PMID- 26818831
OWN - NLM
STAT- MEDLINE
DCOM- 20160822
LR  - 20181113
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 468
IP  - 4
DP  - 2016 Apr
TI  - Doing more with less: fluorescence in situ hybridization and gene sequencing 
      assays can be reliably performed on archival stained tumor tissue sections.
PG  - 451-61
LID - 10.1007/s00428-016-1906-0 [doi]
AB  - Little is known about molecular testing on tumor tissue retrieved from stained 
      sections, for which there may be a clinical need. We retrospectively analyzed 112 
      sections from 56 tumor patients using either fluorescence in situ hybridization 
      (FISH) with different probes (19 sections from 17 patients) or Sanger or targeted 
      next generation sequencing for detection of BRAF, EGFR, KRAS, C-KIT, and TP53 
      mutations (93 sections from 39 patients). Tumor tissue sections had been stained 
      by hematoxylin and eosin (H&E) (42 sections) or by immunohistochemistry for 
      cytoplasmic or nuclear/nuclear-cytoplasmic markers (70 sections) with a 
      peroxidase (P-IHC, with 3,3'-diaminobenzidine as chromogen) or alkaline 
      phosphatase label (AP-IHC, with Warp Red as chromogen). For FISH analysis, the 
      concordance rate between the original diagnosis and that obtained on H&E- or 
      P-IHC-stained tissue sections (AP-IHC was not on record for this set of patients) 
      was 95% (18 out of 19 tumor sections). Only one tumor sample, diffusely positive 
      for MLH1, did not yield any nuclear hybridization signal. For sequencing 
      analysis, the concordance rate was 100% on negative P-IHC and positive 
      AP-IHC-stained sections, regardless of the subcellular localization of the 
      reaction product. Mutations were detected in only 52% of cases expressing 
      nuclear/nuclear-cytoplasmic markers, regardless of the sequencing technology used 
      (p = 0.0002). In conclusion, stained sections may be a valuable resource for FISH 
      or sequencing analysis, but on cases expressing nuclear markers sequencing 
      results need to be interpreted cautiously.
FAU - Pelosi, Giuseppe
AU  - Pelosi G
AUID- ORCID: 0000-0003-4725-4692
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy. 
      giuseppe.pelosi@unimi.it.
AD  - Department of Biomedical and Clinical Sciences "Luigi Sacco", Universita degli 
      Studi, Milan, Italy. giuseppe.pelosi@unimi.it.
FAU - Perrone, Federica
AU  - Perrone F
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Tamborini, Elena
AU  - Tamborini E
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Fabbri, Alessandra
AU  - Fabbri A
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Testi, Maria Adele
AU  - Testi MA
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Busico, Adele
AU  - Busico A
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Settanni, Giulio
AU  - Settanni G
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Picciani, Benedetta
AU  - Picciani B
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Bovio, Enrica
AU  - Bovio E
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Sonzogni, Angelica
AU  - Sonzogni A
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Valeri, Barbara
AU  - Valeri B
AD  - Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto 
      Nazionale Tumori, Via Giacomo Venezian, 1, 20133, Milano, Italy.
FAU - Garassino, Marina
AU  - Garassino M
AD  - Department of Medical Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 
      Milan, Italy.
FAU - De Braud, Filippo
AU  - De Braud F
AD  - Department of Medical Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 
      Milan, Italy.
FAU - Pastorino, Ugo
AU  - Pastorino U
AD  - Division of Thoracic Surgery, Fondazione IRCCS Istituto Nazionale dei Tumori, 
      Milan, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160127
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*analysis
MH  - DNA Mutational Analysis/*methods
MH  - Feasibility Studies
MH  - Female
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Neoplasms/*genetics
MH  - Staining and Labeling
MH  - Young Adult
OTO - NOTNLM
OT  - Direct sequencing
OT  - Fish
OT  - Immunohistochemistry
OT  - Next generation sequencing
EDAT- 2016/01/29 06:00
MHDA- 2016/08/23 06:00
CRDT- 2016/01/29 06:00
PHST- 2015/08/13 00:00 [received]
PHST- 2016/01/13 00:00 [accepted]
PHST- 2015/12/20 00:00 [revised]
PHST- 2016/01/29 06:00 [entrez]
PHST- 2016/01/29 06:00 [pubmed]
PHST- 2016/08/23 06:00 [medline]
AID - 10.1007/s00428-016-1906-0 [pii]
AID - 10.1007/s00428-016-1906-0 [doi]
PST - ppublish
SO  - Virchows Arch. 2016 Apr;468(4):451-61. doi: 10.1007/s00428-016-1906-0. Epub 2016 
      Jan 27.