PMID- 26833898
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20240325
IS  - 1521-3773 (Electronic)
IS  - 1433-7851 (Print)
IS  - 1433-7851 (Linking)
VI  - 55
IP  - 10
DP  - 2016 Mar 1
TI  - Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes.
PG  - 3463-7
LID - 10.1002/anie.201509038 [doi]
AB  - Chemical probes capable of reacting with KS (ketosynthase)-bound biosynthetic 
      intermediates were utilized for the investigation of the model type I iterative 
      polyketide synthase 6-methylsalicylic acid synthase (6-MSAS) in vivo and in 
      vitro. From the fermentation of fungal and bacterial 6-MSAS hosts in the presence 
      of chain termination probes, a full range of biosynthetic intermediates was 
      isolated and characterized for the first time. Meanwhile, in vitro studies of 
      recombinant 6-MSA synthases with both nonhydrolyzable and hydrolyzable substrate 
      mimics have provided additional insights into substrate recognition, providing 
      the basis for further exploration of the enzyme catalytic activities.
CI  - (c) 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
FAU - Parascandolo, James S
AU  - Parascandolo JS
AD  - Department of Chemistry, University of Warwick, Library Road, Coventry, CV4 7AL, 
      UK.
FAU - Havemann, Judith
AU  - Havemann J
AD  - Department of Chemistry, University of Warwick, Library Road, Coventry, CV4 7AL, 
      UK.
FAU - Potter, Helen K
AU  - Potter HK
AD  - Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 
      1EW, UK.
FAU - Huang, Fanglu
AU  - Huang F
AD  - Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 
      1EW, UK.
AD  - Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, 
      Cambridge, CB2 1GA, UK.
FAU - Riva, Elena
AU  - Riva E
AD  - Department of Chemistry, University of Warwick, Library Road, Coventry, CV4 7AL, 
      UK.
FAU - Connolly, Jack
AU  - Connolly J
AD  - Department of Chemistry, University of Warwick, Library Road, Coventry, CV4 7AL, 
      UK.
AD  - School of Biosciences, The University of Birmingham, Birmingham, B15 2TT, UK.
FAU - Wilkening, Ina
AU  - Wilkening I
AD  - Department of Chemistry, University of Warwick, Library Road, Coventry, CV4 7AL, 
      UK.
FAU - Song, Lijiang
AU  - Song L
AD  - Department of Chemistry, University of Warwick, Library Road, Coventry, CV4 7AL, 
      UK.
FAU - Leadlay, Peter F
AU  - Leadlay PF
AD  - Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, 
      Cambridge, CB2 1GA, UK.
FAU - Tosin, Manuela
AU  - Tosin M
AD  - Department of Chemistry, University of Warwick, Library Road, Coventry, CV4 7AL, 
      UK. m.tosin@warwick.ac.uk.
LA  - eng
GR  - BB/J007250/1/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160202
PL  - Germany
TA  - Angew Chem Int Ed Engl
JT  - Angewandte Chemie (International ed. in English)
JID - 0370543
RN  - 0 (Molecular Probes)
RN  - 0 (Salicylates)
RN  - L5352FE23Y (6-methylsalicylic acid)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - *Molecular Probes
MH  - Salicylates/*metabolism
PMC - PMC4797705
OTO - NOTNLM
OT  - chemical probes
OT  - dehydratase
OT  - iterative polyketide catalysis
EDAT- 2016/02/03 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/03/18
CRDT- 2016/02/03 06:00
PHST- 2015/09/26 00:00 [received]
PHST- 2015/11/19 00:00 [revised]
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/03/18 00:00 [pmc-release]
AID - ANIE201509038 [pii]
AID - 10.1002/anie.201509038 [doi]
PST - ppublish
SO  - Angew Chem Int Ed Engl. 2016 Mar 1;55(10):3463-7. doi: 10.1002/anie.201509038. 
      Epub 2016 Feb 2.