PMID- 26811239
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20190109
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 13
DP  - 2016 Jan 25
TI  - Genetic variations in regions of bovine and bovine-like enteroviral 5'UTR from 
      cattle, Indian bison and goat feces.
PG  - 13
LID - 10.1186/s12985-016-0468-8 [doi]
LID - 13
AB  - BACKGROUND: Bovine enteroviruses (BEV) are members of the genus Enterovirus in 
      the family Picornaviridae. They are predominantly isolated from cattle feces, but 
      also are detected in feces of other animals, including goats and deer. These 
      viruses are found in apparently healthy animals, as well as in animals with 
      clinical signs and several studies reported recently suggest a potential role of 
      BEV in causing disease in animals. In this study, we surveyed the presence of BEV 
      in domestic and wild animals in Thailand, and assessed their genetic variability. 
      METHODS: Viral RNA was extracted from fecal samples of cattle, domestic goats, 
      Indian bison (gaurs), and deer. The 5' untranslated region (5'UTR) was amplified 
      by nested reverse transcription-polymerase chain reaction (RT-PCR) with primers 
      specific to BEV 5'UTR. PCR products were sequenced and analyzed phylogenetically 
      using the neighbor-joining algorithm to observe genetic variations in regions of 
      the bovine and bovine-like enteroviral 5'UTR found in this study. RESULTS: BEV 
      and BEV-like sequences were detected in the fecal samples of cattle (40/60, 67 
      %), gaurs (3/30, 10 %), and goats (11/46, 24 %). Phylogenetic analyses of the 
      partial 5'UTR sequences indicated that different BEV variants (both EV-E and EV-F 
      species) co-circulated in the domestic cattle, whereas the sequences from gaurs 
      and goats clustered according to the animal species, suggesting that these 
      viruses are host species-specific. CONCLUSIONS: Varieties of BEV and BEV-like 
      5'UTR sequences were detected in fecal samples from both domestic and wild 
      animals. To our knowledge, this is the first report of the genetic variability of 
      BEV in Thailand.
FAU - Kosoltanapiwat, Nathamon
AU  - Kosoltanapiwat N
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand. nathamon.kos@mahidol.ac.th.
FAU - Yindee, Marnoch
AU  - Yindee M
AD  - Faculty of Veterinary Science, Mahidol University, Bangkok, Thailand. 
      drfungy2000@yahoo.com.
FAU - Chavez, Irwin Fernandez
AU  - Chavez IF
AD  - Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, 
      Bangkok, Thailand. irwin.cha@mahidol.ac.th.
FAU - Leaungwutiwong, Pornsawan
AU  - Leaungwutiwong P
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand. pornsawan.lea@mahidol.ac.th.
FAU - Adisakwattana, Poom
AU  - Adisakwattana P
AD  - Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, 
      Bangkok, Thailand. poom.adi@mahidol.ac.th.
FAU - Singhasivanon, Pratap
AU  - Singhasivanon P
AD  - Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, 
      Bangkok, Thailand. pratap.sin@mahidol.ac.th.
FAU - Thawornkuno, Charin
AU  - Thawornkuno C
AD  - Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical 
      Medicine, Mahidol University, Bangkok, Thailand. charin.tha@mahidol.ac.th.
FAU - Thippornchai, Narin
AU  - Thippornchai N
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand. narin.thi@mahidol.ac.th.
FAU - Rungruengkitkun, Amporn
AU  - Rungruengkitkun A
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand. amporn.run@mahidol.ac.th.
FAU - Soontorn, Juthamas
AU  - Soontorn J
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand. juthamas_401@gmail.com.
FAU - Pearsiriwuttipong, Sasipan
AU  - Pearsiriwuttipong S
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand. am_peace_dh@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160125
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Animals
MH  - Bison
MH  - Cattle
MH  - Enterovirus, Bovine/*classification/*genetics/isolation & purification
MH  - Feces/virology
MH  - *Genetic Variation
MH  - Geography
MH  - Goats
MH  - Phylogeny
MH  - RNA, Viral
MH  - Sequence Analysis, DNA
PMC - PMC4727389
EDAT- 2016/01/27 06:00
MHDA- 2016/10/14 06:00
PMCR- 2016/01/25
CRDT- 2016/01/27 06:00
PHST- 2015/07/06 00:00 [received]
PHST- 2016/01/17 00:00 [accepted]
PHST- 2016/01/27 06:00 [entrez]
PHST- 2016/01/27 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
PHST- 2016/01/25 00:00 [pmc-release]
AID - 10.1186/s12985-016-0468-8 [pii]
AID - 468 [pii]
AID - 10.1186/s12985-016-0468-8 [doi]
PST - epublish
SO  - Virol J. 2016 Jan 25;13:13. doi: 10.1186/s12985-016-0468-8.