PMID- 26828137
OWN - NLM
STAT- MEDLINE
DCOM- 20160824
LR  - 20181202
IS  - 1752-7163 (Electronic)
IS  - 1752-7155 (Linking)
VI  - 10
IP  - 1
DP  - 2016 Feb 1
TI  - Oxidative stress markers are elevated in exhaled breath condensate of workers 
      exposed to nanoparticles during iron oxide pigment production.
PG  - 016004
LID - 10.1088/1752-7155/10/1/016004 [doi]
AB  - Markers of oxidative stress and inflammation were analysed in the exhaled breath 
      condensate (EBC) and urine samples of 14 workers (mean age 43  +/-  7 years) 
      exposed to iron oxide aerosol for an average of 10  +/-  4 years and 14 controls 
      (mean age 39  +/-  4 years) by liquid chromatography-electrospray ionization-mass 
      spectrometry/mass spectrometry (LC-ESI-MS/MS) after solid-phase extraction. 
      Aerosol exposure in the workplace was measured by particle size spectrometers, a 
      scanning mobility particle sizer (SMPS) and an aerodynamic particle sizer (APS), 
      and by aerosol concentration monitors, P-TRAK and DustTRAK DRX. Total aerosol 
      concentrations in workplace locations varied greatly in both time and space. The 
      median mass concentration was 0.083 mg m(-3) (IQR 0.063-0.133 mg m(-3)) and the 
      median particle concentration was 66 800 particles cm(-3) (IQR 16,900-86,900 
      particles cm(-3)). In addition, more than 80% of particles were smaller than 
      100 nm in diameter. Markers of oxidative stress, malondialdehyde (MDA), 
      4-hydroxy-trans-hexenale (HHE), 4-hydroxy-trans-nonenale (HNE), 
      8-isoProstaglandin F2alpha (8-isoprostane) and aldehydes C6-C12, in addition to 
      markers of nucleic acid oxidation, including 8-hydroxy-2-deoxyguanosine (8-OHdG), 
      8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), and of proteins, 
      such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine 
      (3-NOTyr) were analysed in EBC and urine by LC-ESI-MS/MS. Almost all markers of 
      lipid, nucleic acid and protein oxidation were elevated in the EBC of workers 
      comparing with control subjects. Elevated markers were MDA, HNE, HHE, C6-C10, 
      8-isoprostane, 8-OHdG, 8-OHG, 5-OHMeU, 3-ClTyr, 3-NOTyr, o-Tyr (all p  <  0.001), 
      and C11 (p  <  0.05). Only aldehyde C12 and the pH of samples did not differ 
      between groups. Markers in urine were not elevated. These findings suggest the 
      adverse effects of nano iron oxide aerosol exposure and support the utility of 
      oxidative stress biomarkers in EBC. The analysis of urine oxidative stress 
      biomarkers does not support the presence of systemic oxidative stress in iron 
      oxide pigment production workers.
FAU - Pelclova, Daniela
AU  - Pelclova D
AD  - Charles University in Prague and General University Hospital in Prague, First 
      Faculty of Medicine, Department of Occupational Medicine, Na Bojisti 1, 128 00 
      Prague 2, Czech Republic.
FAU - Zdimal, Vladimir
AU  - Zdimal V
FAU - Kacer, Petr
AU  - Kacer P
FAU - Fenclova, Zdenka
AU  - Fenclova Z
FAU - Vlckova, Stepanka
AU  - Vlckova S
FAU - Syslova, Kamila
AU  - Syslova K
FAU - Navratil, Tomas
AU  - Navratil T
FAU - Schwarz, Jaroslav
AU  - Schwarz J
FAU - Zikova, Nadezda
AU  - Zikova N
FAU - Barosova, Hana
AU  - Barosova H
FAU - Turci, Francesco
AU  - Turci F
FAU - Komarc, Martin
AU  - Komarc M
FAU - Pelcl, Tomas
AU  - Pelcl T
FAU - Belacek, Jaroslav
AU  - Belacek J
FAU - Kukutschova, Jana
AU  - Kukutschova J
FAU - Zakharov, Sergey
AU  - Zakharov S
LA  - eng
PT  - Journal Article
DEP - 20160201
PL  - England
TA  - J Breath Res
JT  - Journal of breath research
JID - 101463871
RN  - 0 (Aldehydes)
RN  - 0 (Biomarkers)
RN  - 0 (Ferric Compounds)
RN  - 12133JR80S (Guanosine)
RN  - 1K09F3G675 (ferric oxide)
RN  - 27415-26-5 (8-epi-prostaglandin F2alpha)
RN  - 3604-79-3 (3-nitrotyrosine)
RN  - 3868-31-3 (8-hydroxyguanosine)
RN  - 42HK56048U (Tyrosine)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - B7IN85G1HY (Dinoprost)
RN  - SY44C9MINA (3-chlorotyrosine)
SB  - IM
MH  - Adult
MH  - Aldehydes/analysis
MH  - Biomarkers/analysis
MH  - Breath Tests
MH  - Dinoprost/analogs & derivatives/analysis
MH  - Ferric Compounds/*chemical synthesis
MH  - Guanosine/analogs & derivatives/analysis
MH  - Humans
MH  - Male
MH  - Malondialdehyde/analysis
MH  - Middle Aged
MH  - Nanoparticles/*toxicity
MH  - Oxidative Stress/drug effects/*physiology
MH  - Tandem Mass Spectrometry
MH  - Tyrosine/analogs & derivatives/analysis
EDAT- 2016/02/02 06:00
MHDA- 2016/08/25 06:00
CRDT- 2016/02/02 06:00
PHST- 2016/02/02 06:00 [entrez]
PHST- 2016/02/02 06:00 [pubmed]
PHST- 2016/08/25 06:00 [medline]
AID - 10.1088/1752-7155/10/1/016004 [doi]
PST - epublish
SO  - J Breath Res. 2016 Feb 1;10(1):016004. doi: 10.1088/1752-7155/10/1/016004.