PMID- 26809954
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 1868-601X (Electronic)
IS  - 1868-4483 (Print)
IS  - 1868-4483 (Linking)
VI  - 7
IP  - 2
DP  - 2016 Apr
TI  - Effects of Acute Stroke Serum on Non-Ischemic Cerebral and Mesenteric Vascular 
      Function.
PG  - 156-65
LID - 10.1007/s12975-016-0449-7 [doi]
AB  - We investigated the effects of circulating factors in serum obtained from 
      patients in the acute phase of different subtypes of ischemic stroke on 
      non-ischemic cerebral and mesenteric arteries, as a potential mechanism involved 
      in influencing regional perfusion and thus clinical evolution. Posterior cerebral 
      arteries (PCAs) and mesentery arteries (MAs) isolated from Wistar Kyoto rats were 
      perfused with serum from acute stroke patients with large vessel disease without 
      (LVD) or with hypertension (LVD + HTN), cardioembolism with hypertension 
      (CE + HTN), or physiologic saline as controls. Myogenic activity and nitric 
      oxide-dependent vasorelaxation were assessed after 2 h of intraluminal exposure 
      to serum. Vascular function was differentially affected by sera. Exposure to LVD 
      serum increased myogenic tone and produced endothelial dysfunction in both PCAs 
      and MAs. However, CE + HTN serum increased tone and decreased smooth muscle 
      sensitivity to NO in vessels from both vascular beds. LVD + HTN serum was 
      associated with reduced smooth muscle sensitivity to NO in vessels from both 
      vascular beds but increased tone only in PCAs. Inflammation and oxidative stress, 
      determined by measurement of high sensitivity C-reactive protein, uric acid, and 
      free 8-isoprostane, were enhanced in all the serum groups. These results 
      demonstrate vasoactive properties of acute stroke serum related to stroke 
      subtypes that could potentially contribute to the pathogenesis of early 
      hemodynamic-based clinical events.
FAU - Canavero, Isabella
AU  - Canavero I
AD  - Department of Neurological Sciences, University of Vermont College of Medicine, 
      149 Beaumont Ave., HSRF 416A, Burlington, VT, 05405, USA.
FAU - Sherburne, Helene A
AU  - Sherburne HA
AD  - Department of Neurological Sciences, University of Vermont College of Medicine, 
      149 Beaumont Ave., HSRF 416A, Burlington, VT, 05405, USA.
FAU - Tremble, Sarah M
AU  - Tremble SM
AD  - Department of Neurological Sciences, University of Vermont College of Medicine, 
      149 Beaumont Ave., HSRF 416A, Burlington, VT, 05405, USA.
FAU - Clark, Wayne M
AU  - Clark WM
AD  - Department of Neurology, Oregon Stroke Center, Oregon Health and Science 
      University, Portland, OR, USA.
FAU - Cipolla, Marilyn J
AU  - Cipolla MJ
AD  - Department of Neurological Sciences, University of Vermont College of Medicine, 
      149 Beaumont Ave., HSRF 416A, Burlington, VT, 05405, USA. 
      Marilyn.Cipolla@uvm.edu.
AD  - Department of Obstetrics, Gynecology, and Reproductive Sciences, University of 
      Vermont College of Medicine, Burlington, VT, USA. Marilyn.Cipolla@uvm.edu.
AD  - Department of Pharmacology, University of Vermont College of Medicine, 
      Burlington, VT, USA. Marilyn.Cipolla@uvm.edu.
LA  - eng
GR  - R01 NS093289/NS/NINDS NIH HHS/United States
GR  - P01 HL095488/HL/NHLBI NIH HHS/United States
GR  - R01 NS045940/NS/NINDS NIH HHS/United States
GR  - R01 NS-093289/NS/NINDS NIH HHS/United States
GR  - R01 NS-045940/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160126
PL  - United States
TA  - Transl Stroke Res
JT  - Translational stroke research
JID - 101517297
RN  - 0 (Vasodilator Agents)
RN  - 169D1260KM (Nitroprusside)
RN  - 268B43MJ25 (Uric Acid)
RN  - 27415-26-5 (8-epi-prostaglandin F2alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - B7IN85G1HY (Dinoprost)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Aged
MH  - Animals
MH  - C-Reactive Protein/metabolism
MH  - Cerebral Arteries/*pathology/physiopathology
MH  - Dinoprost/analogs & derivatives/blood
MH  - Disease Models, Animal
MH  - Female
MH  - Humans
MH  - Hypertension/complications
MH  - Male
MH  - Middle Aged
MH  - Muscle, Smooth, Vascular/drug effects/*physiopathology
MH  - Nitric Oxide/pharmacology
MH  - Nitroprusside/pharmacology
MH  - Rats
MH  - Rats, Inbred WKY
MH  - Serum/*metabolism
MH  - Splanchnic Circulation/drug effects/*physiology
MH  - Stroke/*blood/complications/*pathology
MH  - Uric Acid/blood
MH  - Vasodilator Agents/pharmacology
PMC - PMC4775304
MID - NIHMS754870
OTO - NOTNLM
OT  - Acute stroke
OT  - Circulating factors
OT  - Free 8-isoprostane
OT  - Inflammation
OT  - Oxidative stress
OT  - Stroke subtypes
COIS- Conflict of Interest None to declare.
EDAT- 2016/01/27 06:00
MHDA- 2016/12/15 06:00
PMCR- 2017/04/01
CRDT- 2016/01/27 06:00
PHST- 2015/10/30 00:00 [received]
PHST- 2016/01/08 00:00 [accepted]
PHST- 2015/12/17 00:00 [revised]
PHST- 2016/01/27 06:00 [entrez]
PHST- 2016/01/27 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - 10.1007/s12975-016-0449-7 [pii]
AID - 10.1007/s12975-016-0449-7 [doi]
PST - ppublish
SO  - Transl Stroke Res. 2016 Apr;7(2):156-65. doi: 10.1007/s12975-016-0449-7. Epub 
      2016 Jan 26.