PMID- 26838907
OWN - NLM
STAT- MEDLINE
DCOM- 20161028
LR  - 20161230
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 61
DP  - 2016 Apr 1
TI  - Fluorescence enhancement of glutaraldehyde functionalized polyaniline nanofibers 
      in the presence of aromatic amino acids.
PG  - 762-72
LID - S0928-4931(16)30008-X [pii]
LID - 10.1016/j.msec.2016.01.010 [doi]
AB  - Polyaniline nanofibers (PNFs) synthesized by dilute polymerization method have 
      been surface functionalized with glutaraldehyde at their N-terminals in Phosphate 
      Buffered Saline (PBS) at P(H)=7.4 in order to achieve improved interaction of 
      surface functionalized polyaniline nanofibers (SF-PNFs) with aromatic amino 
      acids-Tyrosine, Tryptophan and Phenylalanine through incorporation of aldehyde 
      (-CHO) and hydroxyl (-OH) functionalities. HRTEM reveals nanofibers of average 
      diameter of 35.66 nm. FESEM depicts interconnected networks of nanofibers of 
      polyaniline (PAni). UV-visible absorption and Fluorescence spectroscopy indicate 
      that the PNFs and SF-PNFs are in emeraldine base (EB) form. FT-IR, (1)H NMR 
      spectroscopy suggests covalent interactions of SF-PNFs with aromatic amino acids 
      and possible reaction mechanisms have been proposed based on these results. 
      Remarkable enhancement in fluorescence signals of SF-PNFs in the presence of 
      aromatic amino acids has been observed and the apparent binding constant (KA) and 
      the number of binding sites (n) have been calculated using fluorescence 
      enhancement equation. The KA value is found to be highest for SF-PNFs+Tyrosine 
      and n is two for all the polymer amino acid complexes, which are in agreement 
      with the FT-IR and (1)H NMR results. Fluorescence resonance energy transfer 
      (FRET) efficiency has been found to be highest for SF-PNFs+Tyrosine giving 
      maximum fluorescence enhancement. The study of interaction mechanisms by means of 
      an extremely sensitive technique like fluorescence using SF-PNFs as a substrate 
      may provide a promising analytical tool for detection and monitoring any 
      biochemical reactions involving these three aromatic amino acids.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Borah, Rajiv
AU  - Borah R
AD  - Materials Research Laboratory, Department of Physics, Tezpur University, Tezpur 
      784028, India.
FAU - Kumar, Ashok
AU  - Kumar A
AD  - Materials Research Laboratory, Department of Physics, Tezpur University, Tezpur 
      784028, India. Electronic address: ask@tezu.ernet.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160108
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Amino Acids, Aromatic)
RN  - 0 (Aniline Compounds)
RN  - 0 (polyaniline)
RN  - 42HK56048U (Tyrosine)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - Amino Acids, Aromatic/chemistry
MH  - Aniline Compounds/*chemistry
MH  - Fluorescence Resonance Energy Transfer
MH  - Glutaral/*chemistry
MH  - Magnetic Resonance Spectroscopy
MH  - Nanofibers/*chemistry
MH  - Spectrophotometry, Ultraviolet
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Tyrosine/chemistry
OTO - NOTNLM
OT  - Enhancement
OT  - FRET
OT  - Fluorescence
OT  - Interaction
OT  - SF-PNFs
EDAT- 2016/02/04 06:00
MHDA- 2016/11/01 06:00
CRDT- 2016/02/04 06:00
PHST- 2015/09/30 00:00 [received]
PHST- 2015/12/09 00:00 [revised]
PHST- 2016/01/03 00:00 [accepted]
PHST- 2016/02/04 06:00 [entrez]
PHST- 2016/02/04 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
AID - S0928-4931(16)30008-X [pii]
AID - 10.1016/j.msec.2016.01.010 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2016 Apr 1;61:762-72. doi: 
      10.1016/j.msec.2016.01.010. Epub 2016 Jan 8.