PMID- 26828261
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 50
DP  - 2016 Mar
TI  - Molecular cloning, characterization and mRNA expression of six peroxiredoxins 
      from Black carp Mylopharyngodon piceus in response to lipopolysaccharide 
      challenge or dietary carbohydrate.
PG  - 210-22
LID - S1050-4648(16)30033-X [pii]
LID - 10.1016/j.fsi.2016.01.033 [doi]
AB  - Peroxiredoxin (Prx) belongs to a cellular antioxidant protein family that plays 
      important roles in innate immune function and anti-oxidative capability. In the 
      present study, six Prxs were cloned from Black carp Mylopharyngodon piceus 
      (MpPrx) by homology cloning and rapid amplification of cDNA ends (RACE) 
      techniques. There were 199, 197, 250, 260, 189 and 222 amino acids in six MpPrxs, 
      respectively. BLAST analysis reveals that MpPrxs shares high identities and 
      similar characteristics with other known Prxs from animals. The phylogenetic 
      analysis evidenced three major subclasses corresponding to one-Cys-Prx (MpPrx6), 
      typical two-Cys-Prx (MpPrx1-4) and atypical 2-Cys-Prx (MpPrx5) that reflected the 
      present hierarchy of vertebrates and invertebrates. Although six MpPrxs are 
      constitutively expressed in all tissues, relatively higher-level mRNA expression 
      levels of six MpPrxs can be detected in liver, eyes, heart and adipose tissues by 
      real-time PCR assays. The transcriptional patterns of six MpPrxs mRNA in liver 
      were detected by real-time PCR in Black carp after lipopolysaccharide (LPS) 
      challenge and treated with graded levels of dietary carbohydrate (CHO) (106.5, 
      194.3, 288.4 and 379.1 g kg(-1)), respectively. These results showed that 
      stimulation with LPS could induce up-expression of six MpPrxs mRNA, and the 
      variations of MpPrx4 were more sensitive than these of other MpPrxs in the liver 
      of Black carp. Compared with those in group with 106.5 g kg(-1) dietary CHO, the 
      expression levels of MpPrx2, MpPrx3 and MpPrx6 were significantly down-regulated 
      while MpPrx5 were significantly induced in liver of Black carp fed with adequate 
      dietary CHO (194.3 g kg(-1)). In addition, significant up-regulations of MpPrx2, 
      MpPrx3 and MpPrx6 were observed in Black carp fed with excessive dietary CHO 
      (379.1 g kg(-1)). And MpPrx4 could be constantly induced with increasing dietary 
      CHO contents in this study. These results indicated that MpPrxs were constitutive 
      and inducible proteins and might play important roles in innate immune function 
      after LPS challenge and regulating redox homeostasis in the metabolism of dietary 
      CHO.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Wu, Chenglong
AU  - Wu C
AD  - School of Life Science, Huzhou University, 759 Erhuan Road (E), Huzhou, 313000, 
      PR China. Electronic address: wuchenglong007@163.com.
FAU - Gao, Jun'e
AU  - Gao J
AD  - School of Life Science, Huzhou University, 759 Erhuan Road (E), Huzhou, 313000, 
      PR China.
FAU - Cao, Fang
AU  - Cao F
AD  - School of Life Science, Huzhou University, 759 Erhuan Road (E), Huzhou, 313000, 
      PR China.
FAU - Lu, Zhibin
AU  - Lu Z
AD  - School of Life Science, Huzhou University, 759 Erhuan Road (E), Huzhou, 313000, 
      PR China.
FAU - Chen, Lian
AU  - Chen L
AD  - School of Life Science, Huzhou University, 759 Erhuan Road (E), Huzhou, 313000, 
      PR China.
FAU - Ye, Jinyun
AU  - Ye J
AD  - School of Life Science, Huzhou University, 759 Erhuan Road (E), Huzhou, 313000, 
      PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160130
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (DNA, Complementary)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Fish Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - EC 1.11.1.15 (Peroxiredoxins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carps/*genetics/immunology/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/metabolism
MH  - Dietary Carbohydrates/*pharmacology
MH  - Fish Proteins/chemistry/*genetics/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Peroxiredoxins/chemistry/*genetics/metabolism
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Sequence Alignment/veterinary
OTO - NOTNLM
OT  - Dietary carbohydrate
OT  - Lipopolysaccharide challenge
OT  - Mylopharyngodon piceus
OT  - Peroxiredoxin
OT  - mRNA expression
EDAT- 2016/02/02 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/02 06:00
PHST- 2015/10/04 00:00 [received]
PHST- 2016/01/07 00:00 [revised]
PHST- 2016/01/27 00:00 [accepted]
PHST- 2016/02/02 06:00 [entrez]
PHST- 2016/02/02 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S1050-4648(16)30033-X [pii]
AID - 10.1016/j.fsi.2016.01.033 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Mar;50:210-22. doi: 10.1016/j.fsi.2016.01.033. Epub 
      2016 Jan 30.