PMID- 26815315 OWN - NLM STAT- MEDLINE DCOM- 20170410 LR - 20220317 IS - 1099-1263 (Electronic) IS - 0260-437X (Linking) VI - 36 IP - 9 DP - 2016 Sep TI - Evaluation of kidney injury biomarkers in rat amniotic fluid after gestational exposure to cadmium. PG - 1183-93 LID - 10.1002/jat.3286 [doi] AB - Cadmium is a well-characterized nephrotoxic agent that is also capable of accumulating and diffusing across the placenta; however, only a few studies have addressed its effects over fetal kidneys and none of them has used a panel of sensitive and specific biomarkers for the detection of kidney injury. The goal of this study was to determine cadmium renal effects in rat fetuses by the quantification of early kidney injury biomarkers. Pregnant Wistar rats were exposed by inhalation to an isotonic saline solution or to CdCl2 solution (DDel =1.48 mg Cd kg(-1) day(-1) ) during gestational days (GD) 8-20. On GD 21, dams were euthanized and samples obtained. Kidney injury biomarkers were quantified in amniotic fluid samples and fetal kidneys were microscopically evaluated to search for histological alterations. Our results showed that cadmium exposure significantly raised albumin, osteopontin, vascular endothelial growth factor and tissue inhibitor of metalloproteinases-1 levels in amniotic fluid, whereas it decreased creatinine. Clusterin, calbindin and IFN-inducible protein 10 did not show any change. Accordingly, histological findings showed tubular damage and precipitations in the renal pelvis. In conclusion, gestational exposure to cadmium induces structural alterations in fetal renal tissue that can be detected by some kidney injury biomarkers in amniotic fluid samples. Copyright (c) 2016 John Wiley & Sons, Ltd. CI - Copyright (c) 2016 John Wiley & Sons, Ltd. FAU - Jacobo-Estrada, Tania AU - Jacobo-Estrada T AD - Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, Col. San Pedro Zacatenco, CP 07360, Mexico, D.F., Mexico. FAU - Cardenas-Gonzalez, Mariana AU - Cardenas-Gonzalez M AD - Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, Col. San Pedro Zacatenco, CP 07360, Mexico, D.F., Mexico. FAU - Santoyo-Sanchez, Mitzi AU - Santoyo-Sanchez M AD - Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, Col. San Pedro Zacatenco, CP 07360, Mexico, D.F., Mexico. FAU - Parada-Cruz, Benjamin AU - Parada-Cruz B AD - Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, Col. San Pedro Zacatenco, CP 07360, Mexico, D.F., Mexico. FAU - Uria-Galicia, Esther AU - Uria-Galicia E AD - Departamento de Morfologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Prolongacion de Carpio y Plan de Ayala s/n, Col. Santo Tomas, CP 11340, Mexico, D.F., Mexico. FAU - Arreola-Mendoza, Laura AU - Arreola-Mendoza L AD - Departamento de Biociencias e Ingenieria, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Instituto Politecnico Nacional, 30 de Junio de 1520 s/n, Col. Barrio la Laguna Ticoman, CP 07340, Mexico, D.F., Mexico. FAU - Barbier, Olivier AU - Barbier O AD - Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, Col. San Pedro Zacatenco, CP 07360, Mexico, D.F., Mexico. LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20160127 PL - England TA - J Appl Toxicol JT - Journal of applied toxicology : JAT JID - 8109495 RN - 0 (Biomarkers) RN - 0 (Calb1 protein, rat) RN - 0 (Calbindin 1) RN - 0 (Chemokine CXCL10) RN - 0 (Clu protein, rat) RN - 0 (Clusterin) RN - 0 (Cxcl10 protein, rat) RN - 0 (Isoenzymes) RN - 0 (Spp1 protein, rat) RN - 0 (Vascular Endothelial Growth Factor A) RN - 00BH33GNGH (Cadmium) RN - 106441-73-0 (Osteopontin) RN - AYI8EX34EU (Creatinine) RN - EC 2.5.1.18 (Glutathione Transferase) RN - EC 2.5.1.18 (glutathione S-transferase alpha) RN - EC 3.4.- (Metalloproteases) SB - IM MH - Amniotic Fluid/drug effects/metabolism MH - Animals MH - Biomarkers/*metabolism MH - Cadmium/*toxicity MH - Calbindin 1/genetics/metabolism MH - Chemokine CXCL10/genetics/metabolism MH - Clusterin/genetics/metabolism MH - Creatinine/metabolism MH - Female MH - Fetus/drug effects/metabolism MH - Glutathione Transferase/genetics/metabolism MH - Isoenzymes/genetics/metabolism MH - Kidney/*drug effects/metabolism MH - Kidney Diseases/chemically induced/*metabolism MH - Metalloproteases/genetics/metabolism MH - Osteopontin/genetics/metabolism MH - Pregnancy MH - Rats MH - Rats, Wistar MH - Vascular Endothelial Growth Factor A/genetics/metabolism OTO - NOTNLM OT - TIMP-1 OT - VEGF OT - fetal nephrotoxicity OT - in utero exposure OT - metals OT - osteopontin EDAT- 2016/01/28 06:00 MHDA- 2017/04/11 06:00 CRDT- 2016/01/28 06:00 PHST- 2015/10/30 00:00 [received] PHST- 2015/12/11 00:00 [revised] PHST- 2015/12/12 00:00 [accepted] PHST- 2016/01/28 06:00 [entrez] PHST- 2016/01/28 06:00 [pubmed] PHST- 2017/04/11 06:00 [medline] AID - 10.1002/jat.3286 [doi] PST - ppublish SO - J Appl Toxicol. 2016 Sep;36(9):1183-93. doi: 10.1002/jat.3286. Epub 2016 Jan 27.