PMID- 26829724
OWN - NLM
STAT- MEDLINE
DCOM- 20160421
LR  - 20191210
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 33
IP  - 1
DP  - 2016 Feb
TI  - [Application of eight-probe fluorescence in situ hybridization and R-banding 
      karyotype analysis for the diagnosis of acute lymphoblastic leukemia].
PG  - 9-12
LID - 10.3760/cma.j.issn.1003-9406.2016.01.003 [doi]
AB  - OBJECTIVE: To assess the value of eight-probe fluorescence in situ hybridization 
      (FISH) and R-banding karyotype analysis for the diagnosis of acute lymphoblastic 
      leukemia (ALL). METHODS: With the eight-probe FISH (using probes for MYC, P16, 
      E2A, CHIC2/D10Z1/D17Z1, TEL/AMLl, MLL, BCR/ABL1, and IGH) and R-banding karyotype 
      analysis, 237 cases of ALL were analyzed. RESULTS: Cytogenetic changes were 
      detected in 135 (56.96%) of all cases, which have involved MYC, P16, E2A, 
      CHIC2/D10Z1/D17Z1, TEL/AMLl, MLL, BCR/ABL1, and IGH polyploidies. R-banding 
      karyotype analysis has only detected abnormalities in 48 of such cases, in 
      addition with 14 abnormalities missed by the FISH probes, which have given a 
      total positive rate of 26.16%. The detection rate of the two methods has differed 
      significantly(P<0.05). CONCLUSION: Compared with the R-banding karyotype 
      analysis, the eight-probe FISH is more accurate and efficient. Diagnosis of 
      cytogenetic abnormalities for children with ALL using the combined method can 
      provide a basis for evaluation of prognosis as well as personalized therapy.
FAU - Zhao, Ding
AU  - Zhao D
AD  - Genetics Laboratory, Children's Hospital of Zhengzhou, Zhengzhou, Henan 450053, 
      China. 1019231544@qq.com.
FAU - Liu, Shuai
AU  - Liu S
FAU - Guo, Zhenxin
AU  - Guo Z
FAU - Li, Rui
AU  - Li R
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosome Banding/*methods
MH  - Genetic Testing/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*diagnosis/*genetics
EDAT- 2016/02/02 06:00
MHDA- 2016/04/22 06:00
CRDT- 2016/02/02 06:00
PHST- 2016/02/02 06:00 [entrez]
PHST- 2016/02/02 06:00 [pubmed]
PHST- 2016/04/22 06:00 [medline]
AID - 940632003 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2016.01.003 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2016 Feb;33(1):9-12. doi: 
      10.3760/cma.j.issn.1003-9406.2016.01.003.