PMID- 26813475
OWN - NLM
STAT- MEDLINE
DCOM- 20170117
LR  - 20220317
IS  - 1525-1594 (Electronic)
IS  - 0160-564X (Linking)
VI  - 40
IP  - 6
DP  - 2016 Jun
TI  - Hypothermic Machine Perfusion Reduced Inflammatory Reaction by Downregulating the 
      Expression of Matrix Metalloproteinase 9 in a Reperfusion Model of Donation After 
      Cardiac Death.
PG  - E102-11
LID - 10.1111/aor.12658 [doi]
AB  - The exact mechanism by which hypothermic machine perfusion (HMP) improves the 
      graft quality in kidney transplantation of donation after cardiac death (DCD) 
      remains unclear. The aim of this study was to investigate the correlation between 
      the expression of matrix metalloproteinase 9 (MMP-9) and inflammatory reaction in 
      kidney ischemia-reperfusion (I/R) injury injury followed by cold storage (CS) or 
      HMP model of DCD. New Zealand white rabbit kidneys were subjected to 35 min of 
      warm ischemia and 1 h reperfusion, then preserved by either 1 h reperfusion 
      (sham-operated group), 4 h CS or 4 h HMP in vivo. Kidneys were reperfused 24 h 
      followed by further analysis. No treatment was given to rabbits in the normal 
      control group. The expression of MMP-9, nuclear factor-kappaB (NF-kappaB), and MMP-2 mRNA 
      were detected by real-time PCR (RT-PCR). MMP-9 was located by 
      immunohistochemistry and immunofluorescence methods. Tumor necrosis factor-alpha 
      (TNF-alpha), interleukin-6 (IL-6), myeloperoxidase (MPO), superoxide dismutase (SOD), 
      and malondialdehyde (MDA) were measured by kits for each groups. Compared with 
      the CS group, the expression of MMP-9 and NF-kappaB mRNA were downregulated in HMP 
      group (P < 0.05). In contrast, expression of MMP-2 mRNA had no statistical 
      significance between CS group and HMP group (P > 0.05). In normal control and 
      sham-operated groups, a low level of MMP-9 expression was detected in glomeruli. 
      However, positive signals of MMP-9 were mostly located in the tubulointerstitium 
      and the vascular wall of CS and HMP groups. Expression of TNF-alpha, IL-6, MDA, and 
      activity of MPO decreased while activity of SOD in the HMP group increased in 
      contrast to the CS group (P < 0.05). In conclusion, inflammatory cytokines 
      mediated MMP-9 expression through NF-kappaB band to MMP-9 promoter region, resulting 
      in renal injury. Therefore, HMP reduced inflammatory reaction by downregulating 
      the expression of MMP-9, which may be the mechanism of kidney protection in I/R 
      injury.
CI  - Copyright (c) 2016 International Center for Artificial Organs and Transplantation 
      and Wiley Periodicals, Inc.
FAU - Fu, Zhen
AU  - Fu Z
AD  - The 3rd Xiangya Hospital of Central South University, Research Center of National 
      Health Ministry on Transplantation Medicine Engineering and Technology, Changsha, 
      Hunan, China.
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Ye, Qifa
AU  - Ye Q
AD  - The 3rd Xiangya Hospital of Central South University, Research Center of National 
      Health Ministry on Transplantation Medicine Engineering and Technology, Changsha, 
      Hunan, China.
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Zhang, Yang
AU  - Zhang Y
AD  - The 3rd Xiangya Hospital of Central South University, Research Center of National 
      Health Ministry on Transplantation Medicine Engineering and Technology, Changsha, 
      Hunan, China.
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Zhong, Zibiao
AU  - Zhong Z
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Xiong, Yan
AU  - Xiong Y
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Wang, Yanfeng
AU  - Wang Y
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Hu, Long
AU  - Hu L
AD  - The 3rd Xiangya Hospital of Central South University, Research Center of National 
      Health Ministry on Transplantation Medicine Engineering and Technology, Changsha, 
      Hunan, China.
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Wang, Wei
AU  - Wang W
AD  - The 3rd Xiangya Hospital of Central South University, Research Center of National 
      Health Ministry on Transplantation Medicine Engineering and Technology, Changsha, 
      Hunan, China.
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Huang, Wei
AU  - Huang W
AD  - Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of 
      Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of 
      Medical Technology on Transplantation, Wuhan, Hubei, China.
FAU - Ko, Dicken Shiu-Chung
AU  - Ko DS
AD  - Department of Urology, Massachusetts General Hospital, Harvard Medical School, 
      Boston, MA, USA.
LA  - eng
PT  - Journal Article
DEP - 20160127
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Cold Temperature
MH  - Down-Regulation
MH  - Hypothermia, Induced/*methods
MH  - Inflammation/etiology/genetics/prevention & control
MH  - Kidney/*physiology
MH  - Male
MH  - Matrix Metalloproteinase 9/analysis/*genetics
MH  - Organ Preservation/*methods
MH  - Oxidative Stress
MH  - Perfusion/methods
MH  - RNA, Messenger/genetics
MH  - Rabbits
MH  - Renal Insufficiency/etiology/genetics/*prevention & control
MH  - Reperfusion Injury/etiology/genetics/*prevention & control
MH  - *Tissue and Organ Harvesting/methods
OTO - NOTNLM
OT  - Cold storage
OT  - Donation after cardiac death
OT  - Hypothermic machine perfusion
OT  - Ischemia-reperfusion
OT  - Matrix metalloproteinase-9
EDAT- 2016/01/28 06:00
MHDA- 2017/01/18 06:00
CRDT- 2016/01/28 06:00
PHST- 2016/01/28 06:00 [entrez]
PHST- 2016/01/28 06:00 [pubmed]
PHST- 2017/01/18 06:00 [medline]
AID - 10.1111/aor.12658 [doi]
PST - ppublish
SO  - Artif Organs. 2016 Jun;40(6):E102-11. doi: 10.1111/aor.12658. Epub 2016 Jan 27.