PMID- 26826553
OWN - NLM
STAT- MEDLINE
DCOM- 20171201
LR  - 20220316
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 7
IP  - 10
DP  - 2016 Mar 8
TI  - miR-144-3p, a tumor suppressive microRNA targeting ETS-1 in laryngeal squamous 
      cell carcinoma.
PG  - 11637-50
LID - 10.18632/oncotarget.7025 [doi]
AB  - Regional lymph node metastasis and distant metastasis are critical in the 
      prognosis of laryngeal squamous cell carcinoma (LSCC). This study investigated 
      the roles of miR-144-3p and E26 transformation specific-1 (ETS-1) in the invasion 
      and migration of LSCC cells. The effects of miR-144-3p and ETS-1 on FaDu and Hep2 
      cell growth, migration and invasion were determined. Suppression of ETS-1 by 
      miR-144-3p was confirmed using luciferase assays; the effects of ETS-1 silencing 
      were determined using a xenograft tumor model. The expression of ETS-1 was 
      analyzed in 71 paraffin-embedded tissue biopsies and eight fresh frozen biopsies 
      obtained from LSCC patients. miR-144-3p inhibited the growth, invasion and 
      migration of FaDu and Hep2 cells in part through suppression of 
      epithelial-mesenchymal transition as determined by increased E-cadherin and 
      alpha-catenin and reduced fibronectin and vimentin expression. Additionally, ETS-1 is 
      a molecular target of miR-144-3p, and silencing ETS-1 expression inhibited FaDu 
      and Hep2 cell invasion and migration as well as reduced Hep2 xenograft tumor 
      volume. In LSCC, the expression of ETS-1 is upregulated with disease progression, 
      and higher ETS-1 expression, which was negatively associated with miR-144-3p 
      levels, adversely corresponded with prognoses. Thus, upregulated ETS-1 levels may 
      promote LSCC metastasis, resulting in poor patient prognosis.
FAU - Zhang, Si-Yi
AU  - Zhang SY
AD  - Department of Otorhinolaryngology, Guangdong General Hospital and Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong Province, China.
FAU - Lu, Zhong-Ming
AU  - Lu ZM
AD  - Department of Otorhinolaryngology, Guangdong General Hospital and Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong Province, China.
FAU - Lin, Ye-Feng
AU  - Lin YF
AD  - Department of Otorhinolaryngology, Guangdong General Hospital and Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong Province, China.
FAU - Chen, Liang-Si
AU  - Chen LS
AD  - Department of Otorhinolaryngology, Guangdong General Hospital and Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong Province, China.
FAU - Luo, Xiao-Ning
AU  - Luo XN
AD  - Department of Otorhinolaryngology, Guangdong General Hospital and Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong Province, China.
FAU - Song, Xin-Han
AU  - Song XH
AD  - Department of Otorhinolaryngology, Guangdong General Hospital and Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong Province, China.
FAU - Chen, Shao-Hua
AU  - Chen SH
AD  - Department of Otorhinolaryngology, Guangdong General Hospital and Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong Province, China.
FAU - Wu, Yi-Long
AU  - Wu YL
AD  - Guangdong Lung Cancer Institute, Guangdong General Hospital and Guangdong Academy 
      of Medical Sciences, Guangzhou, Guangdong Province, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (ETS1 protein, human)
RN  - 0 (MIRN144 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Protein c-ets-1)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Disease Progression
MH  - Epithelial-Mesenchymal Transition
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*genetics
MH  - Prognosis
MH  - Proto-Oncogene Protein c-ets-1/*genetics
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Transfection
PMC - PMC4905499
OTO - NOTNLM
OT  - biomarker
OT  - laryngeal squamous cell carcinoma
OT  - miR-144-3p
OT  - migration
OT  - prognosis
COIS- CONFLICTS OF INTEREST No potential conflicts of interest were disclosed.
EDAT- 2016/01/31 06:00
MHDA- 2017/12/02 06:00
PMCR- 2016/03/08
CRDT- 2016/01/31 06:00
PHST- 2015/06/12 00:00 [received]
PHST- 2015/11/28 00:00 [accepted]
PHST- 2016/01/31 06:00 [entrez]
PHST- 2016/01/31 06:00 [pubmed]
PHST- 2017/12/02 06:00 [medline]
PHST- 2016/03/08 00:00 [pmc-release]
AID - 7025 [pii]
AID - 10.18632/oncotarget.7025 [doi]
PST - ppublish
SO  - Oncotarget. 2016 Mar 8;7(10):11637-50. doi: 10.18632/oncotarget.7025.