PMID- 26840919
OWN - NLM
STAT- MEDLINE
DCOM- 20180228
LR  - 20191210
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 45
IP  - 1
DP  - 2016 Mar
TI  - Validation of an automated enzyme immunoassay for the measurement of serum total 
      thyroxine in cats.
PG  - 148-53
LID - 10.1111/vcp.12324 [doi]
AB  - BACKGROUND: Hyperthyroidism is common in older cats, which necessitates frequent 
      screening of serum total thyroxine (TT4) concentrations. Fast, cheap, and 
      reliable methods to measure TT4 in cats are needed. OBJECTIVES: The purpose of 
      the study was the validation of a human TT4 enzyme immunoassay (EIA) for use with 
      feline serum, and derivation of a TT4 reference interval (RI) for cats aged 9 
      years and older. METHODS: Assay precision, reproducibility, and linearity were 
      evaluated. Interference by hemolysis was also assessed. Method comparison studies 
      between the human EIA and a previously validated radioimmunoassay (RIA) and 
      chemiluminescent-enzyme immunoassay (CEIA) were performed. Healthy cats (> 9 
      years) were recruited from 3 UK first opinion practices. RESULTS: The human TT4 
      EIA demonstrated good precision and reproducibility, and adequate linearity. 
      Hemolysis did not significantly alter measured TT4 concentrations until HGB > 8 
      g/L. Method comparison revealed proportional and constant errors between EIA and 
      RIA/CEIA. The TT4 RI for cats (> 9 years) was calculated as 7.1-45.1 nmol/L (n = 
      49). CONCLUSIONS: The human TT4 EIA was successfully validated for use with 
      feline serum and offers a rapid, cheap, and reliable method for determination of 
      serum TT4 concentrations in cats.
CI  - (c) 2016 American Society for Veterinary Clinical Pathology.
FAU - Williams, Tim L
AU  - Williams TL
AD  - Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
FAU - Archer, Joy
AU  - Archer J
AD  - Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20160203
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
RN  - Q51BO43MG4 (Thyroxine)
SB  - IM
MH  - Animals
MH  - Cat Diseases/*diagnosis
MH  - Cats
MH  - Female
MH  - Hyperthyroidism/diagnosis/*veterinary
MH  - Immunoenzyme Techniques/*veterinary
MH  - Male
MH  - Radioimmunoassay/veterinary
MH  - Reference Values
MH  - Reproducibility of Results
MH  - Thyroid Function Tests/veterinary
MH  - Thyroxine/*blood
OTO - NOTNLM
OT  - Analyzer
OT  - feline
OT  - human
OT  - hyperthyroidism
OT  - reference interval
EDAT- 2016/02/04 06:00
MHDA- 2018/03/01 06:00
CRDT- 2016/02/04 06:00
PHST- 2016/02/04 06:00 [entrez]
PHST- 2016/02/04 06:00 [pubmed]
PHST- 2018/03/01 06:00 [medline]
AID - 10.1111/vcp.12324 [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2016 Mar;45(1):148-53. doi: 10.1111/vcp.12324. Epub 2016 Feb 3.