PMID- 26823701
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20190221
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 8
IP  - 11
DP  - 2015
TI  - Zinc finger protein x-linked (ZFX) contributes to patient prognosis, cell 
      proliferation and apoptosis in human laryngeal squamous cell carcinoma.
PG  - 13886-99
AB  - Zinc finger protein, X-linked (ZFX) gene locus on the human X chromosome is 
      structurally similar to the zinc finger protein, Y-linked gene. Its role in human 
      laryngeal squamous cell carcinoma (LSCC) is still not clearly defined. This study 
      was focused on investigating the role of zinc-finger protein X-linked (ZFX) in 
      human LSCC. Expression levels of ZFX were examined in LSCC tissues, corresponding 
      adjacent non-tumoral tissues and vocal leukoplakia tissues by 
      immunohistochemistry (IHC). The association with the expression level of ZFX and 
      LSCC clincopathological parameters was analyzed. The prognostic value of ZFX 
      expression was also analyzed. Lentivirus-mediated RNA interference was applied to 
      silence ZFX expression and the effects of ZFX knockdown on the growth of human 
      LSCC primary cells was investigated. Overexpression of ZFX was found in LSCC 
      tissues. The expression of ZFX was associated with the clinical stage of LSCC. 
      Patients with higher level of ZFX experienced a poorer prognosis compared to 
      those with lower level of ZFX. Knockdown of ZFX inhibited cell proliferation, 
      colony formation and migration of LSCC primary cells. Moreover, ZFX silencing 
      induced cell apoptosis. These results provide the convincing evidence for the 
      first time that ZFX plays an important role in LSCC development and could be a 
      potential therapeutic target or prognostic predictor for LSCC.
FAU - Yang, Fan
AU  - Yang F
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, 
      Capital Medical University Beijing 100029, China.
FAU - Ma, Hongzhi
AU  - Ma H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical UniversityBeijing 100730, China; Key Laboratory of 
      Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical 
      UniversityBeijing 100730, China.
FAU - Feng, Ling
AU  - Feng L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical UniversityBeijing 100730, China; Key Laboratory of 
      Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical 
      UniversityBeijing 100730, China.
FAU - Lian, Meng
AU  - Lian M
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University Beijing 100730, China.
FAU - Wang, Ru
AU  - Wang R
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University Beijing 100730, China.
FAU - Fan, Erzhong
AU  - Fan E
AD  - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of 
      Education, Capital Medical University Beijing 100730, China.
FAU - Fang, Jugao
AU  - Fang J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical UniversityBeijing 100730, China; Key Laboratory of 
      Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Capital Medical 
      UniversityBeijing 100730, China; Beijing Key Laboratory of Head and Neck 
      Molecular Diagnostic PathologyBeijing 100730, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151101
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (zinc finger protein, X-linked)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Apoptosis
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/mortality/pathology/surgery
MH  - Cell Movement
MH  - *Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - HEK293 Cells
MH  - Head and Neck Neoplasms/genetics/*metabolism/mortality/pathology/surgery
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Kruppel-Like Transcription Factors/genetics/*metabolism
MH  - Laryngeal Neoplasms/genetics/*metabolism/mortality/pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Proportional Hazards Models
MH  - RNA Interference
MH  - Signal Transduction
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Time Factors
MH  - Transfection
MH  - Treatment Outcome
MH  - Tumor Cells, Cultured
PMC - PMC4713487
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma (LSCC)
OT  - apoptosis
OT  - prognosis
OT  - proliferation
OT  - zinc finger protein x-linked (ZFX)
EDAT- 2016/01/30 06:00
MHDA- 2016/11/01 06:00
PMCR- 2015/11/01
CRDT- 2016/01/30 06:00
PHST- 2015/05/19 00:00 [received]
PHST- 2015/06/27 00:00 [accepted]
PHST- 2016/01/30 06:00 [entrez]
PHST- 2016/01/30 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2015 Nov 1;8(11):13886-99. eCollection 2015.