PMID- 26838339
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20170406
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 26
IP  - 5
DP  - 2016 May 28
TI  - Cell Surface Display of Four Types of Solanum nigrum Metallothionein on 
      Saccharomyces cerevisiae for Biosorption of Cadmium.
PG  - 846-53
LID - 10.4014/jmb.1512.12041 [doi]
AB  - We displayed four types of Solanum nigrum metallothionein (SMT) for the first 
      time on the surface of Saccharomyces cerevisiae using an alpha-agglutinin-based 
      display system. The SMT genes were amplified by RT-PCR. The plasmid pYES2 was 
      used to construct the expression vector. Transformed yeast strains were confirmed 
      by PCR amplification and custom sequencing. Surface-expressed metallothioneins 
      were indirectly indicated by the enhanced cadmium sorption capacity. Flame atomic 
      absorption spectrophotometry was used to examine the concentration of Cd(2+) in 
      this study. The transformed yeast strains showed much higher resistance ability 
      to Cd(2+) compared with the control. Strikingly, their Cd(2+) accumulation was 
      almost twice as much as that of the wild-type yeast cells. Furthermore, 
      surface-engineered yeast strains could effectively adsorb ultra-trace cadmium and 
      accumulate Cd(2+) under a wide range of pH levels, from 3 to 7, without 
      disturbing the Cu(2+) and Hg(2+). Four types of surfaceengineered Saccharomyces 
      cerevisiae strains were constructed and they could be used to purify 
      Cd(2+)-contaminated water and adsorb ultra-trace cadmium effectively. The 
      surface-engineered Saccharomyces cerevisiae strains would be useful tools for the 
      bioremediation and biosorption of environmental cadmium contaminants.
FAU - Wei, Qinguo
AU  - Wei Q
AD  - College of Life Science, Qufu Normal University, Qufu, Shandong 273165, P.R. 
      China.
AD  - Department of Biotechnology, Weifang Business Vocational College, Zhucheng, 
      Shandong 262234, P.R. China.
FAU - Zhang, Honghai
AU  - Zhang H
AD  - College of Life Science, Qufu Normal University, Qufu, Shandong 273165, P.R. 
      China.
FAU - Guo, Dongge
AU  - Guo D
AD  - College of Life Science, Qufu Normal University, Qufu, Shandong 273165, P.R. 
      China.
FAU - Ma, Shisheng
AU  - Ma S
AD  - College of Life Science, Qufu Normal University, Qufu, Shandong 273165, P.R. 
      China.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (DNA, Complementary)
RN  - 0 (Plant Proteins)
RN  - 0 (Trace Elements)
RN  - 00BH33GNGH (Cadmium)
RN  - 9038-94-2 (Metallothionein)
SB  - IM
MH  - Adsorption
MH  - Biodegradation, Environmental
MH  - Cadmium/*metabolism
MH  - Cell Membrane/chemistry/metabolism
MH  - DNA, Complementary/genetics
MH  - Escherichia coli/genetics
MH  - Genes, Plant
MH  - Genetic Engineering/methods
MH  - Metallothionein/*biosynthesis/*genetics/metabolism
MH  - Plant Proteins/biosynthesis/chemistry/genetics
MH  - Plasmids/genetics
MH  - Saccharomyces cerevisiae/*genetics/*metabolism
MH  - Solanum nigrum/chemistry/*genetics/*metabolism
MH  - Surface Properties
MH  - Trace Elements/chemistry
OTO - NOTNLM
OT  - Cadmium
OT  - Metallothionein
OT  - Saccharomyces cerevisiae
OT  - Solanum nigrum
OT  - Surface display
OT  - flame atomic absorption spectrophotometer(FAAS)
EDAT- 2016/02/04 06:00
MHDA- 2017/04/07 06:00
CRDT- 2016/02/04 06:00
PHST- 2016/02/04 06:00 [entrez]
PHST- 2016/02/04 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
AID - 10.4014/jmb.1512.12041 [pii]
AID - 10.4014/jmb.1512.12041 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2016 May 28;26(5):846-53. doi: 10.4014/jmb.1512.12041.