PMID- 26840462
OWN - NLM
STAT- MEDLINE
DCOM- 20160712
LR  - 20190222
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 2
DP  - 2016
TI  - Alterations of the Ileal and Colonic Mucosal Microbiota in Canine Chronic 
      Enteropathies.
PG  - e0147321
LID - 10.1371/journal.pone.0147321 [doi]
LID - e0147321
AB  - BACKGROUND: The intestinal microbiota is increasingly linked to the pathogenesis 
      of chronic enteropathies (CE) in dogs. While imbalances in duodenal and fecal 
      microbial communities have been associated with mucosal inflammation, relatively 
      little is known about alterations in mucosal bacteria seen with CE involving the 
      ileum and colon. AIM: To investigate the composition and spatial organization of 
      mucosal microbiota in dogs with CE and controls. METHODS: Tissue sections from 
      endoscopic biopsies of the ileum and colon from 19 dogs with inflammatory bowel 
      disease (IBD), 6 dogs with granulomatous colitis (GC), 12 dogs with intestinal 
      neoplasia, and 15 controls were studied by fluorescence in situ hybridization 
      (FISH) on a quantifiable basis. RESULTS: The ileal and colonic mucosa of healthy 
      dogs and dogs with CE is predominantly colonized by bacteria localized to free 
      and adherent mucus compartments. CE dogs harbored more (P < 0.05) mucosal 
      bacteria belonging to the Clostridium-coccoides/Eubacterium rectale group, 
      Bacteroides, Enterobacteriaceae, and Escherichia coli versus controls. Within the 
      CE group, IBD dogs had increased (P < 0.05) Enterobacteriaceae and E. coli 
      bacteria attached onto surface epithelia or invading within the intestinal 
      mucosa. Bacterial invasion with E. coli was observed in the ileal and colonic 
      mucosa of dogs with GC (P < 0.05). Dogs with intestinal neoplasia had increased 
      (P < 0.05) adherent (total bacteria, Enterobacteriaceae, E. coli) and invasive 
      (Enterobacteriaceae, E. coli, and Bacteroides) bacteria in biopsy specimens. 
      Increased numbers of total bacteria adherent to the colonic mucosa were 
      associated with clinical disease severity in IBD dogs (P < 0.05). CONCLUSION: 
      Pathogenic events in canine CE are associated with different populations of the 
      ileal and colonic mucosal microbiota.
FAU - Cassmann, Eric
AU  - Cassmann E
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa 
      State University, Ames, Iowa, United States of America.
FAU - White, Robin
AU  - White R
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa 
      State University, Ames, Iowa, United States of America.
FAU - Atherly, Todd
AU  - Atherly T
AD  - USDA-ARS, Ames, Iowa, United States of America.
FAU - Wang, Chong
AU  - Wang C
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.
FAU - Sun, Yaxuan
AU  - Sun Y
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.
FAU - Khoda, Samir
AU  - Khoda S
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa 
      State University, Ames, Iowa, United States of America.
FAU - Mosher, Curtis
AU  - Mosher C
AD  - Department of Genetics, Development & Cell Biology, College of Liberal Arts and 
      Sciences, Iowa State University, Ames, Iowa, United States of America.
FAU - Ackermann, Mark
AU  - Ackermann M
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State 
      University, Ames, Iowa, United States of America.
FAU - Jergens, Albert
AU  - Jergens A
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa 
      State University, Ames, Iowa, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20160203
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Bacterial Load
MH  - Biopsy
MH  - Case-Control Studies
MH  - Chronic Disease
MH  - Colon/*microbiology/pathology
MH  - Dog Diseases/diagnosis/*microbiology/pathology
MH  - Dogs
MH  - Endoscopes, Gastrointestinal
MH  - *Gastrointestinal Microbiome
MH  - Ileum/*microbiology/pathology
MH  - In Situ Hybridization, Fluorescence
MH  - Intestinal Diseases/*veterinary
MH  - Intestinal Mucosa/*microbiology/pathology
MH  - Severity of Illness Index
PMC - PMC4740465
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/02/04 06:00
MHDA- 2016/07/13 06:00
PMCR- 2016/02/03
CRDT- 2016/02/04 06:00
PHST- 2015/02/27 00:00 [received]
PHST- 2015/12/31 00:00 [accepted]
PHST- 2016/02/04 06:00 [entrez]
PHST- 2016/02/04 06:00 [pubmed]
PHST- 2016/07/13 06:00 [medline]
PHST- 2016/02/03 00:00 [pmc-release]
AID - PONE-D-15-08828 [pii]
AID - 10.1371/journal.pone.0147321 [doi]
PST - epublish
SO  - PLoS One. 2016 Feb 3;11(2):e0147321. doi: 10.1371/journal.pone.0147321. 
      eCollection 2016.