PMID- 26826824
OWN - NLM
STAT- MEDLINE
DCOM- 20160428
LR  - 20160201
IS  - 1011-601X (Print)
IS  - 1011-601X (Linking)
VI  - 29
IP  - 1
DP  - 2016 Jan
TI  - Effect of lipid extracts of Nigella sativa L. seeds on the liver ATP reduction 
      and alpha-glucosidase inhibition.
PG  - 111-7
AB  - Various extracts from the seeds of Nigella sativa have been used in traditional 
      folk medicine to treat inflammation, liver disorders and arthritis. These seeds 
      have been experimentally shown to possess antioxidant and hepatoprotective 
      properties. Beside the hypoglycaemic and hypolipidemic effects, this study was 
      carried out to evaluate, in vitro, toxicological effect of lipid extracts from 
      the Nigella sativa seeds. The tested fractions were: (i) defatted methanolic 
      extract, (ii) total lipid extract obtained by hexane extraction from methanolic 
      extract and (iii) neutral and polar lipid fractions. The fractions were assessed, 
      in vitro, for their inhibitory activity potential on the enzyme alpha-glucosidase 
      as suppressing the enzyme activity is one among the therapeutic approaches to 
      attenuate postprandial hyperglycemia. High inhibition of alpha-glucosidase by the 
      two polar lipid fractions (F6 and F7) was reflected by their IC50 (0.51+/-0.04mg/ml 
      and 0.55+/-0.09mg/ml, respectively), compared to acarbose (0.53+/-0.06mg/ml) and 
      thymoquinone (0.65+/-0.05mg/ml). The hypoglycaemic effect of the polar lipid 
      fraction of Nigella sativa could be explained by the inhibition of 
      alpha-glucosidase, which is one of early steps of carbohydrate metabolism. 
      Toxicological evaluation was investigated on precision-cut rat liver slices 
      (PCLS). On PCLS, lipid extracts reduced ATP levels by 27 to 35%. Results indicate 
      suggest that Nigella sativa extracts don't show a hepatoprotective effect against 
      acetaminophen, but don't exhibit a major hepatotoxicity when tested alone.
FAU - Sobhi, Widad
AU  - Sobhi W
AD  - Laboratory of Applied Biochemistry, Faculty of Life and Nature Sciences, 
      University of Ferhat Abbas Setif 1, Algeria.
FAU - Stevigny, Caroline
AU  - Stevigny C
AD  - Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Institute of 
      Pharmacy, UniversiteLibre de Bruxelles, Belgium.
FAU - Duez, Pierre
AU  - Duez P
AD  - Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Institute of 
      Pharmacy, UniversiteLibre de Bruxelles, Belgium.
FAU - Calderon, Bedro Buc
AU  - Calderon BB
AD  - Metabolism and Nutrition group (MNut), Louain Drug Research Institute, Catholic 
      university of Louvain , 1200 Belgium.
FAU - Atmani, Djebbar
AU  - Atmani D
AD  - Department of Physical and Chemical Biology, Faculty of Life and Nature Sciences, 
      University of Bejaia; Algeria.
FAU - Benboubetra, Mustapha
AU  - Benboubetra M
AD  - Laboratory of Applied Biochemistry, Faculty of Life and Nature Sciences, 
      University of Ferhat Abbas Setif 1, Algeria.
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Pharm Sci
JT  - Pakistan journal of pharmaceutical sciences
JID - 9426356
RN  - 0 (Glycoside Hydrolase Inhibitors)
RN  - 0 (Plant Extracts)
RN  - 5W494URQ81 (Streptozocin)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
SB  - IM
MH  - Adenosine Triphosphate/*metabolism
MH  - Animals
MH  - Diabetes Mellitus, Experimental/blood/drug therapy
MH  - Female
MH  - Glycoside Hydrolase Inhibitors/*pharmacology
MH  - Liver/*drug effects/metabolism
MH  - *Nigella sativa
MH  - Plant Extracts/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Seeds
MH  - Streptozocin
MH  - alpha-Glucosidases/metabolism
EDAT- 2016/02/02 06:00
MHDA- 2016/04/29 06:00
CRDT- 2016/02/01 06:00
PHST- 2016/02/01 06:00 [entrez]
PHST- 2016/02/02 06:00 [pubmed]
PHST- 2016/04/29 06:00 [medline]
PST - ppublish
SO  - Pak J Pharm Sci. 2016 Jan;29(1):111-7.