PMID- 26818976
OWN - NLM
STAT- MEDLINE
DCOM- 20170106
LR  - 20231213
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Jan 28
TI  - miR-23a, miR-146a and miR-301a confer predisposition to Vogt-Koyanagi-Harada 
      syndrome but not to Behcet's disease.
PG  - 20057
LID - 10.1038/srep20057 [doi]
LID - 20057
AB  - Ninety-eight miRNAs are involved in the immune response. However, the genetic 
      roles of these miRNAs remain unclear in Behcet's disease (BD) and 
      Vogt-Koyanagi-Harada (VKH) syndrome. This study aimed to explore the association 
      and functional roles of copy number variants (CNV) in several miRNAs with BD and 
      VKH syndrome. Genotyping of CNVs was examined by TaqMan PCR. The expression of 
      miR-23a, transfection efficiency and cytokine production were measured by 
      real-time PCR, flow cytometry or ELISA. First, replication and combined studies 
      for miR-23a, miR-146a and miR-301a demonstrated a similar association with VKH 
      syndrome (Combined: P = 5.53 x 10(-8); P = 8.43 x 10(-31); P = 9.23 x 10(-8), 
      respectively). No association of CNVs of the above mentioned miRNAs was observed 
      in BD patients. mRNA expression of miR-23a showed a positive association with its 
      copy numbers. Additionally, individuals with high copy number of miR-23a show an 
      increased production of interleukin-6 (IL-6), but not IL-8 and monocyte 
      chemoattractant protein-1 (MCP-1) by stimulated PBMCs. miR-23a transfected 
      ARPE-19 cells modulated the production of IL-6 and IL-8, but not MCP-1. Our 
      results suggest that CNVs of miR-146a, miR-23a and miR-301a confer susceptibility 
      to VKH syndrome, but not to BD. The contribution of miR-23a to VKH syndrome may 
      be mediated by increasing the production of IL-6.
FAU - Hou, Shengping
AU  - Hou S
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Eye Institute and Chongqing Key Laboratory of Ophthalmology, Chongqing, 
      China.
FAU - Ye, Zi
AU  - Ye Z
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Eye Institute and Chongqing Key Laboratory of Ophthalmology, Chongqing, 
      China.
FAU - Liao, Dan
AU  - Liao D
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Eye Institute and Chongqing Key Laboratory of Ophthalmology, Chongqing, 
      China.
FAU - Bai, Lin
AU  - Bai L
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Eye Institute and Chongqing Key Laboratory of Ophthalmology, Chongqing, 
      China.
FAU - Liu, Yunjia
AU  - Liu Y
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Eye Institute and Chongqing Key Laboratory of Ophthalmology, Chongqing, 
      China.
FAU - Zhang, Jun
AU  - Zhang J
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Eye Institute and Chongqing Key Laboratory of Ophthalmology, Chongqing, 
      China.
FAU - Kijlstra, Aize
AU  - Kijlstra A
AD  - University Eye Clinic Maastricht, Maastricht, The Netherlands.
FAU - Yang, Peizeng
AU  - Yang P
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Eye Institute and Chongqing Key Laboratory of Ophthalmology, Chongqing, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160128
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cytokines)
RN  - 0 (MIRN146 microRNA, human)
RN  - 0 (MIRN23a microRNA, human)
RN  - 0 (MIRN301A microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adult
MH  - Behcet Syndrome/diagnosis/*genetics/metabolism
MH  - Case-Control Studies
MH  - Cell Line
MH  - Cytokines/genetics/metabolism
MH  - DNA Copy Number Variations
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Gene Dosage
MH  - Gene Expression
MH  - *Genetic Association Studies
MH  - *Genetic Predisposition to Disease
MH  - Humans
MH  - Leukocytes, Mononuclear/metabolism
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Retinal Pigment Epithelium/metabolism
MH  - Uveomeningoencephalitic Syndrome/diagnosis/*genetics/metabolism
MH  - Young Adult
PMC - PMC4730241
EDAT- 2016/01/29 06:00
MHDA- 2017/01/07 06:00
PMCR- 2016/01/28
CRDT- 2016/01/29 06:00
PHST- 2015/06/16 00:00 [received]
PHST- 2015/09/09 00:00 [accepted]
PHST- 2016/01/29 06:00 [entrez]
PHST- 2016/01/29 06:00 [pubmed]
PHST- 2017/01/07 06:00 [medline]
PHST- 2016/01/28 00:00 [pmc-release]
AID - srep20057 [pii]
AID - 10.1038/srep20057 [doi]
PST - epublish
SO  - Sci Rep. 2016 Jan 28;6:20057. doi: 10.1038/srep20057.