PMID- 26830811
OWN - NLM
STAT- MEDLINE
DCOM- 20170103
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Feb 1
TI  - Characterization of Cyclooxygenase-2 and its induction pathways in response to 
      high lipid diet-induced inflammation in Larmichthys crocea.
PG  - 19921
LID - 10.1038/srep19921 [doi]
LID - 19921
AB  - The present study was conducted to investigate the effects of a high-lipid diet 
      (HLD) on cyclooxygenase (Cox)-2 expression and the signalling pathways related to 
      low-grade inflammation in the large yellow croaker (Larmichthys crocea). An 
      isolated 2508 bp cDNA clone of cox-2 contained an open reading frame spanning 
      1827 bp encoding a protein with 608 amino acid residues. The over-expression of 
      cox-2 was consistent with the activation of c-Jun N-terminal kinases (JNKs) and 
      p38 mitogen-activated protein kinase (MAPK) in HLD-fed fish. The activation of 
      the activator protein-1 (AP-1) and the nuclear transcription factor kappa-B 
      (NF-kappaB) signalling pathways in HLD-fed fish and the significant increase of cox-2 
      promoter-luciferase activity in vitro indicated that AP-1 and NF-kappaB could combine 
      cox-2 promoter to promote its transcription, respectively. Together, HLD-induced 
      inflammation up-regulates cox-2 expression via JNKs and p38 MAPK-dependent NF-kappaB 
      and AP-1 pathways. The present study provides important insight into the signal 
      transduction pathways involved in HLD-induced inflammation, which is detrimental 
      to the health and production of fish as well as to the health of fish consumers.
FAU - Wang, Tianjiao
AU  - Wang T
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Yan, Jing
AU  - Yan J
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Xu, Wei
AU  - Xu W
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Ai, Qinghui
AU  - Ai Q
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Mai, Kangsen
AU  - Mai K
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160201
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Dietary Fats)
RN  - 0 (Fish Proteins)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Cyclooxygenase 2/*biosynthesis
MH  - Dietary Fats/*adverse effects/pharmacology
MH  - Enzyme Induction/drug effects
MH  - Fish Diseases/chemically induced/*enzymology
MH  - Fish Proteins/*biosynthesis
MH  - Fishes/*metabolism
MH  - Inflammation/chemically induced/enzymology
PMC - PMC4735279
EDAT- 2016/02/03 06:00
MHDA- 2017/01/04 06:00
PMCR- 2016/02/01
CRDT- 2016/02/03 06:00
PHST- 2015/08/21 00:00 [received]
PHST- 2015/12/21 00:00 [accepted]
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2017/01/04 06:00 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - srep19921 [pii]
AID - 10.1038/srep19921 [doi]
PST - epublish
SO  - Sci Rep. 2016 Feb 1;6:19921. doi: 10.1038/srep19921.