PMID- 26820972
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160916
LR  - 20181113
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 68
IP  - 5
DP  - 2016 Oct
TI  - Generation and characterization of human cardiac resident and non-resident 
      mesenchymal stem cell.
PG  - 2061-73
LID - 10.1007/s10616-016-9946-5 [doi]
AB  - Despite the surgical and other insertional interventions, the complete 
      recuperation of myocardial disorders is still elusive due to the insufficiency of 
      functioning myocardiocytes. Thus, the use of stem cells to regenerate the 
      affected region of heart becomes a prime important. In line with this human 
      umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have gained considerable 
      interest due to their potential use for mesodermal cell based replacement therapy 
      and tissue engineering. Since MSCs are harvested from various organs and 
      anatomical locations of same organism, thus the cardiac regenerative potential of 
      human cardiac-derived MSCs (hC-MSCs) and human umbilical cord Wharton's Jelly 
      derived MSC (hUC-MSCs) were tested concurrently. At in vitro culture, both 
      hUC-MSCs and hC-MSCs assumed spindle shape morphology with expression of typical 
      MSC markers namely CD105, CD73, CD90 and CD44. Although, hUC-MSCs and hC-MSCs are 
      identical in term of morphology and immunophenotype, yet hUC-MSCs harbored a 
      higher cell growth as compared to the hC-MSCs. The inherent cardiac regenerative 
      potential of both cells were further investigated with mRNA expression of ion 
      channels. The RT-PCR results demonstrated that both MSCs were expressing a 
      notable level of delayed rectifier-like K(+) current (I KDR ) ion channel, yet 
      the relative expression level was considerably varied between hUC-MSCs and 
      hC-MSCs that Kv1.1(39 +/- 0.6 vs 31 +/- 0.8), Kv2.1 (6 +/- 0.2 vs 21 +/- 0.12), Kv1.5 
      (7.4 +/- 0.1 vs 6.8 +/- 0.06) and Kv7.3 (27 +/- 0.8 vs 13.8 +/- 0.6). Similarly, the 
      Ca2(+)-activated K(+) current (I KCa ) channel encoding gene, transient outward 
      K(+) current (I to ) and TTX-sensitive transient inward sodium current (I Na.TTX 
      ) encoding gene (Kv4.2, Kv4.3 and hNE-Na) expressions were detected in both 
      groups as well. Despite the morphological and phenotypical similarity, the 
      present study also confirms the existence of multiple functional ion channel 
      currents IKDR, IKCa, Ito, and INa.TTX in undifferentiated hUC-MSCs as of hC-MSCs. 
      Thus, the hUC-MSCs can be exploited as a potential candidate for future cardiac 
      regeneration.
FAU - Subramani, Baskar
AU  - Subramani B
AD  - Nichi-Asia Life Sdn Bhd., 47810, Petaling Jaya, Selangor, Malaysia.
AD  - Bharathiyar University, Coimbatore, Tamil Nadu, India.
FAU - Subbannagounder, Sellamuthu
AU  - Subbannagounder S
AD  - Nichi-Asia Life Sdn Bhd., 47810, Petaling Jaya, Selangor, Malaysia.
FAU - Palanivel, Sekar
AU  - Palanivel S
AD  - Departments of Zoology, Government Arts College (Autonomous), Salem, 636007, 
      Tamil Nadu, India.
FAU - Ramanathanpullai, Chithra
AU  - Ramanathanpullai C
AD  - Nichi-Asia Life Sdn Bhd., 47810, Petaling Jaya, Selangor, Malaysia.
FAU - Sivalingam, Sivakumar
AU  - Sivalingam S
AD  - Cardiothoracic Surgery Unit, National Heart Institute, 50400, Kuala Lumpur, 
      Malaysia.
FAU - Yakub, Azhari
AU  - Yakub A
AD  - Cardiothoracic Surgery Unit, National Heart Institute, 50400, Kuala Lumpur, 
      Malaysia.
FAU - SadanandaRao, Manjunath
AU  - SadanandaRao M
AD  - Micro Therapeutic Research Labs Pvt. Ltd, Chennai, Tamil Nadu, India.
FAU - Seenichamy, Arivudainambi
AU  - Seenichamy A
AD  - Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 
      Serdang, Malaysia.
FAU - Pandurangan, Ashok Kumar
AU  - Pandurangan AK
AD  - Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, 
      Kuala Lumpur, Malaysia.
FAU - Tan, Jun Jie
AU  - Tan JJ
AD  - Regeneration Medicine Cluster, Advanced Medicine and Dental Institute, Universiti 
      Sains Malaysia, George Town, Pulau Pinang, Malaysia.
FAU - Ramasamy, Rajesh
AU  - Ramasamy R
AD  - Stem Cell and Immunity Group, Immunology Laboratory Unit, Department Of 
      Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 
      43400, Serdang, Selangor, Malaysia. rajesh@upm.edu.my.
AD  - Stem Cell Research Laboratory, Genetic and Regenerative Medicine Research Center, 
      Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, 
      Serdang, Selangor, Malaysia. rajesh@upm.edu.my.
LA  - eng
PT  - Journal Article
DEP - 20160128
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC5023579
OTO - NOTNLM
OT  - Cardiac resident stem cell and umbilical cord stem cell
OT  - Electrophysiology
OT  - Mesenchymal stem cell
COIS- The authors express no conflicts of interest towards the publication of this 
      paper.
EDAT- 2016/01/29 06:00
MHDA- 2016/01/29 06:01
PMCR- 2017/10/01
CRDT- 2016/01/29 06:00
PHST- 2015/10/02 00:00 [received]
PHST- 2016/01/14 00:00 [accepted]
PHST- 2016/01/29 06:00 [entrez]
PHST- 2016/01/29 06:00 [pubmed]
PHST- 2016/01/29 06:01 [medline]
PHST- 2017/10/01 00:00 [pmc-release]
AID - 10.1007/s10616-016-9946-5 [pii]
AID - 9946 [pii]
AID - 10.1007/s10616-016-9946-5 [doi]
PST - ppublish
SO  - Cytotechnology. 2016 Oct;68(5):2061-73. doi: 10.1007/s10616-016-9946-5. Epub 2016 
      Jan 28.