PMID- 26825344
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 408
IP  - 9
DP  - 2016 Mar
TI  - Gas chromatography-mass spectrometry and Raman imaging measurement of squalene 
      content and distribution in human hair.
PG  - 2357-62
LID - 10.1007/s00216-016-9335-0 [doi]
AB  - A sensitive and specific gas chromatography-mass spectrometry (GC-MS) method was 
      developed and validated for the measurement of the squalene content from root to 
      tip, in both Chinese black virgin and bleached hair. Deuterated squalene was used 
      as the internal standard. For quantification, selective ion monitoring (SIM) at 
      m/z 410.0 and 347.0 were monitored for squalene and deuterated squalene, 
      respectively. Different methods for the extraction of squalene from ex vivo human 
      hair were compared including organic solvent extraction and acid/alkali 
      hydrolysis. The best extraction efficiency was obtained by using a mixed solvent 
      consisting of chloroform:methanol = 2:1 (v:v). The linear range of squalene ran 
      from 1.0 to 50.0 mug mL(-1). The limit of detection (LOD) was 0.10 mug mL(-1) 
      (corresponding to 0.005 mg g(-1) in human hair), which enabled quantification of 
      squalene in human hair at very low level. The recovery of squalene was 
      96.4 +/- 1.46% (n = 3). Using the above-mentioned mixed solvent extraction, 
      squalene content in human hair was successfully quantified from root to tip. 
      Meanwhile, a Raman imaging method was developed to visualize the squalene 
      distribution in Chinese white virgin hair from cuticle to medulla.
FAU - Wu, Yan
AU  - Wu Y
AD  - Unilever R&D Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, 
      Shanghai, 200335, China. yan.wu@unilever.com.
AD  - Analytical-Chemistry Group, Van't Hoff Institute for Molecular Sciences, 
      University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands. 
      yan.wu@unilever.com.
FAU - Chen, Guoqiang
AU  - Chen G
AD  - Unilever R&D Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, 
      Shanghai, 200335, China.
AD  - Analytical-Chemistry Group, Van't Hoff Institute for Molecular Sciences, 
      University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.
FAU - Ji, Chengdong
AU  - Ji C
AD  - Unilever R&D Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, 
      Shanghai, 200335, China.
FAU - Hoptroff, Michael
AU  - Hoptroff M
AD  - Unilever R&D Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, 
      Shanghai, 200335, China.
FAU - Jones, Andrew
AU  - Jones A
AD  - Unilever R&D Port Sunlight, Wirral, Bebington, Merseyside, CH63 3JW, UK.
FAU - Collins, Luisa Z
AU  - Collins LZ
AD  - Unilever R&D Port Sunlight, Wirral, Bebington, Merseyside, CH63 3JW, UK.
FAU - Janssen, Hans-Gerd
AU  - Janssen HG
AD  - Analytical-Chemistry Group, Van't Hoff Institute for Molecular Sciences, 
      University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.
AD  - Unilever Research and Development Vlaardingen, P.O. Box 114, 3130 AC, 
      Vlaardingen, The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20160129
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 7QWM220FJH (Squalene)
SB  - IM
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Hair/*chemistry
MH  - Humans
MH  - Limit of Detection
MH  - Spectrum Analysis, Raman/*methods
MH  - Squalene/*analysis
OTO - NOTNLM
OT  - Cuticle to medulla
OT  - GC-MS
OT  - Human hair
OT  - Raman imaging
OT  - Root to tip
OT  - Squalene
EDAT- 2016/01/31 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/01/31 06:00
PHST- 2015/11/02 00:00 [received]
PHST- 2016/01/13 00:00 [accepted]
PHST- 2016/01/06 00:00 [revised]
PHST- 2016/01/31 06:00 [entrez]
PHST- 2016/01/31 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1007/s00216-016-9335-0 [pii]
AID - 10.1007/s00216-016-9335-0 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2016 Mar;408(9):2357-62. doi: 10.1007/s00216-016-9335-0. Epub 
      2016 Jan 29.