PMID- 26820673
OWN - NLM
STAT- MEDLINE
DCOM- 20180213
LR  - 20220409
IS  - 1559-1182 (Electronic)
IS  - 0893-7648 (Linking)
VI  - 54
IP  - 2
DP  - 2017 Mar
TI  - Elevated Serum Brain-Derived Neurotrophic Factor (BDNF) but not BDNF Gene 
      Val66Met Polymorphism Is Associated with Autism Spectrum Disorders.
PG  - 1167-1172
LID - 10.1007/s12035-016-9721-9 [doi]
AB  - The aim of our study was to illuminate the potential role of brain-derived 
      neurotrophic factor (BDNF) in autism spectrum disorder (ASD). We measured the 
      circulating levels of BDNF in serum and BDNF gene (Val66Met) polymorphisms, in 
      which two indicators were then compared between ASD and normal controls. A total 
      of 82 drug-naive ASD children and 82 age- and gender-matched normal controls were 
      enrolled in the study. Their serum BDNF levels were detected by the ELISA. BDNF 
      Val66Met polymorphism genotyping was conducted as according to the laboratory's 
      standard protocol in laboratory. The ASD severity assessment was mainly 
      determined by the score of the Childhood Autism Rating Scale (CARS). ELISA assay 
      showed that the mean serum BDNF level of children with ASD was significantly 
      (P < 0.0001) higher than that of the control cases (17.75 +/- 5.43 vs. 
      11.49 +/- 2.85 ng/ml; t = 9.236). Besides, the serum BDNF levels and CARS scores 
      (P < 0.0001) were positively related. And, the BDNF genotyping results showed 
      that there was no difference between the ASD cases and the control. Among the 
      children with ASD, the mean serum BDNF level of Met/Met group was lower than 
      other groups. According to the ROC curve generated from our clinical data, the 
      optimal cutoff value of serum BDNF levels, an indicator for diagnosis of ASD, was 
      projected to be 12.50 ng/ml. Thus, it yielded a corresponding sensitivity of 
      81.7 % and the specificity of 66.9 %. Accordingly, area value under the curve was 
      0.836 (95 % CI, 0.774-0.897); the positive predictive value (PPV) and the 
      negative predictive value (NPV) were 70.1 and 79.1 %, respectively. These results 
      suggested that rather than Val66Met polymorphism, BDNF was more possible to 
      impact the pathogenesis of ASD.
FAU - Meng, Wei-Dong
AU  - Meng WD
AD  - Department of Laboratory Medicine, Liaocheng People's Hospital, No. 67, Dongchang 
      West Road, Liaocheng, 252000, China.
FAU - Sun, Shao-Jun
AU  - Sun SJ
AD  - Department of Laboratory Medicine, Liaocheng People's Hospital, No. 67, Dongchang 
      West Road, Liaocheng, 252000, China. wangyws72@163.com.
FAU - Yang, Jie
AU  - Yang J
AD  - Department of Pharmacy, Liaocheng Herbalist Hospital, Liaocheng, China.
FAU - Chu, Rui-Xue
AU  - Chu RX
AD  - Department of Laboratory Medicine, Liaocheng People's Hospital, No. 67, Dongchang 
      West Road, Liaocheng, 252000, China.
FAU - Tu, Wenjun
AU  - Tu W
AD  - Institute of Radiation Medicine, Academy of Medical Science and Peking Union 
      Medical College, No. 238, Baiti Road, Tianjin, 300192, China.
FAU - Liu, Qiang
AU  - Liu Q
AD  - Institute of Radiation Medicine, Academy of Medical Science and Peking Union 
      Medical College, No. 238, Baiti Road, Tianjin, 300192, China. 
      liuqiang@irm-cams.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20160128
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - AE28F7PNPL (Methionine)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - Autism Spectrum Disorder/*blood/diagnosis/*genetics
MH  - Biomarkers/blood
MH  - Brain-Derived Neurotrophic Factor/*blood/*genetics
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Genetic Association Studies/*methods
MH  - Humans
MH  - Male
MH  - Methionine/genetics
MH  - Polymorphism, Genetic/*physiology
MH  - Valine/genetics
OTO - NOTNLM
OT  - Autism spectrum disorders
OT  - BDNF Val66Met polymorphism
OT  - Biomarker
OT  - Brain-derived neurotrophic factor
OT  - Chinese
EDAT- 2016/01/29 06:00
MHDA- 2018/02/14 06:00
CRDT- 2016/01/29 06:00
PHST- 2015/11/22 00:00 [received]
PHST- 2016/01/12 00:00 [accepted]
PHST- 2016/01/29 06:00 [pubmed]
PHST- 2018/02/14 06:00 [medline]
PHST- 2016/01/29 06:00 [entrez]
AID - 10.1007/s12035-016-9721-9 [pii]
AID - 10.1007/s12035-016-9721-9 [doi]
PST - ppublish
SO  - Mol Neurobiol. 2017 Mar;54(2):1167-1172. doi: 10.1007/s12035-016-9721-9. Epub 
      2016 Jan 28.