PMID- 26822433
OWN - NLM
STAT- MEDLINE
DCOM- 20161226
LR  - 20210614
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 52
IP  - 4
DP  - 2016 Apr
TI  - MicroRNA-29 regulates high-glucose-induced apoptosis in human retinal pigment 
      epithelial cells through PTEN.
PG  - 419-26
LID - 10.1007/s11626-015-9990-z [doi]
AB  - Hyperglycemia or high-glucose (HG)-induced apoptosis in human retinal pigment 
      epithelial (RPE) cells is a characteristic process in diabetic retinopathy. In 
      our study, we examined whether microRNA-29 (miR-29) may regulate HG-induced RPE 
      cell apoptosis. Human RPE cell line, ARPE-19 cells, was treated with various high 
      concentration of glucose in vitro. HG-induced RPE cell apoptosis was examined by 
      terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) 
      assay and miR-29 gene expression by quantitative RT-PCR (qRT-PCR). miR-29 was 
      then downregulated in RPE cells, and its effect on HG-induced apoptosis was 
      examined by TUNEL assay and western blot assay on caspase-7 protein. Association 
      of miR-29 on its downstream target, PTEN, in HG-induced RPE cell apoptosis was 
      evaluated by dual-luciferase assay and qRT-PCR. PTEN was silenced in RPE cells. 
      The effects of PTEN downregulation on miR-29-mediated HG-induced RPE cell 
      apoptosis were also examined by TUNEL and western blot assays. HG induced 
      significant apoptosis in RPE cells in a dose-dependent manner. miR-29 was 
      upregulated by HG in RPE cells. miR-29 downregulation protected HG-induced 
      apoptosis and reduced the production of caspase-7 protein in RPE cells. PTEN was 
      shown to be directly downregulated by HG and then upregulated by miR-29 
      downregulation in RPE cells. Small interfering RNA (siRNA)-mediated PTEN 
      downregulation reversed the protective effect of miR-29 downregulation on 
      HG-induced RPE cell apoptosis. This study demonstrates that miR-29, through 
      inverse association of PTEN, plays an important role in the process of HG-induced 
      apoptosis in RPE cells.
FAU - Lin, Xiaohui
AU  - Lin X
AD  - Department of Ophthalmology, The Second Affiliated Hospital of Chongqing Medical 
      University, 76 Linjiang Road, Chongqing, 400010, China.
FAU - Zhou, Xiyuan
AU  - Zhou X
AD  - Department of Ophthalmology, The Second Affiliated Hospital of Chongqing Medical 
      University, 76 Linjiang Road, Chongqing, 400010, China. xiyuan.zhou@aol.com.
FAU - Liu, Danning
AU  - Liu D
AD  - Department of Ophthalmology, The Second Affiliated Hospital of Chongqing Medical 
      University, 76 Linjiang Road, Chongqing, 400010, China.
FAU - Yun, Lixia
AU  - Yun L
AD  - Department of Ophthalmology, The Inner Mongolia Autonomous Region People's 
      Hospital, 20 Zhaowuda Road, Hohhot, 010010, China.
FAU - Zhang, Lina
AU  - Zhang L
AD  - Department of Ophthalmology, The Inner Mongolia Autonomous Region People's 
      Hospital, 20 Zhaowuda Road, Hohhot, 010010, China.
FAU - Chen, Xiaohai
AU  - Chen X
AD  - Department of Ophthalmology, The Inner Mongolia Autonomous Region People's 
      Hospital, 20 Zhaowuda Road, Hohhot, 010010, China.
FAU - Chai, Qinghe
AU  - Chai Q
AD  - Department of Ophthalmology, The Inner Mongolia Autonomous Region People's 
      Hospital, 20 Zhaowuda Road, Hohhot, 010010, China.
FAU - Li, Langen
AU  - Li L
AD  - Department of Ophthalmology, The Inner Mongolia Autonomous Region People's 
      Hospital, 20 Zhaowuda Road, Hohhot, 010010, China.
LA  - eng
PT  - Journal Article
DEP - 20160128
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (MIRN29a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
RN  - EC 3.4.22.- (Caspases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Base Sequence
MH  - Caspases/metabolism
MH  - Cell Line
MH  - Cytoprotection/drug effects
MH  - Down-Regulation/drug effects
MH  - Epithelial Cells/drug effects/*metabolism
MH  - Glucose/*pharmacology
MH  - Humans
MH  - MicroRNAs/genetics/*metabolism
MH  - PTEN Phosphohydrolase/antagonists & inhibitors/*metabolism
MH  - Retinal Pigment Epithelium/*cytology
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - Apoptosis
OT  - High glucose
OT  - PTEN
OT  - Retinal pigment epithelial cells
OT  - miR-29
EDAT- 2016/01/30 06:00
MHDA- 2016/12/27 06:00
CRDT- 2016/01/30 06:00
PHST- 2015/10/29 00:00 [received]
PHST- 2015/12/09 00:00 [accepted]
PHST- 2016/01/30 06:00 [entrez]
PHST- 2016/01/30 06:00 [pubmed]
PHST- 2016/12/27 06:00 [medline]
AID - 10.1007/s11626-015-9990-z [pii]
AID - 10.1007/s11626-015-9990-z [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2016 Apr;52(4):419-26. doi: 
      10.1007/s11626-015-9990-z. Epub 2016 Jan 28.