PMID- 26831424
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20220331
IS  - 0971-5916 (Print)
IS  - 0971-5916 (Linking)
VI  - 142
IP  - 6
DP  - 2015 Dec
TI  - Culture & differentiation of mesenchymal stem cell into osteoblast on degradable 
      biomedical composite scaffold: In vitro study.
PG  - 747-58
LID - 10.4103/0971-5916.174568 [doi]
AB  - BACKGROUND & OBJECTIVES: There is a significant bone tissue loss in patients from 
      diseases and traumatic injury. The current autograft transplantation gold 
      standard treatment has drawbacks, namely donor site morbidity and limited supply. 
      The field of tissue engineering has emerged with a goal to provide alternative 
      sources for transplantations to bridge this gap between the need and lack of bone 
      graft. The aim of this study was to prepare biocomposite scaffolds based on 
      chitosan (CHT), polycaprolactone (PCL) and hydroxyapatite (HAP) by freeze drying 
      method and to assess the role of scaffolds in spatial organization, 
      proliferation, and osteogenic differentiation of human mesenchymal stem cells 
      (hMSCs) in vitro, in order to achieve bone graft substitutes with improved 
      physical-chemical and biological properties. METHODS: Pure chitosan (100CHT) and 
      composites (40CHT/HAP, 30CHT/HAP/PCL and 25CHT/HAP/PCL scaffolds containing 40, 
      30, 25 parts per hundred resin (phr) filler, respectively) in acetic acid were 
      freeze dried and the porous foams were studied for physicochemical and in vitro 
      biological properties. RESULTS: Scanning electron microscope (SEM) images of the 
      scaffolds showed porous microstructure (20-300 mum) with uniform pore distribution 
      in all compositions. Materials were tested under compressive load in wet 
      condition (using phosphate buffered saline at pH 7.4). The in vitro studies 
      showed that all the scaffold compositions supported mesenchymal stem cell 
      attachment, proliferation and differentiation as visible from SEM images, 
      [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay, 
      alkaline phosphatase (ALP) assay and quantitative reverse transcription 
      (qRT)-PCR. INTERPRETATION & CONCLUSIONS: Scaffold composition 25CHT/HAP/PCL 
      showed better biomechanical and osteoinductive properties as evident by 
      mechanical test and alkaline phosphatase activity and osteoblast specific gene 
      expression studies. This study suggests that this novel degradable 3D composite 
      may have great potential to be used as scaffold in bone tissue engineering.
FAU - Jain, Krishan G
AU  - Jain KG
FAU - Mohanty, Sujata
AU  - Mohanty S
AD  - Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India.
FAU - Ray, Alok R
AU  - Ray AR
FAU - Malhotra, Rajesh
AU  - Malhotra R
FAU - Airan, Balram
AU  - Airan B
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
RN  - 0 (Polyesters)
RN  - 24980-41-4 (polycaprolactone)
RN  - 9012-76-4 (Chitosan)
RN  - 91D9GV0Z28 (Durapatite)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Chitosan
MH  - Durapatite
MH  - Humans
MH  - In Vitro Techniques
MH  - Mesenchymal Stem Cells/*cytology/enzymology
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/*cytology
MH  - Polyesters
MH  - *Tissue Scaffolds
PMC - PMC4774072
EDAT- 2016/02/03 06:00
MHDA- 2016/11/01 06:00
PMCR- 2015/12/01
CRDT- 2016/02/03 06:00
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
AID - IndianJMedRes_2015_142_6_747_174568 [pii]
AID - IJMR-142-747 [pii]
AID - 10.4103/0971-5916.174568 [doi]
PST - ppublish
SO  - Indian J Med Res. 2015 Dec;142(6):747-58. doi: 10.4103/0971-5916.174568.