PMID- 26829727
OWN - NLM
STAT- MEDLINE
DCOM- 20160421
LR  - 20160202
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 33
IP  - 1
DP  - 2016 Feb
TI  - [Calibration of false positive result in detection of BCR/ABL using fluorescence 
      in situ hybridization].
PG  - 22-5
LID - 10.3760/cma.j.issn.1003-9406.2016.01.006 [doi]
AB  - OBJECTIVE: To explore the effect of false positive signals during detection of 
      BCR/ABL fusion gene by fluorescence in situ hybridization (FISH), and develop a 
      method for calibration. METHODS: Normal specimens were mixed with BCR/ABL 
      positive specimens in which presented signal pattern of 1-red-2-green-1-fusion 
      (1R2G1F) using dual color dual fusion (DCDF) probes and 1-red-1-green-1-fusion 
      (1R1G1F) using extra signal (ES) probes in different proportions. Mixed samples 
      were detected using DCDF and ES probes. Results of DCDF probes, ES probe before 
      calibration, ES probes after calibration and theoretical results were compared by 
      binomial distribution in different proportions. RESULTS: The rate of false 
      positive signals has risen with increase of negative rate. A significant 
      difference was found between theoretical proportion and results without 
      calibration in negative level, 5%, 10% and 25% positive level (P<0.05). There was 
      no significant difference between theoretical proportion and results without 
      calibration in 50% and 90% positive level (P>0.05). Also there was no significant 
      difference between theoretical proportion and calibrated results (P>0.05). 
      CONCLUSION: Calibration of FISH result can delimitate the effect of false 
      positives, and can provide more reliable results in cases with low level positive 
      rates.
FAU - Du, Qinghua
AU  - Du Q
AD  - Department of Hematology, Guangzhou First People's Hospital, Guangzhou Medical 
      University, Guangzhou, Guangdong 510180, China. qingshanli@hotmail.com.
FAU - Li, Qingshan
AU  - Li Q
FAU - Chen, Xiaoyan
AU  - Chen X
FAU - Ying, Yi
AU  - Ying Y
FAU - Wang, Shunqing
AU  - Wang S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Calibration
MH  - False Positive Reactions
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods/*standards
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/*genetics
MH  - Young Adult
EDAT- 2016/02/02 06:00
MHDA- 2016/04/22 06:00
CRDT- 2016/02/02 06:00
PHST- 2016/02/02 06:00 [entrez]
PHST- 2016/02/02 06:00 [pubmed]
PHST- 2016/04/22 06:00 [medline]
AID - 940632006 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2016.01.006 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2016 Feb;33(1):22-5. doi: 
      10.3760/cma.j.issn.1003-9406.2016.01.006.