PMID- 26814020
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20170428
IS  - 1528-0691 (Electronic)
IS  - 1528-0691 (Linking)
VI  - 16
IP  - 2
DP  - 2016 Apr
TI  - Single-molecule Spectroscopy: Exploring Heterogeneity in Chemical and Biological 
      Systems.
PG  - 601-13
LID - 10.1002/tcr.201500214 [doi]
AB  - Many chemical and biological systems are heterogeneous in the molecular length 
      scale ( approximately  1 nm). Heterogeneity in many chemical systems and organized assemblies 
      may be monitored using single-molecule spectroscopy (SMS). In SMS, the size of 
      the focal spot (i.e., the smallest region to be probed) is nearly half of the 
      excitation wavelength (lambda/2, i.e., 200-375 nm) for visible light (400-750 nm). We 
      discuss how one can get spatial resolutions better than 200 nm using molecules as 
      nanometric probes. We show that polymer hydrogels, lipid vesicles, room 
      temperature ionic liquids (RTILs), and binary liquid mixtures exhibit such 
      heterogeneity. Another important observation is solute-dependent friction in 
      RTILs. In an RTIL, diffusion of an ionic solute is slower than that of a neutral 
      solute.
CI  - (c) 2016 The Chemical Society of Japan & Wiley-VCH Verlag GmbH & Co. KGaA, 
      Weinheim.
FAU - Ghosh, Shirsendu
AU  - Ghosh S
AD  - Department of Physical Chemistry, Indian Association for the Cultivation of 
      Science, 2A and 2B, Raja Subodh Chandra Mullick Rd, Jadavpur, Kolkata, West 
      Bengal, 700032, India.
FAU - Bhattacharyya, Kankan
AU  - Bhattacharyya K
AD  - Department of Physical Chemistry, Indian Association for the Cultivation of 
      Science, 2A and 2B, Raja Subodh Chandra Mullick Rd, Jadavpur, Kolkata, West 
      Bengal, 700032, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160127
PL  - United States
TA  - Chem Rec
JT  - Chemical record (New York, N.Y.)
JID - 101085550
RN  - 0 (Antibodies)
RN  - 0 (Hydrogels)
RN  - 0 (Ionic Liquids)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Liposomes)
RN  - 0 (Micelles)
RN  - 0 (Serum Albumin)
RN  - 0 (Surface-Active Agents)
RN  - 0 (Tubulin)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Antibodies/chemistry
MH  - Diffusion
MH  - Green Fluorescent Proteins/chemistry
MH  - Humans
MH  - Hydrogels
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Ionic Liquids/chemistry
MH  - Lipid Bilayers/chemistry
MH  - Liposomes/chemistry
MH  - Micelles
MH  - Protein Conformation
MH  - Serum Albumin/chemistry
MH  - Single Molecule Imaging/*methods
MH  - Surface-Active Agents/chemistry
MH  - Transition Temperature
MH  - Tubulin/chemistry
MH  - Viscosity
OTO - NOTNLM
OT  - binary mixture
OT  - conformational fluctuation of proteins
OT  - heterogeneity
OT  - ionic liquids
OT  - single-molecule studies
EDAT- 2016/01/28 06:00
MHDA- 2017/04/30 06:00
CRDT- 2016/01/28 06:00
PHST- 2015/07/22 00:00 [received]
PHST- 2016/01/28 06:00 [entrez]
PHST- 2016/01/28 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
AID - 10.1002/tcr.201500214 [doi]
PST - ppublish
SO  - Chem Rec. 2016 Apr;16(2):601-13. doi: 10.1002/tcr.201500214. Epub 2016 Jan 27.