PMID- 26834744
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160202
LR  - 20181113
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 7
DP  - 2016
TI  - Induction of Monocyte Chemoattractant Proteins in Macrophages via the Production 
      of Granulocyte/Macrophage Colony-Stimulating Factor by Breast Cancer Cells.
PG  - 2
LID - 10.3389/fimmu.2016.00002 [doi]
LID - 2
AB  - Monocyte chemoattractant protein-1 (MCP-1)/CCL2 plays an important role in the 
      initiation and progression of cancer. We previously reported that in 4T1 murine 
      breast cancer, non-tumor stromal cells, including macrophages, were the major 
      source of MCP-1. In the present study, we analyzed the potential mechanisms by 
      which MCP-1 is upregulated in macrophages infiltrating 4T1 tumors. We found that 
      cell-free culture supernatants of 4T1 cells (4T1-sup) markedly upregulated MCP-1 
      production by peritoneal inflammatory macrophages. 4T1-sup also upregulated other 
      MCPs, such as MCP-3/CCL7 and MCP-5/CCL12, but modestly upregulated neutrophil 
      chemotactic chemokines, such as KC/CXCL1 or MIP-2/CXCL2. Physicochemical analysis 
      indicated that an approximately 20-30 kDa 4T1 cell product was responsible for 
      the capacity of 4T1-sup to upregulate MCP-1 expression by macrophages. A 
      neutralizing antibody against granulocyte/macrophage colony-stimulating factor 
      (GM-CSF), but not macrophage CSF, almost completely abrogated MCP-1-inducing 
      activity of 4T1-sup, and recombinant GM-CSF potently upregulated MCP-1 production 
      by macrophages. The expression levels of GM-CSF in 4T1 tumors in vivo were higher 
      than other tumors, such as Lewis lung carcinoma. Treatment of mice with 
      anti-GM-CSF antibody significantly reduced the growth of 4T1 tumors at the 
      injection sites but did not reduce MCP-1 production or lung metastasis in 
      tumor-bearing mice. These results indicate that 4T1 cells have the capacity to 
      directly upregulate MCP-1 production by macrophages by releasing GM-CSF; however, 
      other mechanisms are also involved in increased MCP-1 levels in the 4T1 tumor 
      microenvironment.
FAU - Yoshimura, Teizo
AU  - Yoshimura T
AD  - Cancer and Inflammation Program, Center for Cancer Research, National Cancer 
      Institute, Frederick, MD, USA; Department of Pathology and Experimental Medicine, 
      Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 
      University, Okayama, Japan.
FAU - Imamichi, Tomozumi
AU  - Imamichi T
AD  - Laboratory of Human Retrovirology and Immunoinformatics, Leidos Biomedical 
      Research, Inc., Frederick National Laboratory for Cancer Research , Frederick, MD 
      , USA.
FAU - Weiss, Jonathan M
AU  - Weiss JM
AD  - Cancer and Inflammation Program, Center for Cancer Research, National Cancer 
      Institute , Frederick, MD , USA.
FAU - Sato, Miwa
AU  - Sato M
AD  - Department of Pathology and Experimental Medicine, Graduate School of Medicine, 
      Dentistry and Pharmaceutical Sciences, Okayama University , Okayama , Japan.
FAU - Li, Liangzhu
AU  - Li L
AD  - Engineering Research Center for Cell and Therapeutic Antibody of Ministry of 
      Education, School of Pharmacy, Shanghai Jiaotong University , Shanghai , China.
FAU - Matsukawa, Akihiro
AU  - Matsukawa A
AD  - Department of Pathology and Experimental Medicine, Graduate School of Medicine, 
      Dentistry and Pharmaceutical Sciences, Okayama University , Okayama , Japan.
FAU - Wang, Ji Ming
AU  - Wang JM
AD  - Cancer and Inflammation Program, Center for Cancer Research, National Cancer 
      Institute , Frederick, MD , USA.
LA  - eng
GR  - HHSN261200800001C/RC/CCR NIH HHS/United States
GR  - HHSN261200800001E/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20160120
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
EIN - Front Immunol. 2017 Nov 13;8:1524. PMID: 29151836
PMC - PMC4718995
OTO - NOTNLM
OT  - breast cancer
OT  - chemokines
OT  - inflammation
OT  - monocytes/macrophages
OT  - tumor microenvironment
EDAT- 2016/02/03 06:00
MHDA- 2016/02/03 06:01
PMCR- 2016/01/01
CRDT- 2016/02/03 06:00
PHST- 2015/08/11 00:00 [received]
PHST- 2016/01/05 00:00 [accepted]
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2016/02/03 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2016.00002 [doi]
PST - epublish
SO  - Front Immunol. 2016 Jan 20;7:2. doi: 10.3389/fimmu.2016.00002. eCollection 2016.