PMID- 26840352
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20190221
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 31
IP  - 1
DP  - 2016 Jan
TI  - Effect of folic acid in a modified experimental model of anorectal malformations 
      adriamycin-induced in rats.
PG  - 22-7
LID - S0102-86502016000100022 [pii]
LID - 10.1590/S0102-865020160010000004 [doi]
AB  - PURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce 
      anorectal malformations (ARMs) and determine the effect of folic acid (FA) in 
      this model. METHODS: Ten female Wistar rats were divided randomly in two groups. 
      Group A - ADR; Group B - FA+ADR. Dams from group B received daily, since two 
      weeks before the pregnancy to the end of pregnancy, FA (50mg/kg) by gavage. Dams 
      from both groups received ADR (6mk/kg) by intraperitoneal injection on 
      gestational day (GD) 8. Their fetuses were harvested by cesarean section on GD21 
      and were examined looking for ARMs. The thickness of anal stratified squamous 
      epithelium (ASSE) and intestinal epithelium (IE) were analyzed. p</=0.05*. RESULTS: 
      81 fetuses were harvested. The number of fetuses; number of ARMs; mean (∆%) (+/- 
      SD) were determined to be, respectively: ADR - 41[29;65%(+/-37%)] versus FA+ADR - 
      40[04;16%(+/-36%)] (p=0.05). AMRs were significantly lower in FA+ADR group than in 
      ADR group (p=0.05). The thickness (microm) of ASSE (+/- SD) and IE (+/- SD) were 
      measured, respectively: ADR - [25.98(+/-0.74) and 19.48(+/-1.68)] versus FA+ADR - 
      [24.74(+/-0.91) and 24.80(+/-0.81)] (p<0.005). The thickness of IE was significantly 
      enlarged when FA was given (p<0.005). CONCLUSIONS: Single dose of adriamycin on 
      D8 was able to induce anorectal malformations. Folic acid reduces the number and 
      enlarged the IE of ARMs ADR-induced.
FAU - Faria, Danilo Jose Fiorindo
AU  - Faria DJ
AD  - Universidade Federal de Sao Paulo, Brazil.
FAU - Simoes, Manuel de Jesus
AU  - Simoes Mde J
AD  - Department of Morphology, UNIFESP, Sao Paulo, SP, Brazil.
FAU - Teixeira, Luciana Cristina
AU  - Teixeira LC
AD  - UNIFESP, Sao Paulo, SP, Brazil.
FAU - Faria, Angela Tucci Pinheiro
AU  - Faria AT
AD  - Universidade Sao Judas Tadeu, Sao Paulo, SP, Brazil.
FAU - Cintra, Alvaro Edmundo Simoes Ulhoa
AU  - Cintra AE
AD  - UNIFESP, Sao Paulo, SP, Brazil.
FAU - Martins, Jose Luiz
AU  - Martins JL
AD  - Department of Pediatric Surgery, UNIFESP, Sao Paulo, SP, Brazil.
LA  - eng
PT  - Journal Article
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
RN  - 0 (Topoisomerase II Inhibitors)
RN  - 80168379AG (Doxorubicin)
RN  - 935E97BOY8 (Folic Acid)
SB  - IM
MH  - Animals
MH  - Anorectal Malformations
MH  - Anus, Imperforate/chemically induced/pathology/*prevention & control
MH  - Disease Models, Animal
MH  - Doxorubicin
MH  - Epithelium/abnormalities/pathology
MH  - Female
MH  - Fetus/abnormalities
MH  - Folic Acid/*administration & dosage
MH  - Pregnancy
MH  - Random Allocation
MH  - Rats, Wistar
MH  - Topoisomerase II Inhibitors
EDAT- 2016/02/04 06:00
MHDA- 2016/09/16 06:00
CRDT- 2016/02/04 06:00
PHST- 2015/09/15 00:00 [received]
PHST- 2015/12/19 00:00 [accepted]
PHST- 2016/02/04 06:00 [entrez]
PHST- 2016/02/04 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
AID - S0102-86502016000100022 [pii]
AID - 10.1590/S0102-865020160010000004 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2016 Jan;31(1):22-7. doi: 10.1590/S0102-865020160010000004.