PMID- 26838421
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 150
DP  - 2016 Apr 1
TI  - Polydopamine-assisted partial hydrolyzed poly(2-methyl-2-oxazolinze) as coating 
      for determination of melamine in milk by capillary electrophoresis.
PG  - 375-87
LID - S0039-9140(15)30578-6 [pii]
LID - 10.1016/j.talanta.2015.12.054 [doi]
AB  - Nitrogen-rich melamine (66% by mass) had been found to be illegally adulterated 
      to milk products and animal feed in order to increase the apparent protein 
      content, and ingestion of melamine may lead to the formation of kidney stones. 
      Hence, the development of reliable analytical methods for the monitoring of 
      melamine in milk products is necessary. The common methods for melamine analysis 
      in milk products are performed via GC-MS or HPLC, however, these procedures 
      require a large of organic solvent and several pretreatment steps. In this study, 
      a new and facile approach was developed to modify a fused-silica capillary inner 
      surface based on poly(dopamine) (PDA) and partial hydrolyzed 
      poly(2-methyl-2-oxazoline) (PMOXA-EI) mixed coating, which was used to determine 
      the melamine in milk powder quantitatively by capillary electrophoresis with 
      ultraviolet (CE-UV) detection with simple analytes pretreatment. Firstly, the 
      influences of the molecular mass of poly(2-methyl-2-oxazoline) (PMOXA), the 
      hydrolysis degree of PMOXA, and the concentration ratio of DA to PMOXA-EI on the 
      separation properties of a mixture of four basic proteins (cytochrome c, 
      lysozyme, ribonuclease A, and alpha-chymotrypsinogen A) were studied in detail. As a 
      consequence, fast and efficient separation of four proteins was obtained using 
      PDA/PMOXA1k-EI0.4 coating (once coating). Then, a modified facile coating 
      workflow was carried out to acquire a novel twice coating (i.e. 
      PDA/PMOXA1k-EI0.4+PMOXA1k-EI0.4 coated capillary) for highly sensitive and stable 
      analysis of melamine. Under the optimal conditions, the linear response of 
      melamine concentration ranged from 6.25 to 100 microg/mL with high correlation 
      coefficient of 0.9910, and the limit of detection (LOD) for melamine was 0.097 
      microg/mL. The minimum amount of melamine determined in milk powder with our proposed 
      method was 4 mg/kg. The recoveries and relative standard deviations (RSDs) were 
      92.13-102.47%, 92.22-100.30%, 2.07-4.98%, and 2.43-3.77%, respectively for the 
      intraday and interday analysis. After being used continually for 45 days, the 
      PDA/PMOXA1k-EI0.4+PMOXA1k-EI0.4 coated capillary could be still used to detect 
      the melamine from milk powder, with the recovery from 91.85% to 106.14%. The 
      technique developed in this study provides a simple and relatively sensitive 
      method for determination of melamine in milk powder.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Zhang, Yalin
AU  - Zhang Y
AD  - CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and 
      Engineering, University of Science and Technology of China, Hefei 230026, PR 
      China.
FAU - Chen, Lijuan
AU  - Chen L
AD  - CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and 
      Engineering, University of Science and Technology of China, Hefei 230026, PR 
      China.
FAU - Zhang, Chong
AU  - Zhang C
AD  - CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and 
      Engineering, University of Science and Technology of China, Hefei 230026, PR 
      China.
FAU - Liu, Songtao
AU  - Liu S
AD  - CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and 
      Engineering, University of Science and Technology of China, Hefei 230026, PR 
      China.
FAU - Zhu, Haikun
AU  - Zhu H
AD  - CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and 
      Engineering, University of Science and Technology of China, Hefei 230026, PR 
      China.
FAU - Wang, Yanmei
AU  - Wang Y
AD  - CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and 
      Engineering, University of Science and Technology of China, Hefei 230026, PR 
      China. Electronic address: wangyanm@ustc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151221
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Buffers)
RN  - 0 (Indoles)
RN  - 0 (Polyamines)
RN  - 0 (Polymers)
RN  - 0 (Triazines)
RN  - 0 (poly(2-methyl-2-oxazoline))
RN  - 0 (polydopamine)
RN  - N3GP2YSD88 (melamine)
SB  - IM
MH  - Animals
MH  - Buffers
MH  - Electric Conductivity
MH  - Electrophoresis, Capillary/methods
MH  - Food Contamination/*analysis
MH  - Hydrogen-Ion Concentration
MH  - Hydrolysis
MH  - Indoles/*chemistry
MH  - Limit of Detection
MH  - Milk/*chemistry
MH  - Polyamines/*chemistry
MH  - Polymers/*chemistry
MH  - Reproducibility of Results
MH  - Triazines/*analysis/*isolation & purification
OTO - NOTNLM
OT  - Capillary electrophoresis
OT  - Melamine
OT  - Milk powder.
OT  - Poly(2-methyl-2-oxazoline-co-ethyleneimine)
OT  - Poly(dopamine)
EDAT- 2016/02/04 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/04 06:00
PHST- 2015/09/12 00:00 [received]
PHST- 2015/12/19 00:00 [revised]
PHST- 2015/12/19 00:00 [accepted]
PHST- 2016/02/04 06:00 [entrez]
PHST- 2016/02/04 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0039-9140(15)30578-6 [pii]
AID - 10.1016/j.talanta.2015.12.054 [doi]
PST - ppublish
SO  - Talanta. 2016 Apr 1;150:375-87. doi: 10.1016/j.talanta.2015.12.054. Epub 2015 Dec 
      21.