PMID- 2681538
OWN - NLM
STAT- MEDLINE
DCOM- 19891201
LR  - 20190724
IS  - 0022-510X (Print)
IS  - 0022-510X (Linking)
VI  - 92
IP  - 2-3
DP  - 1989 Sep
TI  - Neurotoxic activity in HTLV-I carrier lymphocyte culture.
PG  - 169-80
AB  - A close association between human T-lymphotropic virus type I (HTLV-I) infection 
      and a group of chronic myelopathies of unknown etiology has recently been 
      established and the name "HTLV-I associated myelopathy" (HAM) has been coined. 
      Although the mechanism of neural tissue damage in HAM remains virtually unknown, 
      several lines of evidence suggest the involvement of a soluble factor(s) 
      including cytokines and viral proteins in the disease process. In this study, we 
      examined cytopathic effects of the supernatants from 6 HTLV-I carrier human T 
      lymphocyte cell lines on 4 human and one murine neuroblastoma cell lines, and 2 
      human glioma cell lines. Among 6 lymphocyte cell culture supernatants, only 1 
      from MT-2 cell culture repeatedly exerted cytopathic effects on human 
      neuroblastoma cells, particularly on IMR-32 cells: marked retraction of neurites 
      leading to cellular clumping. This activity was neither abolished by treatment of 
      the medium at 80 degrees C for 30 min or by UV-irradiation, nor was it 
      neutralized by anti-HTLV-I antibodies. The MT-2 supernatant also induced mild 
      cytopathic changes in 2 other human neuroblastoma cell lines and 2 human glioma 
      cell lines. This activity was abolished by treatment of the medium at 80 degrees 
      C for 30 min but not at 56 degrees C for 30 min. Myelinated murine cerebellum 
      explants and other cell lines showed no morphological changes when incubated with 
      the MT-2 supernatant. In addition, the growth of THP-1 cells, a 
      monocyte/macrophage lineage cell line, was remarkably suppressed when maintained 
      in the MT-2 conditioned medium, accompanied by enhancement of phagocytic 
      activity. The THP-1 conditioned medium, on the other hand, suppressed tumor 
      necrosis factor (TNF) activity detected in the MT-2 culture. These observations 
      suggest that HTLV-I induced cytokines may directly act on neural cells, but their 
      action appears to be regulated by the intricate interactions of lymphocytic and 
      monocytic cells.
FAU - Terunuma, H
AU  - Terunuma H
AD  - Department of Neurological Sciences, Tohoku University School of Medicine, 
      Sendai, Japan.
FAU - Iwasaki, Y
AU  - Iwasaki Y
FAU - Tsukamoto, T
AU  - Tsukamoto T
FAU - Konno, H
AU  - Konno H
FAU - Yamamoto, T
AU  - Yamamoto T
FAU - Ohara, Y
AU  - Ohara Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neurol Sci
JT  - Journal of the neurological sciences
JID - 0375403
RN  - 0 (Biological Factors)
RN  - 0 (Cytokines)
RN  - 0 (Neurotoxins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Biological Factors/*metabolism/pharmacology
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cytokines
MH  - HTLV-I Infections/*metabolism
MH  - Humans
MH  - Mice
MH  - Neurotoxins/*metabolism/pharmacology
MH  - Tumor Cells, Cultured/*drug effects
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Viral Proteins/*metabolism/pharmacology
EDAT- 1989/09/01 00:00
MHDA- 1989/09/01 00:01
CRDT- 1989/09/01 00:00
PHST- 1989/09/01 00:00 [pubmed]
PHST- 1989/09/01 00:01 [medline]
PHST- 1989/09/01 00:00 [entrez]
AID - 0022-510X(89)90134-2 [pii]
AID - 10.1016/0022-510x(89)90134-2 [doi]
PST - ppublish
SO  - J Neurol Sci. 1989 Sep;92(2-3):169-80. doi: 10.1016/0022-510x(89)90134-2.