PMID- 26808725
OWN - NLM
STAT- MEDLINE
DCOM- 20170419
LR  - 20221207
IS  - 1423-002X (Electronic)
IS  - 0378-7346 (Linking)
VI  - 81
IP  - 5
DP  - 2016
TI  - c-myc Gene Copy Number Variation in Cervical Exfoliated Cells Detected on 
      Fluorescence in situ Hybridization for Cervical Cancer Screening.
PG  - 416-23
LID - 10.1159/000442286 [doi]
AB  - PURPOSE: This study assessed the clinical significance of c-myc gene copy number 
      gain detected by fluorescence in situ hybridization (FISH) in the prediction of 
      cervical intraepithelial neoplasia (CIN) progression. METHODS: We retrospectively 
      investigated 140 Thinprep cytologic test (TCT) specimens that were 
      histopathologically diagnosed with various stages of cervical neoplasia or 
      malignancy. The specimens were subjected to TCT, human papillomavirus (HPV) 
      testing, and FISH analysis with a c-myc-specific probe. The diagnostic 
      reliability of these methods in determining progression was assessed according to 
      sensitivity, specificity, and kappa coefficients. RESULTS: The gene copy number gain 
      of c-myc was significantly higher in the cervical lesion of advanced histologic 
      grade (p < 0.001). For CIN2+ lesions, the sensitivities of TCT, HPV DNA testing, 
      and FISH analysis were 72.3, 92.1, and 64.5%, respectively; the specificities 
      were 81.3, 57.8, and 93.8%, respectively (p < 0.001). The kappa coefficients between 
      the c-myc gene test and either the TCT or the HPV DNA test were 0.538 and 0.399, 
      respectively (p < 0.001). CONCLUSIONS: FISH analysis of the c-myc oncogene could 
      be a useful adjunct screening method for the early diagnosis of high-grade 
      cervical lesions. Moreover, c-myc may be a new molecular biomarker for the early 
      diagnosis of cervical lesion progression.
CI  - (c) 2016 S. Karger AG, Basel.
FAU - Zhao, Wei-Hong
AU  - Zhao WH
AD  - Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical 
      University, Taiyuan, China.
FAU - Hao, Min
AU  - Hao M
FAU - Cheng, Xiao-Tong
AU  - Cheng XT
FAU - Yang, Xin
AU  - Yang X
FAU - Wang, Zhi-Lian
AU  - Wang ZL
FAU - Cheng, Ke-Yan
AU  - Cheng KY
FAU - Liu, Fang-Li
AU  - Liu FL
FAU - Bai, Yao-Xian
AU  - Bai YX
LA  - eng
PT  - Journal Article
DEP - 20160126
PL  - Switzerland
TA  - Gynecol Obstet Invest
JT  - Gynecologic and obstetric investigation
JID - 7900587
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/genetics
MH  - Carcinoma, Squamous Cell/genetics/*pathology
MH  - Cytological Techniques
MH  - *DNA Copy Number Variations
MH  - *Early Detection of Cancer
MH  - Female
MH  - Genes, myc/*genetics
MH  - Genetic Markers/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Papillomaviridae/isolation & purification
MH  - Papillomavirus Infections/diagnosis
MH  - Reproducibility of Results
MH  - Retrospective Studies
MH  - Uterine Cervical Neoplasms/genetics/*pathology
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/genetics/pathology
EDAT- 2016/01/26 06:00
MHDA- 2017/04/20 06:00
CRDT- 2016/01/26 06:00
PHST- 2015/07/14 00:00 [received]
PHST- 2015/11/05 00:00 [accepted]
PHST- 2016/01/26 06:00 [entrez]
PHST- 2016/01/26 06:00 [pubmed]
PHST- 2017/04/20 06:00 [medline]
AID - 000442286 [pii]
AID - 10.1159/000442286 [doi]
PST - ppublish
SO  - Gynecol Obstet Invest. 2016;81(5):416-23. doi: 10.1159/000442286. Epub 2016 Jan 
      26.