PMID- 26834749
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160202
LR  - 20190109
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 7
DP  - 2016
TI  - Tolerogenic IDO(+) Dendritic Cells Are Induced by PD-1-Expressing Mast Cells.
PG  - 9
LID - 10.3389/fimmu.2016.00009 [doi]
LID - 9
AB  - Mast cells (MCs) are tissue resident cells, rich in inflammatory mediators, 
      involved in allergic reactions, and with an increasingly recognized role in 
      immunomodulation. Dendritic cells (DCs), on the other hand, are central to the 
      determination of immune response patterns, being highly efficient 
      antigen-presenting cells that respond promptly to changes in their 
      microenvironment. Here, we show that direct cell contact between immature 
      monocyte-derived DCs (iDCs) and MC bends DCs toward tolerance induction. DCs that 
      had direct contact with MC (MC-iDC) decreased HLA-DR but increased PD-L1 
      expression and stimulated regulatory T lymphocytes, which expresses FoxP3(+), 
      secrete TGF-beta and IL-10, and suppress the proliferation of mitogen-stimulated 
      naive T lymphocytes. Furthermore, MC-iDC expressed higher levels of 
      indoleamine-2,3-deoxigenase (IDO), a phenomenon that was blocked by treatment of 
      MC with anti-PD-1 or by the treatment of DCs with anti-PD-L1 or anti-PD-L2, but 
      not by blocking of H1 and H2 histamine receptors on DCs. Contact with MC also 
      increased phosphorylated STAT-3 levels in iDCs. When a STAT-3 inhibitor, JSI-124, 
      was added to the DCs before contact with MC, the MC-iDC recovered their ability 
      to induce allogeneic T cell proliferation and did not increase their IDO 
      expression.
FAU - Rodrigues, Cecilia Pessoa
AU  - Rodrigues CP
AD  - Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical 
      Sciences, University of Sao Paulo , Sao Paulo , Brazil.
FAU - Ferreira, Ana Carolina Franco
AU  - Ferreira AC
AD  - Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical 
      Sciences, University of Sao Paulo , Sao Paulo , Brazil.
FAU - Pinho, Mariana Pereira
AU  - Pinho MP
AD  - Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical 
      Sciences, University of Sao Paulo , Sao Paulo , Brazil.
FAU - de Moraes, Cristiano Jacob
AU  - de Moraes CJ
AD  - Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical 
      Sciences, University of Sao Paulo , Sao Paulo , Brazil.
FAU - Bergami-Santos, Patricia Cruz
AU  - Bergami-Santos PC
AD  - Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical 
      Sciences, University of Sao Paulo , Sao Paulo , Brazil.
FAU - Barbuto, Jose Alexandre Marzagao
AU  - Barbuto JA
AD  - Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical 
      Sciences, University of Sao Paulo, Sao Paulo, Brazil; Center for Cellular and 
      Molecular Studies and Therapy (NETCEM), University of Sao Paulo, Sao Paulo, 
      Brazil.
LA  - eng
PT  - Journal Article
DEP - 20160125
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC4724729
OTO - NOTNLM
OT  - IDO
OT  - STAT-3
OT  - Tregs
OT  - mast cells
OT  - tolerogenic DCs
EDAT- 2016/02/03 06:00
MHDA- 2016/02/03 06:01
PMCR- 2016/01/01
CRDT- 2016/02/03 06:00
PHST- 2015/11/10 00:00 [received]
PHST- 2016/01/11 00:00 [accepted]
PHST- 2016/02/03 06:00 [entrez]
PHST- 2016/02/03 06:00 [pubmed]
PHST- 2016/02/03 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2016.00009 [doi]
PST - epublish
SO  - Front Immunol. 2016 Jan 25;7:9. doi: 10.3389/fimmu.2016.00009. eCollection 2016.