AU  - Peng LQ
AD  - Department of Physiology, School of Medicine, Yanbian University, Yanji 133-002, 
      China.; KeErQinQu First People's Hospital, Tongliao, Neimenggu 028050, China.
FAU - Li, Ping
AU  - Li P
AD  - Department of Physiology, School of Medicine, Yanbian University, Yanji 133-002, 
      China.
FAU - Zhang, Qiu-Li
AU  - Zhang QL
AD  - Department of Physiology, School of Medicine, Yanbian University, Yanji 133-002, 
      China.
FAU - Hong, Lan
AU  - Hong L
AD  - Department of Physiology, School of Medicine, Yanbian University, Yanji 133-002, 
      China.
FAU - Liu, Li-Ping
AU  - Liu LP
AD  - Department of Physiology, School of Medicine, Yanbian University, Yanji 133-002, 
      China.
FAU - Cui, Xun
AU  - Cui X
AD  - Department of Physiology, School of Medicine, Yanbian University, Yanji 133-002, 
      China.; Key Laboratory of Organism Functional Factors of the Changbai Mountain, 
      Ministry of Education, Yanbian University, Yanji 133-002, China.; Cellular 
      Function Research Center, Yanbian University, Yanji 133-002, China.
FAU - Cui, Bai-Ri
AU  - Cui BR
AD  - Department of Physiology, School of Medicine, Yanbian University, Yanji 133-002, 
      China.; Institue of Clinical Medicine, Yanbian University, Yanji 133000, China.
LA  - eng
PT  - Journal Article
DEP - 20151231
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
PMC - PMC4722196
OTO - NOTNLM
OT  - Adenosine 3',5'-cyclic monophosphate (cAMP)
OT  - Endothelin-1 (ET-1)
OT  - Mitogen-activated protein kinase (MAPK)
OT  - Ouabain
EDAT- 2016/01/26 06:00
MHDA- 2016/01/26 06:01
PMCR- 2016/01/01
CRDT- 2016/01/26 06:00
PHST- 2014/12/11 00:00 [received]
PHST- 2015/03/31 00:00 [revised]
PHST- 2015/04/16 00:00 [accepted]
PHST- 2016/01/26 06:00 [entrez]
PHST- 2016/01/26 06:00 [pubmed]
PHST- 2016/01/26 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.4196/kjpp.2016.20.1.9 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2016 Jan;20(1):9-14. doi: 10.4196/kjpp.2016.20.1.9. 
      Epub 2015 Dec 31.