PMID- 26832903
OWN - NLM
STAT- MEDLINE
DCOM- 20160712
LR  - 20181202
IS  - 1001-9391 (Print)
IS  - 1001-9391 (Linking)
VI  - 33
IP  - 10
DP  - 2015 Oct
TI  - [Role of tumor necrosis factor-alpha in spinal cord injury of rabbits with 
      decompression sickness].
PG  - 771-3
AB  - OBJECTIVE: To observe the pathological changes in rabbits with spinal cord injury 
      induced by decompression sickness (DCS), and to investigate the role of tumor 
      necrosis factor-alpha (TNF-alpha) in spinal cord injury induced by DCS. METHODS: 
      Rabbits were randomly divided into normal control group, DCS group, and safe 
      decompression group. The rabbit model of DCS was established. Light microscopy, 
      real-time PCR, and immunohistochemical method were used to observe the 
      pathomorphological changes in the thoracolumbar spinal cord and the mRNA and 
      protein expression of TNF-alpha, respectively. The terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) was used to observe the 
      apoptosis in the spinal cord. RESULTS: In the DCS group, cavities formed in the 
      white matter of spinal cord and gliosis occurred around necrotic areas. Moreover, 
      the mRNA and protein expression of TNF-alpha was significantly higher in the DCS 
      group than in the normal control group and the safe decompression group (P<0.01). 
      The results of TUNEL showed that the number of positive apoptotic cells was 
      significantly larger in the DCS group than in the normal control group and the 
      safe decompression group (P<0.05). CONCLUSION: Apoptosis plays an important role 
      in spinal cord injury induced by DCS. In the early stage of DCS, the massive 
      release of TNF-alpha initiates apoptosis and contributes to the pathological changes 
      in spinal cord injury induced by DCS.
FAU - Wang, Cuicui
AU  - Wang C
AD  - Department of Pathology, General Hospital of Jinan Military Command, Jinan 
      250031, China.
FAU - Liu, Xiaohong
AU  - Liu X
FAU - Qi, Ruixia
AU  - Qi R
FAU - Cao, Yongcheng
AU  - Cao Y
FAU - Mao, Ruiqi
AU  - Mao R
FAU - Bi, Liquan
AU  - Bi L
FAU - Geng, Ming
AU  - Geng M
AD  - E-mail: gm2227@sina.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
JT  - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng 
      zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
JID - 8410840
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Decompression Sickness/*metabolism/pathology
MH  - Disease Models, Animal
MH  - In Situ Nick-End Labeling
MH  - RNA, Messenger
MH  - Rabbits
MH  - Spinal Cord/pathology
MH  - Spinal Cord Injuries/*metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2016/02/03 06:00
MHDA- 2016/07/13 06:00
CRDT- 2016/02/03 06:00
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2016/07/13 06:00 [medline]
PST - ppublish
SO  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2015 Oct;33(10):771-3.