PMID- 26833042
OWN - NLM
STAT- MEDLINE
DCOM- 20170410
LR  - 20211204
IS  - 1615-2573 (Electronic)
IS  - 0910-8327 (Linking)
VI  - 31
IP  - 10
DP  - 2016 Oct
TI  - Repetitive restriction of muscle blood flow enhances mTOR signaling pathways in a 
      rat model.
PG  - 1685-95
LID - 10.1007/s00380-016-0801-6 [doi]
AB  - Skeletal muscle is a plastic organ that adapts its mass to various stresses by 
      affecting pathways that regulate protein synthesis and degradation. This study 
      investigated the effects of repetitive restriction of muscle blood flow (RRMBF) 
      on microvascular oxygen pressure (PmvO2), mammalian target of rapamycin (mTOR) 
      signaling pathways, and transcripts associated with proteolysis in rat skeletal 
      muscle. Eleven-week-old male Wistar rats under anesthesia underwent six RRMBF 
      consisting of an external compressive force of 100 mmHg for 5 min applied to the 
      proximal portion of the right thigh, each followed by 3 min rest. During RRMBF, 
      PmvO2 was measured by phosphorescence quenching techniques. The total RNA and 
      protein of the tibialis anterior muscle were obtained from control rats, and rats 
      treated with RRMBF 0-6 h after the stimuli. The protein expression and 
      phosphorylation of various signaling proteins were determined by western 
      blotting. The mRNA expression level was measured by real-time RT-PCR analysis. 
      The total muscle weight increased in rats 0 h after RRMBF, but not in rats 1-6 h. 
      During RRMBF, PmvO2 significantly decreased (36.1 +/- 5.7 to 5.9 +/- 1.7 torr), and 
      recovered at rest period. RRMBF significantly increased phosphorylation of p70 
      S6-kinase (p70S6k), a downstream target of mTOR, and ribosomal protein S6 1 h 
      after the stimuli. The protein level of REDD1 and phosphorylation of AMPK and 
      MAPKs did not change. The mRNA expression levels of FOXO3a, MuRF-1, and myostatin 
      were not significantly altered. These results suggested that RRMBF significantly 
      decreased PmvO2, and enhanced mTOR signaling pathways in skeletal muscle using a 
      rat model, which may play a role in diminishing muscle atrophy under various 
      conditions in human studies.
FAU - Nakajima, Toshiaki
AU  - Nakajima T
AUID- ORCID: 0000-0001-9545-7529
AD  - Department of Cardiovascular Medicine, Dokkyo Medical University and Heart 
      Center, Dokkyo Medical University Hospital, 880 Kitakobayashi, Mibu, Tochigi, 
      321-0293, Japan. nakat@dokkyomed.ac.jp.
FAU - Yasuda, Tomohiro
AU  - Yasuda T
AD  - Seirei Christopher University, Shizuoka, Japan.
FAU - Koide, Seiichiro
AU  - Koide S
AD  - Department of Engineering Science, Bioscience and Technology Program, University 
      of Electro-Communications, Tokyo, Japan.
FAU - Yamasoba, Tatsuya
AU  - Yamasoba T
AD  - Department of Otolaryngology, University of Tokyo, Tokyo, Japan.
FAU - Obi, Syotaro
AU  - Obi S
AD  - Department of Cardiovascular Medicine and Research Support Center, Dokkyo Medical 
      Univerasity, Tochigi, Japan.
FAU - Toyoda, Shigeru
AU  - Toyoda S
AD  - Department of Cardiovascular Medicine, Dokkyo Medical University and Heart 
      Center, Dokkyo Medical University Hospital, 880 Kitakobayashi, Mibu, Tochigi, 
      321-0293, Japan.
FAU - Sato, Yoshiaki
AU  - Sato Y
AD  - Department of Basic Sciences in Medicine, Kaatsu International University, 
      Battaramulla, Sri Lanka.
FAU - Inoue, Teruo
AU  - Inoue T
AD  - Department of Cardiovascular Medicine, Dokkyo Medical University and Heart 
      Center, Dokkyo Medical University Hospital, 880 Kitakobayashi, Mibu, Tochigi, 
      321-0293, Japan.
FAU - Kano, Yutaka
AU  - Kano Y
AD  - Department of Engineering Science, Bioscience and Technology Program, University 
      of Electro-Communications, Tokyo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160201
PL  - Japan
TA  - Heart Vessels
JT  - Heart and vessels
JID - 8511258
RN  - 0 (RNA, Messenger)
RN  - 0 (Ribosomal Protein S6)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Hypoxia/*metabolism
MH  - Male
MH  - Muscle, Skeletal/*metabolism
MH  - Muscular Atrophy
MH  - Phosphorylation
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Ribosomal Protein S6/*metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/*metabolism
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Blood flow restriction
OT  - Hypoxia
OT  - Mammalian target of rapamycin (mTOR)
OT  - Microvascular pO2
OT  - Muscle atrophy
OT  - Myostatin
OT  - Rat skeletal muscle
OT  - p70 S6-kinase
EDAT- 2016/02/03 06:00
MHDA- 2017/04/11 06:00
CRDT- 2016/02/03 06:00
PHST- 2015/10/20 00:00 [received]
PHST- 2016/01/15 00:00 [accepted]
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2017/04/11 06:00 [medline]
AID - 10.1007/s00380-016-0801-6 [pii]
AID - 10.1007/s00380-016-0801-6 [doi]
PST - ppublish
SO  - Heart Vessels. 2016 Oct;31(10):1685-95. doi: 10.1007/s00380-016-0801-6. Epub 2016 
      Feb 1.