PMID- 26840189
OWN - NLM
STAT- MEDLINE
DCOM- 20160801
LR  - 20240413
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 49
IP  - 1
DP  - 2016 Feb
TI  - Overexpression of CD61 promotes hUC-MSC differentiation into male germ-like 
      cells.
PG  - 36-47
LID - 10.1111/cpr.12236 [doi]
AB  - OBJECTIVES: Previous studies have shown that germ-like cells can be induced from 
      human umbilical cord mesenchymal stem cell (hUC-MSCs) in vitro. However, 
      induction efficiency was low and a stable system had not been built. CD61, also 
      called integrin-beta3, plays a significant role in cell differentiation, in that 
      CD61-positive-cell-derived pluripotent stem cells easily differentiate into 
      primordial germ-like cells (PGC). Here, we have explored whether overexpression 
      of CD61 would promote hUC-MSC differentiation into PGC and male germ-like cells. 
      MATERIALS AND METHODS: hUC-MSCs were cultured and transduced using 
      pCD61-CAGG-TRIP-pur (oCD61) and pTRIP-CAGG plasmid (Control), and hUC-MSCs 
      overexpressed CD61 were induced by bone morphogenetic protein 4 (BMP4, 12.5 
      ng/ml), to differentiate into PGC and male germ cells. Quantitative real-time PCR 
      (RT-qPCR), western blotting and immunofluorescence staining were used to examine 
      PGC- and germ cell-specific markers. RESULTS: High expression levels of 
      PGC-specific markers were detected in oCD61 hUC-MSCs compared to controls. After 
      BMP4 induction, expression levels of male germ cell markers such as Acrosin 
      (ACR), Prm1 and meiotic markers including Stra8, Scp3 in oCD61 were significantly 
      higher than those of the Control group. CONCLUSIONS: Under induction of BMP4, 
      CD61-overexpressing hUC-MSCs, which had turned into PGC-like cells, could be 
      further differentiated into male germ-like cells. Thus, a simple and efficient 
      approach to study male germ cell development by using hUC-MSCs has been 
      established.
CI  - (c) 2016 John Wiley & Sons Ltd.
FAU - Li, Bo
AU  - Li B
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & 
      Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Liu, Weishuai
AU  - Liu W
AD  - Department of Pathology, Yangling Demonstration Zone Hospital, Yangling, Shaanxi, 
      712100, China.
FAU - Zhuang, Mengru
AU  - Zhuang M
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & 
      Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Li, Na
AU  - Li N
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & 
      Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Wu, Siyu
AU  - Wu S
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & 
      Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Pan, Shaohui
AU  - Pan S
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & 
      Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Hua, Jinlian
AU  - Hua J
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & 
      Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
LA  - eng
GR  - L30 AA021605/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160203
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Biomarkers)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Codon)
RN  - 0 (Integrin beta3)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Biomarkers/metabolism
MH  - Bone Morphogenetic Protein 4/pharmacology
MH  - *Cell Differentiation
MH  - Cloning, Molecular
MH  - Codon/genetics
MH  - Germ Cells/*cytology
MH  - Humans
MH  - Integrin beta3/chemistry/genetics/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Protein Structure, Secondary
MH  - Sequence Analysis, Protein
MH  - Sequence Homology, Nucleic Acid
MH  - Umbilical Cord/*cytology
PMC - PMC6496844
EDAT- 2016/02/04 06:00
MHDA- 2016/08/02 06:00
PMCR- 2016/02/03
CRDT- 2016/02/04 06:00
PHST- 2015/08/24 00:00 [received]
PHST- 2015/10/06 00:00 [accepted]
PHST- 2016/02/04 06:00 [entrez]
PHST- 2016/02/04 06:00 [pubmed]
PHST- 2016/08/02 06:00 [medline]
PHST- 2016/02/03 00:00 [pmc-release]
AID - CPR12236 [pii]
AID - 10.1111/cpr.12236 [doi]
PST - ppublish
SO  - Cell Prolif. 2016 Feb;49(1):36-47. doi: 10.1111/cpr.12236. Epub 2016 Feb 3.