PMID- 26825574
OWN - NLM
STAT- MEDLINE
DCOM- 20170221
LR  - 20181113
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 36
IP  - 8
DP  - 2016 Nov
TI  - Low-Dose Homocystine Enhances Proliferation and Migration of Bv2 Microglia Cells.
PG  - 1279-1289
AB  - Homocysteine (Hcy) is a non-essential amino acid that is derived from the 
      breakdown of dietary methionine. Hyperhomocysteinemia (HHcy) is an independent 
      risk factor for a variety of chronic diseases, especially neurodegenerative 
      conditions. To better understand the role of HHcy in the pathogenesis of 
      neurodegenerative disorders, we investigated the effect of Hcy on the 
      proliferation and activation of microglia Bv2 cells. Cells were treated with six 
      different Hcy concentrations: 0, 50, 100, 300, 500, and 1000 microM for different 
      time periods (8, 12, 16, 24, and 48 h). The morphology of Bv2 cells was observed, 
      and cell activity and proliferation were detected. Cell migration and secretion 
      of pro-inflammatory cytokines were detected by the scratch wound assay, the 
      transwell assay, and ELISA, respectively. The effect of Hcy on Bv2 proliferation 
      occurred earlier (<24 h, especially 16 h) after treatment with concentrations 
      between 100 and 300 muM, and there was no cytotoxicity to Bv2 cells. Meanwhile, 
      functional assays suggested that Hcy not only promoted Bv2 secretion of the 
      pro-inflammatory cytokines IL-1beta, TNF-alpha, and IL-6, but also enhanced Bv2 
      migration and invasion, with 100 muM being the most effective concentration. In 
      summary, Bv2 proliferation and activation can be promoted by short-term treatment 
      with low-dose Hcy.
FAU - Wan, Lishu
AU  - Wan L
AD  - Department of Neurology, The First Hospital of Dandong, Baoshan Street No. 76, 
      Yuanbao District, Dandong city, 118000, Liaoning Province, China. 
      wanlishu_dandong@163.com.
FAU - Sun, Yingjie
AU  - Sun Y
AD  - Department of Medicine, The Central Hospital of Dandong, Dandong, China.
FAU - Zhang, Fan
AU  - Zhang F
AD  - Clinical Medicine Laboratory, The Medical College of Eastern Liaoning University, 
      Dandong, China.
FAU - Ren, Yan
AU  - Ren Y
AD  - Department of Neurology, The First Hospital of China Medical University, 
      Shenyang, China.
LA  - eng
PT  - Journal Article
DEP - 20160129
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Cytokines)
RN  - 462-10-2 (Homocystine)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cytokines/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Homocystine/*pharmacology
MH  - Microglia/*drug effects/physiology
MH  - Neurodegenerative Diseases/etiology/metabolism
OTO - NOTNLM
OT  - Activation
OT  - Bv2 cells
OT  - Homocysteine
OT  - Proliferation
EDAT- 2016/01/31 06:00
MHDA- 2017/02/22 06:00
CRDT- 2016/01/31 06:00
PHST- 2015/09/28 00:00 [received]
PHST- 2015/12/23 00:00 [accepted]
PHST- 2016/01/31 06:00 [pubmed]
PHST- 2017/02/22 06:00 [medline]
PHST- 2016/01/31 06:00 [entrez]
AID - 10.1007/s10571-015-0325-0 [pii]
AID - 10.1007/s10571-015-0325-0 [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2016 Nov;36(8):1279-1289. doi: 10.1007/s10571-015-0325-0. 
      Epub 2016 Jan 29.