PMID- 26834123
OWN - NLM
STAT- MEDLINE
DCOM- 20170405
LR  - 20181113
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 145
IP  - 2
DP  - 2016 Feb
TI  - Maternal T-Cell Engraftment Interferes With Human Leukocyte Antigen Typing in 
      Severe Combined Immunodeficiency.
PG  - 251-7
LID - 10.1093/ajcp/aqv079 [doi]
AB  - OBJECTIVES: To report the laboratory investigation of a case of severe combined 
      immunodeficiency (SCID) with maternal T-cell engraftment, focusing on the 
      interference of human leukocyte antigen (HLA) typing by blood chimerism. METHODS: 
      HLA typing was performed with three different methods, including 
      sequence-specific primer (SSP), sequence-specific oligonucleotide, and Sanger 
      sequencing on peripheral blood leukocytes and buccal cells, from a 3-month-old 
      boy and peripheral blood leukocytes from his parents. Short tandem repeat (STR) 
      testing was performed in parallel. RESULTS: HLA typing of the patient's 
      peripheral blood leukocytes using the SSP method demonstrated three different 
      alleles for each of the HLA-B and HLA-C loci, with both maternal alleles present 
      at each locus. Typing results from the patient's buccal cells showed a normal 
      pattern of inheritance for paternal and maternal haplotypes. STR enrichment 
      testing of the patient's CD3+ T lymphocytes and CD15+ myeloid cells confirmed 
      maternal T-cell engraftment, while the myeloid cell profile matched the patient's 
      buccal cells. CONCLUSIONS: Maternal T-cell engraftment may interfere with HLA 
      typing in patients with SCID. Selection of the appropriate typing methods and 
      specimens is critical for accurate HLA typing and immunologic assessment before 
      allogeneic hematopoietic stem cell transplantation.
CI  - (c) American Society for Clinical Pathology, 2016. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Liu, Chang
AU  - Liu C
AD  - From the Division of Laboratory and Genomic Medicine, Department of Pathology and 
      Immunology HLA Laboratory.
FAU - Duffy, Brian
AU  - Duffy B
AD  - HLA Laboratory.
FAU - Bednarski, Jeffrey J
AU  - Bednarski JJ
AD  - Department of Pediatrics, and.
FAU - Calhoun, Cecelia
AU  - Calhoun C
AD  - Department of Pediatrics, and.
FAU - Lay, Lindsay
AU  - Lay L
AD  - Molecular Diagnostic Laboratory, Barnes-Jewish Hospital, St Louis, MO.
FAU - Rundblad, Barrett
AU  - Rundblad B
AD  - Molecular Diagnostic Laboratory, Barnes-Jewish Hospital, St Louis, MO.
FAU - Payton, Jacqueline E
AU  - Payton JE
AD  - From the Division of Laboratory and Genomic Medicine, Department of Pathology and 
      Immunology Molecular Diagnostic Laboratory, Barnes-Jewish Hospital, St Louis, MO.
FAU - Mohanakumar, Thalachallour
AU  - Mohanakumar T
AD  - From the Division of Laboratory and Genomic Medicine, Department of Pathology and 
      Immunology HLA Laboratory Department of Surgery, Washington University School of 
      Medicine, St Louis, MO; and\
LA  - eng
GR  - K08 AI102946/AI/NIAID NIH HHS/United States
GR  - T32 HD007499/HD/NICHD NIH HHS/United States
PT  - Case Reports
PT  - Journal Article
DEP - 20160201
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
SB  - IM
MH  - Alleles
MH  - Chimerism
MH  - HLA Antigens/immunology
MH  - HLA-B Antigens/*genetics/immunology
MH  - HLA-C Antigens/*genetics/immunology
MH  - Haplotypes
MH  - Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunophenotyping
MH  - Infant
MH  - Leukocytes/immunology
MH  - Male
MH  - Microsatellite Repeats/genetics
MH  - Mouth Mucosa/pathology
MH  - Sequence Analysis, DNA
MH  - Severe Combined Immunodeficiency/*diagnosis/immunology/therapy
MH  - T-Lymphocytes/immunology/pathology
PMC - PMC4901308
MID - NIHMS790930
OTO - NOTNLM
OT  - HLA typing
OT  - Maternal T-cell engraftment
OT  - Sanger sequencing
OT  - Sequence-specific oligonucleotide
OT  - Sequence-specific primer
OT  - Severe combined immunodeficiency
OT  - Short tandem repeat
EDAT- 2016/02/03 06:00
MHDA- 2017/04/06 06:00
PMCR- 2017/02/01
CRDT- 2016/02/03 06:00
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2017/04/06 06:00 [medline]
PHST- 2017/02/01 00:00 [pmc-release]
AID - aqv079 [pii]
AID - 10.1093/ajcp/aqv079 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2016 Feb;145(2):251-7. doi: 10.1093/ajcp/aqv079. Epub 2016 Feb 
      1.