PMID- 26837895
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 52
IP  - 2
DP  - 2016 Apr
TI  - Enhanced humoral and cellular immune responses to PRRS virus GP5 glycoprotein by 
      DNA prime-adenovirus boost vaccination in mice.
PG  - 228-34
LID - 10.1007/s11262-016-1293-2 [doi]
AB  - In order to investigate the induction of humoral and cellular immune responses 
      against porcine reproductive and respiratory syndrome virus (PRRSV), BALB/c mice 
      were immunized in a pcDNA3-GP5 prime-rAd-GP5 boost regimen. After humoral and 
      cellular immune response detection, levels of PRRSV-specific antibodies, 
      neutralizing antibodies, lymphocyte proliferation response, and cytotoxic 
      T-lymphocyte response were significantly increased as compared to controls. The 
      humoral immune response was induced more effectively by the DNA priming and 
      recombinant adenovirus boosting regimen. Significant difference was observed 
      between heterogeneous and homologous vaccination. Induction of anti-GP5 antibody 
      response was higher in all heterogeneous combinations than those of the 
      homologous combinations. In the induction of lymphocyte proliferation response 
      and CTL response, the homologous combination of 
      pcDNA3-GP5/pcDNA3-GP5/pcDNA3-GP5was significantly stronger than that of 
      rAd-GP5/rAd-GP5/rAd-GP5, but was relatively weaker than the heterogeneous 
      combination of pcDNA3-GP5/pcDNA3-GP5/rAd-GP5 and pcDNA3-GP5/rAd-GP5/rAd-GP5. This 
      heterogeneous combination was a most efficient immunization regimen in induction 
      of PRRSV-specific cellular immune response just as the antibody response. These 
      results suggested that DNA immunization followed by recombinant adenovirus 
      boosting could be used as a potential PRRSV vaccine.
FAU - Yu, Meifang
AU  - Yu M
AD  - China Animal Health and Epidemiology Center, No. 369 Nanjing Road, Qingdao, 
      266032, People's Republic of China.
FAU - Qiu, Yuan
AU  - Qiu Y
AD  - China Animal Health and Epidemiology Center, No. 369 Nanjing Road, Qingdao, 
      266032, People's Republic of China.
FAU - Chen, Jiming
AU  - Chen J
AD  - China Animal Health and Epidemiology Center, No. 369 Nanjing Road, Qingdao, 
      266032, People's Republic of China.
FAU - Jiang, Wenming
AU  - Jiang W
AD  - China Animal Health and Epidemiology Center, No. 369 Nanjing Road, Qingdao, 
      266032, People's Republic of China. civcul@163.com.
LA  - eng
PT  - Journal Article
DEP - 20160202
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Adenoviridae/genetics/immunology
MH  - Animals
MH  - Antibodies, Neutralizing
MH  - Antibodies, Viral
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - *Immunity, Cellular
MH  - *Immunity, Humoral
MH  - Immunization
MH  - Immunization, Secondary
MH  - Immunoglobulin G/immunology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Vaccines, DNA/*immunology
MH  - Viral Envelope Proteins/genetics/*immunology
MH  - Viral Vaccines/genetics/*immunology
OTO - NOTNLM
OT  - DNA vaccine
OT  - GP5 glycoprotein
OT  - PRRSV
OT  - Recombinant adenovirus
EDAT- 2016/02/04 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/04 06:00
PHST- 2015/11/02 00:00 [received]
PHST- 2016/01/12 00:00 [accepted]
PHST- 2016/02/04 06:00 [entrez]
PHST- 2016/02/04 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1007/s11262-016-1293-2 [pii]
AID - 10.1007/s11262-016-1293-2 [doi]
PST - ppublish
SO  - Virus Genes. 2016 Apr;52(2):228-34. doi: 10.1007/s11262-016-1293-2. Epub 2016 Feb 
      2.