PMID- 26844926
OWN - NLM
STAT- MEDLINE
DCOM- 20170705
LR  - 20221207
IS  - 1535-4970 (Electronic)
IS  - 1073-449X (Linking)
VI  - 194
IP  - 2
DP  - 2016 Jul 15
TI  - Allergen-induced Changes in Bone Marrow and Airway Dendritic Cells in Subjects 
      with Asthma.
PG  - 169-77
LID - 10.1164/rccm.201508-1623OC [doi]
AB  - RATIONALE: Dendritic cells (DCs) are antigen-presenting cells essential for the 
      initiation of T-cell responses. Allergen inhalation increases the number of 
      airway DCs and the release of epithelial-derived cytokines, such as IL-33 and 
      thymic stromal lymphopoietin (TSLP), that activate DCs. OBJECTIVES: To examine 
      the effects of inhaled allergen on bone marrow production of DCs and their 
      trafficking into the airways in subjects with allergic asthma, and to examine 
      IL-33 and TSPL receptor expression on DCs. METHODS: Bone marrow, peripheral 
      blood, bronchoalveolar lavage (BAL), and bronchial biopsies were obtained before 
      and after inhalation of diluent and allergen from subjects with asthma that 
      develop allergen-induced dual responses. Classical DCs (cDCs) were cultured from 
      bone marrow CD34(+) cells. cDC1s, cDC2s, and plasmacytoid DCs were measured in 
      bone marrow aspirates, peripheral blood, and BAL by flow cytometry, and cDCs were 
      quantified in bronchial biopsies by immunofluorescence staining. MEASUREMENTS AND 
      MAIN RESULTS: Inhaled allergen increased the number of cDCs grown from bone 
      marrow progenitors, and cDCs and plasmacytoid DCs in bone marrow aspirates 24 
      hours after allergen. Allergen also increased the expression of the TSLP 
      receptor, but not the IL-33 receptor, on bone marrow DCs. Finally, inhaled 
      allergen increased the percentage of cDC1s and cDC2s in BAL but only cDC2s in 
      bronchial tissues. CONCLUSIONS: Inhaled allergen increases DCs in bone marrow and 
      trafficking of DCs into the airway, which is associated with the development 
      airway inflammation in subjects with allergic asthma. Inhaled allergen challenge 
      also increases expression of TSLP, but not IL-33, receptors on bone marrow DCs.
FAU - El-Gammal, Amani
AU  - El-Gammal A
AD  - 1 Firestone Institute of Respiratory Health.
AD  - 2 Department of Medicine, and.
FAU - Oliveria, John-Paul
AU  - Oliveria JP
AD  - 2 Department of Medicine, and.
FAU - Howie, Karen
AU  - Howie K
AD  - 2 Department of Medicine, and.
FAU - Watson, Richard
AU  - Watson R
AD  - 2 Department of Medicine, and.
FAU - Mitchell, Patrick
AU  - Mitchell P
AD  - 2 Department of Medicine, and.
FAU - Chen, Ruchong
AU  - Chen R
AD  - 1 Firestone Institute of Respiratory Health.
AD  - 3 Guangzhou Institute of Respiratory Disease, 1st Affiliated Hospital of 
      Guangzhou Medical University, Guangzhou, China.
FAU - Baatjes, Adrian
AU  - Baatjes A
AD  - 2 Department of Medicine, and.
FAU - Smith, Steven
AU  - Smith S
AD  - 2 Department of Medicine, and.
FAU - Al-Sajee, Dhuha
AU  - Al-Sajee D
AD  - 4 Department of Pathology and Molecular Medicine, Michael G. DeGroote School of 
      Medicine, McMaster University, Hamilton, Ontario, Canada; and.
FAU - Hawke, Thomas J
AU  - Hawke TJ
AD  - 4 Department of Pathology and Molecular Medicine, Michael G. DeGroote School of 
      Medicine, McMaster University, Hamilton, Ontario, Canada; and.
FAU - Killian, Kieran J
AU  - Killian KJ
AD  - 2 Department of Medicine, and.
FAU - Gauvreau, Gail M
AU  - Gauvreau GM
AD  - 2 Department of Medicine, and.
FAU - O'Byrne, Paul M
AU  - O'Byrne PM
AD  - 1 Firestone Institute of Respiratory Health.
AD  - 2 Department of Medicine, and.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-33)
RN  - GT0IL38SP4 (Thymic Stromal Lymphopoietin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Allergens/*immunology/metabolism
MH  - Asthma/*immunology/metabolism
MH  - Bone Marrow/*immunology/metabolism
MH  - Cytokines/immunology/metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-33/immunology/metabolism
MH  - Male
MH  - Middle Aged
MH  - Young Adult
MH  - Thymic Stromal Lymphopoietin
OTO - NOTNLM
OT  - asthma
OT  - inhaled allergens
OT  - myeloid dendritic cells
OT  - plasmacytoid dendritic cells
EDAT- 2016/02/05 06:00
MHDA- 2017/07/06 06:00
CRDT- 2016/02/05 06:00
PHST- 2016/02/05 06:00 [entrez]
PHST- 2016/02/05 06:00 [pubmed]
PHST- 2017/07/06 06:00 [medline]
AID - 10.1164/rccm.201508-1623OC [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2016 Jul 15;194(2):169-77. doi: 
      10.1164/rccm.201508-1623OC.