PMID- 26832678
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20201209
IS  - 1860-2002 (Electronic)
IS  - 1536-1632 (Linking)
VI  - 18
IP  - 4
DP  - 2016 Aug
TI  - Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against 
      its Ribosomal RNA.
PG  - 527-34
LID - 10.1007/s11307-015-0927-4 [doi]
AB  - PURPOSE: Previously, we demonstrated specific accumulation into bacteria of a 
      12-mer phosphorodiamidate morpholino (MORF) oligomer complementary to a ribosomal 
      RNA (rRNA) segment found in all bacteria using the universal probe called Eub338 
      (Eub). Here, two MORF oligomers Eco and Kpn with sequences specific to the rRNA 
      of Escherichia coli (Eco) and Klebsiella pneumoniae (Kpn) were investigated along 
      with Eub and control (nonEub). PROCEDURES: To determine bacterial rRNA binding, 
      oligomers were tagged with Alexa Fluor 633 (AF633) for fluorescence in situ 
      hybridization (FISH) and fluorescence microscopy, and radiolabeled with 
      technetium-99m (Tc-99m) for biodistribution and SPECT imaging in infected mice. 
      RESULTS: By both FISH and fluorescence microscopy, Eub showed a positive signal 
      in both E. coli and K. pneumoniae as expected, and Kpn showed significantly 
      higher accumulation in K. pneumoniae with near background in E. coli (p < 0.01). 
      Conversely, Eco was positive in both E. coli and K. pneumoniae, hence 
      nonspecific. As determined by biodistribution, the accumulation of [(99m)Tc]Kpn 
      was higher in the thigh infected with live K. pneumoniae than with live E. coli 
      (p = 0.05), and significantly higher than with heat-killed K. pneumoniae 
      (p = 0.02) in the target thigh. By SPECT imaging, the accumulation of 
      [(99m)Tc]Kpn was obviously higher in its specific target of K. pneumoniae 
      compared to an E. coli infected thigh. CONCLUSIONS: Kpn complementary to the rRNA 
      of K. pneumoniae, labeled with Tc-99m or AF633, demonstrated specific binding to 
      fixed and live K. pneumoniae in culture and in infected mice such that 
      Tc-99m-labeled Kpn as the MORF oligomer may be useful for K. pneumoniae infection 
      detection through imaging.
FAU - Chen, Ling
AU  - Chen L
AD  - Division of Nuclear Medicine, Department of Radiology, University of 
      Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
FAU - Cheng, Dengfeng
AU  - Cheng D
AD  - Department of Nuclear Medicine, Zhongshan Hospital, Fudan University, Shanghai, 
      200032, China.
FAU - Liu, Guozheng
AU  - Liu G
AD  - Division of Nuclear Medicine, Department of Radiology, University of 
      Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
FAU - Dou, Shuping
AU  - Dou S
AD  - Division of Nuclear Medicine, Department of Radiology, University of 
      Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
FAU - Wang, Yuzhen
AU  - Wang Y
AD  - Division of Nuclear Medicine, Department of Radiology, University of 
      Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
FAU - Liu, Xinrong
AU  - Liu X
AD  - ImmunoGen Inc, Waltham, MA, 02451, USA.
FAU - Liu, Yuxia
AU  - Liu Y
AD  - Shanghai Institute of Applied Physics, Chinese Academy of Sciences, , Shanghai, 
      201800, China.
FAU - Rusckowski, Mary
AU  - Rusckowski M
AD  - Division of Nuclear Medicine, Department of Radiology, University of 
      Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA. 
      mary.rusckowski@umassmed.edu.
LA  - eng
GR  - R21 AI070857/AI/NIAID NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Mol Imaging Biol
JT  - Molecular imaging and biology
JID - 101125610
RN  - 0 (Morpholinos)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (Radiopharmaceuticals)
RN  - 7440-26-8 (Technetium)
SB  - IM
MH  - Animals
MH  - In Situ Hybridization, Fluorescence
MH  - Klebsiella Infections/*diagnosis/diagnostic imaging/*microbiology/pathology
MH  - Klebsiella pneumoniae/*isolation & purification
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Morpholinos/pharmacology
MH  - Oligonucleotides, Antisense/*pharmacology
MH  - RNA, Ribosomal/*metabolism
MH  - Radiopharmaceuticals/metabolism
MH  - Technetium/metabolism
MH  - Tissue Distribution/drug effects
MH  - Tomography, Emission-Computed, Single-Photon
MH  - Tomography, X-Ray Computed
OTO - NOTNLM
OT  - Antisense MORF
OT  - Bacterial infection imaging
OT  - Bacterial ribosomal RNA
EDAT- 2016/02/03 06:00
MHDA- 2017/09/28 06:00
CRDT- 2016/02/03 06:00
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
AID - 10.1007/s11307-015-0927-4 [pii]
AID - 10.1007/s11307-015-0927-4 [doi]
PST - ppublish
SO  - Mol Imaging Biol. 2016 Aug;18(4):527-34. doi: 10.1007/s11307-015-0927-4.