PMID- 26816641
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160128
LR  - 20201001
IS  - 2056-3418 (Print)
IS  - 2056-3426 (Electronic)
IS  - 2056-3426 (Linking)
VI  - 2
IP  - 3
DP  - 2015 Sep
TI  - The effects of icariine concentration on osteoclasts bone resorption induced by 
      titanium particles in vitro.
PG  - 197-202
LID - 10.1093/rb/rbv002 [doi]
AB  - In artificial joint replacement, osteoclast bone resorption induced by wear 
      debris of the implant is a main reason for aseptic loosening. To extend the life 
      of the prosthesis, detailed mechanisms of aseptic loosening and the ways to 
      prevent it should be explored. The aim of this study was to investigate the 
      in vitro effect of icariine on the bone resorption of osteoclasts induced by 
      titanium particles. Macrophage colony stimulating factor (M-CSF) and receptor 
      activator of NF-kB ligand (RANKL) were used to generate osteoclasts from RAW264.7 
      precursors. The proliferation of RAW264.7 precursors in the presence of different 
      doses of icariine was evaluated by MTT assay. The cells were treated with 
      titanium particles, titanium particles with icariine and culture medium only 
      (control), respectively. At 48 h after treatment, the expression level of 
      receptor activator of NF-kB (RANK) was detected by ELISA, and messenger RNA 
      (mRNA) levels of tartrate-resistant acid phosphatase (TRAP), matrix 
      metalloproteinase 9 (MMP-9), carbonic anhydrase II (CAII) and Cathepsin K (CtsK) 
      were determined by real-time polymerase chain reaction. Western blot was applied 
      to analyze the expression levels of TRAP, RANK and CtsK. In addition, bone chips 
      were cultured in the above conditions, and Toluidine blue staining was then 
      employed to calculate the number and area of resorption pits in the bone chips. 
      After treatment with icariine, expression level of RANK was significantly 
      decreased in the RAW264.7 cell that induced by titanium particle and its cultural 
      medium, mRNA and protein levels of TRAP, CAII, MMP-9 and CtsK were reduced as 
      well. In addition, the numbers of bone resorption pits and areas on bone slices 
      were both reduced by icariine challenging. Icariine could inhibit bone resorption 
      of osteoclast induced by titanium particle, and it might be used as a promising 
      drug for treating of aseptic loosening.
FAU - Zhang, Yiyuan
AU  - Zhang Y
AD  - Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen 
      University, Fuzhou 350007, China.
FAU - Lin, Yu
AU  - Lin Y
AD  - Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen 
      University, Fuzhou 350007, China.
FAU - Xiao, Lili
AU  - Xiao L
AD  - Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen 
      University, Fuzhou 350007, China.
FAU - Feng, Eryou
AU  - Feng E
AD  - Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen 
      University, Fuzhou 350007, China.
FAU - Wang, Wulian
AU  - Wang W
AD  - Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen 
      University, Fuzhou 350007, China.
FAU - Lin, Liqiong
AU  - Lin L
AD  - Joint Surgery Department, the Second Hospital of Fuzhou Affiliated to Xiamen 
      University, Fuzhou 350007, China.
LA  - eng
PT  - Journal Article
DEP - 20150810
PL  - England
TA  - Regen Biomater
JT  - Regenerative biomaterials
JID - 101652150
PMC - PMC4669013
OTO - NOTNLM
OT  - aseptic loosening
OT  - bone resorption
OT  - icariine
OT  - osteoclast
OT  - titanium particle
EDAT- 2016/01/28 06:00
MHDA- 2016/01/28 06:01
PMCR- 2015/08/10
CRDT- 2016/01/28 06:00
PHST- 2015/01/21 00:00 [received]
PHST- 2015/03/12 00:00 [revised]
PHST- 2015/03/14 00:00 [accepted]
PHST- 2016/01/28 06:00 [entrez]
PHST- 2016/01/28 06:00 [pubmed]
PHST- 2016/01/28 06:01 [medline]
PHST- 2015/08/10 00:00 [pmc-release]
AID - rbv002 [pii]
AID - 10.1093/rb/rbv002 [doi]
PST - ppublish
SO  - Regen Biomater. 2015 Sep;2(3):197-202. doi: 10.1093/rb/rbv002. Epub 2015 Aug 10.