PMID- 26822763
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20240104
IS  - 1756-9966 (Electronic)
IS  - 0392-9078 (Print)
IS  - 0392-9078 (Linking)
VI  - 35
DP  - 2016 Jan 29
TI  - Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through 
      regulating miR-107/CDK6 pathway.
PG  - 22
LID - 10.1186/s13046-016-0297-z [doi]
LID - 22
AB  - BACKGROUND: Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) 
      plays key role in the progression of some human cancers. However, the role of 
      NEAT1 in human laryngeal squamous cell cancer (LSCC) is still unknown. We 
      therefore investigated the expression and function of NEAT1 in LSCC. METHODS: 
      NEAT1 level in LSCC and adjacent non-neoplastic tissues were detected by qRT-PCR. 
      NEAT1 was knockdown in LSCC cells and cell proliferation, apoptosis and cell 
      cycle were examined. The growth of xenografts with NEAT1 knockdown LSCC cells was 
      analyzed. RESULTS: NEAT1 level was significantly higher in LSCC than in 
      corresponding adjacent non-neoplastic tissues, and patients with neck nodal 
      metastasis or advanced clinical stage had higher NEAT1 expression. Moreover, 
      siRNA mediated NEAT1 knockdown significantly inhibited the proliferation and 
      induced apoptosis and cell cycle arrest at G1 phase in LSCC cells. The growth of 
      LSCC xenografts was significantly suppressed by the injection of NEAT1 siRNA 
      lentivirus. Furthermore, NEAT1 regulated CDK6 expression in LSCC cells which was 
      mediated by miR-107. CONCLUSION: NEAT1 plays an oncogenic role in the 
      tumorigenesis of LSCC and may serve as a potential target for therapeutic 
      intervention.
FAU - Wang, Peng
AU  - Wang P
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. 350239591@qq.com.
FAU - Wu, Tianyi
AU  - Wu T
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. tianshiyi.hot@163.com.
FAU - Zhou, Han
AU  - Zhou H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. zhou15153887577@163.com.
FAU - Jin, Qianqian
AU  - Jin Q
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. 826108898@qq.com.
FAU - He, Guoqing
AU  - He G
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. drhgq@hotmail.com.
FAU - Yu, Haoyang
AU  - Yu H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. haoyang1028@yeah.net.
FAU - Xuan, Lijia
AU  - Xuan L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. xuanlijia1992@126.com.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. 546181026@qq.com.
FAU - Tian, Linli
AU  - Tian L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. 76202920@qq.com.
FAU - Sun, Yanan
AU  - Sun Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. hydeyebh@sina.com.
FAU - Liu, Ming
AU  - Liu M
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246, Baojian Road, Harbin, 150086, 
      China. liuming002@outlook.com.
FAU - Qu, Lingmei
AU  - Qu L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Fifth Affiliated 
      Hospital, Harbin Medical University, No. 213, Jianshe Road, Daqing, China. 
      qlm8977976@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160129
PL  - England
TA  - J Exp Clin Cancer Res
JT  - Journal of experimental & clinical cancer research : CR
JID - 8308647
RN  - 0 (MIRN107 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NEAT1 long non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.7.11.22 (CDK6 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 6)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cyclin-Dependent Kinase 6/*genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Neoplasm Transplantation
MH  - RNA, Long Noncoding/*genetics
MH  - Signal Transduction
PMC - PMC4731996
EDAT- 2016/01/30 06:00
MHDA- 2016/10/14 06:00
PMCR- 2016/01/29
CRDT- 2016/01/30 06:00
PHST- 2015/10/27 00:00 [received]
PHST- 2016/01/21 00:00 [accepted]
PHST- 2016/01/30 06:00 [entrez]
PHST- 2016/01/30 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
PHST- 2016/01/29 00:00 [pmc-release]
AID - 10.1186/s13046-016-0297-z [pii]
AID - 297 [pii]
AID - 10.1186/s13046-016-0297-z [doi]
PST - epublish
SO  - J Exp Clin Cancer Res. 2016 Jan 29;35:22. doi: 10.1186/s13046-016-0297-z.